Characteristics of TAV- and BAV-associated thoracic aortic aneurysms—Smooth muscle cell biology, expression profiling, and histological analyses

Blunder, Stefan; Meßner, Barbara; Aschacher, Thomas; Zeller, Iris; Türkcan, Adrian; Wiedemann, Dominik; Andreas, Martin; Blüschke, Gert; Laufer, Günther; Schachner, Thomas; Bernhard, David

doi:10.1016/j.atherosclerosis.2011.11.035

Cited by 63 publications

(62 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This finding is consistent with a recent paper, where the authors showed that TAA SMCs proliferate slowly compared to controls regardless of the age of the subject (Blunder et al, 2011).…”

Section: Sample Characterizationsupporting

confidence: 83%

A preliminary proteomic evaluation of smooth muscle cells in thoracic aortic aneurysms

Ayhan¹,

Baykal²,

Serhatlı³

et al. 2014

Turk J Biol

View full text Add to dashboard Cite

Aortic aneurysm is characterized as localized degeneration of the aorta leading to advanced weakening and widening of the vessel. While the exact mechanisms have yet to be determined, current studies indicate that the degradation of extracellular matrix (ECM) proteins and apoptosis of vascular smooth muscle cells (SMCs) may result in extendibility, dilation, and rupture of the vessel. Within the aortic wall, SMCs are implicated as key components involved in disease development, as numerous molecular changes have been reported to occur. Most current studies involve either investigation of proteins constituting a group or pathway in SMCs, or analyses of the whole aortic tissue. In order to determine which proteins are important in the development of thoracic aortic aneurysms (TAAs), we performed comparative proteomic analyses using cultured SMCs from TAAs versus controls. Label-free nano LC-MS/ MS analysis of cell extracts resulted in the identification of 256 proteins, 26 of which were differentially regulated by ≥1.4-fold. Both previously described and new proteins were identified that were involved in oxidative stress, ECM formation, energy metabolism, or the 14-3-3 pathway. Among these, differential expression of SerpinH1, a protease inhibitor for collagenases, was further verified via immunoblotting. Here we have attempted to shed light on the cellular mechanisms of TAAs.

show abstract

“…This finding is consistent with a recent paper, where the authors showed that TAA SMCs proliferate slowly compared to controls regardless of the age of the subject (Blunder et al, 2011).…”

Section: Sample Characterizationsupporting

confidence: 83%

A preliminary proteomic evaluation of smooth muscle cells in thoracic aortic aneurysms

Ayhan¹,

Baykal²,

Serhatlı³

et al. 2014

Turk J Biol

View full text Add to dashboard Cite

show abstract

“…Thereafter, one microgram of RNA was reverse transcribed using the IScript cDNA Synthesis Kit (BioRad, Hercules, CA, USA) and till further use stored at −20°C. All qRT-PCR were carried out on a BioRad iQ5 Cycler (BioRad Hercules, CA, USA) as described previously [22]. To control for variations in RNA quality and quantity, gene of interest (GOI) expression levels were normalized to the expression of β-actin.…”

Section: Methodsmentioning

confidence: 99%

Low Energy Shock Wave Therapy Induces Angiogenesis in Acute Hind-Limb Ischemia via VEGF Receptor 2 Phosphorylation

et al. 2014

Self Cite

View full text Add to dashboard Cite

ObjectivesLow energy shock waves have been shown to induce angiogenesis, improve left ventricular ejection fraction and decrease angina symptoms in patients suffering from chronic ischemic heart disease. Whether there is as well an effect in acute ischemia was not yet investigated.MethodsHind-limb ischemia was induced in 10–12 weeks old male C57/Bl6 wild-type mice by excision of the left femoral artery. Animals were randomly divided in a treatment group (SWT, 300 shock waves at 0.1 mJ/mm2, 5 Hz) and untreated controls (CTR), n = 10 per group. The treatment group received shock wave therapy immediately after surgery.ResultsHigher gene expression and protein levels of angiogenic factors VEGF-A and PlGF, as well as their receptors Flt-1 and KDR have been found. This resulted in significantly more vessels per high-power field in SWT compared to controls. Improvement of blood perfusion in treatment animals was confirmed by laser Doppler perfusion imaging. Receptor tyrosine kinase profiler revealed significant phosphorylation of VEGF receptor 2 as an underlying mechanism of action. The effect of VEGF signaling was abolished upon incubation with a VEGFR2 inhibitor indicating that the effect is indeed VEGFR 2 dependent.ConclusionsLow energy shock wave treatment induces angiogenesis in acute ischemia via VEGF receptor 2 stimulation and shows the same promising effects as known from chronic myocardial ischemia. It may therefore develop as an adjunct to the treatment armentarium of acute muscle ischemia in limbs and myocardium.

show abstract

“…Progressive ascending aortic dilation has been associated with activation of matrix metalloproteinases (MMPs) and down-regulation of their endogenous inhibitors (tissue inhibitor of matrix metalloproteinases [TIMPs]), which results in degradation of the aortic wall matrix and decreased elasticity (2,3,7). However, the mechanism responsible for initiating this process in BAV patients has not been elucidated.…”

mentioning

confidence: 98%

“…Both cell death and extracellular matrix (ECM) degradation have been identified as hallmarks of BAV aortopathy and aneurysm formation (2,7). Progressive ascending aortic dilation has been associated with activation of matrix metalloproteinases (MMPs) and down-regulation of their endogenous inhibitors (tissue inhibitor of matrix metalloproteinases [TIMPs]), which results in degradation of the aortic wall matrix and decreased elasticity (2,3,7).…”

mentioning

confidence: 99%

Progressive Aortic Dilation Is Regulated by miR-17–Associated miRNAs

Song

et al. 2016

Journal of the American College of Cardiology

View full text Add to dashboard Cite

show abstract

Characteristics of TAV- and BAV-associated thoracic aortic aneurysms—Smooth muscle cell biology, expression profiling, and histological analyses

Cited by 63 publications

References 31 publications

A preliminary proteomic evaluation of smooth muscle cells in thoracic aortic aneurysms

A preliminary proteomic evaluation of smooth muscle cells in thoracic aortic aneurysms

Low Energy Shock Wave Therapy Induces Angiogenesis in Acute Hind-Limb Ischemia via VEGF Receptor 2 Phosphorylation

Progressive Aortic Dilation Is Regulated by miR-17–Associated miRNAs

Contact Info

Product

Resources

About