Characteristics of viable-but-nonculturable <i>Vibrio parahaemolyticus</i> induced by nutrient-deficiency at cold temperature

Yoon, Jae‐Hyun; Lee, Sun-Young

doi:10.1080/10408398.2019.1570076

Cited by 36 publications

(15 citation statements)

References 122 publications

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“…In addition, it has been suggested that the release of OMVs during transformation of H. pylori from a spiral to a coccoid form is associated with a reduction in cell size [24]. The similar process was also detected in VBNC Vibrio parahaemolyticus cells and was related to a starvation-dependent reduction in metabolic activity [90].…”

Section: Degradation Of Genetic Materials and Edna Amount In The Viabimentioning

confidence: 82%

Transformation of Helicobacter pylori into Coccoid Forms as a Challenge for Research Determining Activity of Antimicrobial Substances

Krzyżek

Grande

2020

Pathogens

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Morphological variability is one of the phenotypic features related to adaptation of microorganisms to stressful environmental conditions and increased tolerance to antimicrobial substances. Helicobacter pylori, a gastric mucosal pathogen, is characterized by a high heterogeneity and an ability to transform from a spiral to a coccoid form. The presence of the coccoid form is associated with the capacity to avoid immune system detection and to promote therapeutic failures. For this reason, it seems that the investigation for new, alternative methods combating H. pylori should include research of coccoid forms of this pathogen. The current review aimed at collecting information about the activity of antibacterial substances against H. pylori in the context of the morphological variability of this bacterium. The collected data was discussed in terms of the type of substances used, applied research techniques, and interpretation of results. The review was extended by a polemic on the limitations in determining the viability of coccoid H. pylori forms. Finally, recommendations which can help in future research aiming to find new compounds with a potential to eradicate H. pylori have been formulated.

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Section: Degradation Of Genetic Materials and Edna Amount In The Viabimentioning

confidence: 82%

Transformation of Helicobacter pylori into Coccoid Forms as a Challenge for Research Determining Activity of Antimicrobial Substances

Krzyżek

Grande

2020

Pathogens

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“…In seafood, V. parahaemolyticus is of particular interest because it is consistently involved in seafood-borne disease outbreaks [28]. Interestingly, V. parahaemolyticus can be induced into a viable-but-nonculturable (VBNC) state under low temperature and salinity conditions; bacteria, including Vp AHPND , are highly sensitive to freezing and refrigeration, both of which can lead to reductions in culture cells or non-detectable levels after several weeks [16,29].…”

Section: Discussionmentioning

confidence: 99%

Infectivity and Transmissibility of Acute Hepatopancreatic Necrosis Disease Associated Vibrio parahaemolyticus in Frozen Shrimp Archived at −80 °C

Lee

Jeon

Kim

et al. 2022

Fishes

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Acute hepatopancreatic necrosis disease (AHPND) caused by Vibrio parahaemolyticus (VpAHPND) has been reported in commodity shrimp, but the potential risk of its global spread via frozen shrimp in the shrimp trade is yet to be fully explored. We hypothesized that frozen shrimp with AHPND could be a source of VpAHPND transmission; thus, the infectivity of frozen shrimp with AHPND was evaluated using a shrimp bioassay. To prepare infected frozen shrimp, 12 Penaeus vannamei (average weight, 2 g) were exposed to VpAHPND by immersion in water with a VpAHPND concentration of 1.55 × 107 CFU mL−1; once dead, the shrimp were stored at −80 °C for further analysis. After two weeks, a PCR assay was used to confirm AHPND positivity in frozen shrimp (n = 2), and VpAHPND was reisolated from the hepatopancreases of these shrimp. For the infectivity test, 10 P. vannamei (average weight, 4 g) were fed with the hepatopancreases of VpAHPND -infected frozen shrimp (n = 10). After feeding, 70% of the shrimp died within 118 h, and the presence of VpAHPND was confirmed using a PCR assay and histopathology examination; moreover, VpAHPND was successfully reisolated from the hepatopancreases of the dead shrimp. We are the first to evaluate the potential transmissibility of VpAHPND in frozen shrimp, and our results suggest that frozen shrimp with AHPND are a potential source of disease spreading between countries during international trade.

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“…Небезынтересно отметить, что при более длительной инкубации (7-10 дней) клеток в условиях холодового стресса наблюдался обратный процесс -значительное снижение содержания основных ННЖК -С16:1, С18:1 (в 2-8 раз) и увеличение НЖК. Рядом авторов высказано предположение, что подобная модификация липидного состава в сочетании с изменением мембранного потенциала и уровня гидрофобности приводит к снижению текучести мембраны, индуцируя переход в некультивируемое состояние (крайнюю форму адаптации) при длительной инкубации в условиях низких температур Yoon, Lee, 2020].…”

Section: результаты и их обсуждениеunclassified

Influence of temperature stress on the spectrum of fatty acids of Vibrio cholerae strains

Shipko¹,

Дуванова²

2022

Вест. ПГУ. Биол.

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Under conditions of simulated temperature stress gas chromatography combined with mass spectrometry was used to study the spectrum of fatty acids of strains of Vibrio cholerae O1, O139, non O1/non O139 serogroups with a different set of pathogenicity determinants isolated from patients and from water samples of surface water bodies. Significant differences in the composition of fatty acids under cold and heat stress in the cells of the causative agent of cholera relative to control samples were revealed. The strains isolated from the water of surface reservoirs differed in the spectrum of fatty acids under cold stress from the strains isolated from patients. In strains isolated from water, a decrease in cultivation temperature to 23 °C was accompanied by the synthesis of docosanoic, tetracosanoic and hexacosanoic acids and to 4 °C - an increase in the amount of tetradecanoic and hexadecanoic acids. While in strains isolated from patients, a decrease in cultivation temperature caused a regular increase in hexadecenoic acids. and octadecenoic acids. The reaction to heat stress in most of the studied strains regardless of the set of pathogenicity determinants and the source of isolation had a general trend: an increase in the total amount of saturated fatty acids, the synthesis of trans-isomers of unsaturated fatty acids and the appearance of ω-alicyclic and iso-branched fatty acids. In addition to the remodeling of the fatty acid composition of the membrane under the influence of temperature stress, the synthesis of oxylipins, phenylpropanoids and terpenoids, possibly playing the role of adaptogens, was noted.

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Characteristics of viable-but-nonculturable Vibrio parahaemolyticus induced by nutrient-deficiency at cold temperature

Cited by 36 publications

References 122 publications

Transformation of Helicobacter pylori into Coccoid Forms as a Challenge for Research Determining Activity of Antimicrobial Substances

Transformation of Helicobacter pylori into Coccoid Forms as a Challenge for Research Determining Activity of Antimicrobial Substances

Infectivity and Transmissibility of Acute Hepatopancreatic Necrosis Disease Associated Vibrio parahaemolyticus in Frozen Shrimp Archived at −80 °C

Influence of temperature stress on the spectrum of fatty acids of Vibrio cholerae strains

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