Characteristics of virulence in human isolates of Yersinia enterocolitica

Schiemann, D. A.; Devenish, John; Toma, S

doi:10.1128/iai.32.1.400-403.1981

Cited by 51 publications

(36 citation statements)

References 9 publications

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“…The autoagglutination assay gives a relatively reliable presumptive indication of the pathogenic potential of Y. enterocolitica isolates. Complete agreement between the autoagglutination test results, presence of virulence plasmids, and mouse virulence has been reported by several authors (19,(27)(28)(29). However, a few false-negative and false-positive cultures have also been detected (8,18).…”

Section: Discussionsupporting

confidence: 67%

Relationship of virulence-associated autoagglutination to hemagglutinin production in Yersinia enterocolitica and Yersinia enterocolitica-like bacteria

Kapperud¹,

Lassen²

1983

Infect Immun

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A total of 339 Yersinia strains were tested for virulence-associated autoagglutination and hemagglutinin (HA) production. Autoagglutination was frequent among human clinical isolates belonging to Y. enterocolitica serogroups 0:3, 0:8, and 0:9 and among porcine isolates of serogroups 0:3 and 0:9. Positive autoagglutination results were also observed in serogroups 0:1 and 0:2, associated with outbreaks among chinchillas, hares, and goats. All of 164 environmental isolates were negative in the autoagglutination assay. When subcultured serially in nutrient broth, none of the clinical isolates produced HA at either 22 or 37°C, whereas 22.6% of the environmental yersiniae yielded mannose-resistant HA (MRHA). Six different MRHA patterns were recorded. Eighteen strains, all of which produced MRHA in nutrient broth, yielded similar MRHA patterns after cultivation on colonization factor agar. None of the autoagglutination-positive-(AA') strains produced MRHA in either nutrient broth or on colonization factor agar. In contrast, a distinct autoagglutination-associated MRHA was detected after growth in Eagle minimal essential medium. Comparison of AA' and autoagglutination-negative (AA-) derivatives of serogroups 0:2, 0:3, 0:5, 0:8, and 0:9 showed that all AA' cultures produced MRHA when grown in minimal essential medium at 37°C overnight, whereas no MRHA was observed with any of the AA-derivatives of the same serogroups. This autoagglutination associated MRHA reacted with guinea pig erythrocytes, but not with erythrocytes from seven other species.

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Section: Discussionsupporting

confidence: 67%

Relationship of virulence-associated autoagglutination to hemagglutinin production in Yersinia enterocolitica and Yersinia enterocolitica-like bacteria

Kapperud¹,

Lassen²

1983

Infect Immun

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“…Experimental procedures to determine Y. enterocolitica pathogenicity are costly, unreliable, or both. Although the virulence of certain serogroups can be demonstrated effectively in laboratory animals (4,16,18,21,23), such tests are generally not suitable for routine diagnostic use.…”

Section: Discussionmentioning

confidence: 99%

Differentiation between virulent and avirulent Yersinia enterocolitica isolates by using Congo red agar

Prpic¹,

Robins-Browne²,

Davey³

1983

J Clin Microbiol

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Cultivation of clinical isolates of Yersinia enterocolitica of diverse geographical origin on a medium containing 5 ,ug of Congo red per ml disclosed two colony types. These were designated CR+ and CR-according to their ability to bind Congo red. CR+ strains bore plasmids of between 40 and 50 megadaltons and were positive in several tests of Y. enterocolitica virulence, including autoagglutination, reduced growth on magnesium oxalate agar, resistance to the bactericidal effect of serum, and lethality for iron-overloaded mice. CRstrains were plasmidless and were negative in all these assays. The Congo red reaction provides a simple and efficient means of screening Y. enterocolitica for virulence and is the best available method for identifying individual plasmid-bearing colonies.

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“…Their survival was monitored for 21 days. The virulence of 0:3 isolates was established by examining for diarrhea following oral infection [12]. Mice, deprived of water overnight, were allowed to drink ad lib from a 50 ml water suspension of bacteria (1.…”

Section: Virulence Testsmentioning

confidence: 99%

“…Young rabbits, challenged orogastrically with isolates of Y. enterocolitica serotype 0:3 were shown to produce diarrhea with histological evidence of small bowel invasion by the pathogen [11]. Infection of mice with 0:3 isolates also has been shown to elicit diarrheal disease [12].…”

Section: Introductionmentioning

confidence: 99%

Association of colony morphology with virulence of Yersinia enterocolitica

Lazere

1983

FEMS Microbiology Letters

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Characteristics of virulence in human isolates of Yersinia enterocolitica

Abstract: Yersinia enterocolitica serotypes O:3, O:8, O:9, O:5,27, O:4,32, and O:21 were virulent as determined by autoagglutination and calcium dependency at 35 degrees C and ability to produce guinea pig conjunctivitis and mouse diarrhea.

Cited by 51 publications

References 9 publications

Relationship of virulence-associated autoagglutination to hemagglutinin production in Yersinia enterocolitica and Yersinia enterocolitica-like bacteria

Relationship of virulence-associated autoagglutination to hemagglutinin production in Yersinia enterocolitica and Yersinia enterocolitica-like bacteria

Differentiation between virulent and avirulent Yersinia enterocolitica isolates by using Congo red agar

Association of colony morphology with virulence of Yersinia enterocolitica

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