Characteristics of the Infection of Tilletia laevis Kühn (syn. Tilletia foetida (Wallr.) Liro.) in Compatible Wheat

Abstract

Tilletia laevis Kühn (syn. Tilletia foetida (Wallr.) Liro.) causes wheat common bunt, which is one of the most devastating plant diseases in the world. Common bunt can result in a reduction of 80% or even a total loss of wheat production. In this study, the characteristics of T. laevis infection in compatible wheat plants were defined based on the combination of scanning electron microscopy, transmission electron microscopy and laser scanning confocal microscopy. We found T. laevis could lead to the abnormal growth of wheat tissues and cells, such as leakage of chloroplasts, deformities, disordered arrangements of mesophyll cells and also thickening of the cell wall of mesophyll cells in leaf tissue. What’s more, T. laevis teliospores were found in the roots, stems, flag leaves, and glumes of infected wheat plants instead of just in the ovaries, as previously reported. The abnormal characteristics caused by T. laevis may be used for early detection of this pathogen instead of molecular markers in addition to providing theoretical insights into T. laevis and wheat interactions for breeding of common bunt resistance.

ITRAQ-Based Proteomic Analysis of Wheat (Triticum aestivum) Spikes in Response to Tilletia controversa Kühn and Tilletia foetida Kühn Infection, Causal Organisms of Dwarf Bunt and Common Bunt of Wheat

“…PCR was done in triplicate with a total volume of 25 µL, including 1 µL of DNA (100 ng/µL), 1 µL of forward primer (10 µM), 1 µL of reverse primer (10 µM), 12.5 µL of master mix (TransGen, Beijing, China) and 9.5 µL of ddH 2 O (TransGen, Beijing, China). The PCR cycling conditions were followed as described by Ren et al, 2021 [ 31 ]. The primer sequences for T. controversa were ISSR859-140AF-5′TGGTGGTCGGGAAAGATTAGA-3′ and ISSR859-511AR- 5′-GGGACGAAGGCATCAAGAAG-3′ [ 32 ], and for T. foetida were L60F (5′-TCACTTCAAGGTCGTTCCCG-3′)/L60R (5-CGGGTCGAGGGGCGTAAACTTGA-3′) [ 33 ].…”