Characteristics of the rhizosphere bacterial community across different cultivation years in saline–alkaline paddy soils of Songnen Plain of China

Abstract

The characteristics of the rhizosphere microbial community across different cultivation years (from 1, 3, 5, 15, 20, and 50 years) in saline-alkaline paddy soils in Songnen Plain of China were investigated based on sequence variation of 16S rDNA using Illumina MiSeq sequencing. The results showed that the microbial community diversity varied across cultivation years, showing higher diversity in cultivation years >15 than in cultivation years <15. The dominant microbial community of the rhizosphere was mainly composed of Proteobacteria, Acidobacteria, Firmicutes, and Bacteroidetes. Furthermore, soil microbial diversity appeared to be affected directly by changes in soil properties corresponding to cultivated years. Diversity of Proteobacteria decreased as cultivated years increased; however, that of Acidobacteria showed the opposite direction. In addition, the soil microbial communities were clustered into two main groups: one from the sites cultivated for fewer than 15 years, and the other from the sites cultivated for more than 15 years. The abundance of nitrogen-fixing microorganisms in the soil sample was significantly higher in soils cultivated for under 15 years than in those cultivated for over 15 years (P < 0.05). Moreover, there was an obvious negative correlation between the cultivated years and Methanosarcina. Our findings on the dynamics of microbial community and its specific function in response to variable soil conditions are important for understanding and improving physical and chemical characteristics of saline-alkaline soil in Songnen Plain of China.

Improvement of subsoil physicochemical and microbial properties by short-term fallow practices

“…The proportion of Acidobacteria Gp6 increased in all treatments by the end of the year, and the highest abundance was observed in NS in October. A previous study showed that Acidobacteria Gp6 was negatively related to pH under saline-alkaline conditions (Cui et al, 2018), which was confirmed in our study. Therefore, the abundance of Acidobacteria Gp6 was relatively low under fallow conditions with manure (MS and MM).…”

Influence of Salt Stress on Growth of Spermosphere Bacterial Communities in Different Peanut (Arachis hypogaea L.) Cultivars

“…However, the potential role of seed-associated bacteria on seed germination under salt stress are poorly characterized. Some beneficial microbes in the rhizosphere bacterial community possess diverse metabolic capabilities and play crucial roles in plant adaptation to environmental stresses [24][25][26][27]. Thus, we hypothesize that spermosphere bacterial communities may also have implications for seed germination and survival under salt stress, which requires in-depth study.…”

Response of soil characteristics and bacterial communities to nitrogen fertilization gradients in a coastal salt‐affected agroecosystem

“…Under the salt-affected environment, soil microbial community composition was found to respond well to the interactive effect of amendment measures, including biochar-manure compost (Lu et al, 2015), biogas residue (Shi, Liang, Leng, Wang, & Wang, 2018), flue-gas desulfurization gypsum by-products (Li et al, 2012), saline water irrigation (Chen et al, 2017) and even duration of cultivation (Cui et al, 2018).…”