Characteristics of β-lactamases and their genes (blaA and blaB) in Yersinia intermedia and Y. frederiksenii

Mittal, Shilpi; Mallik, Sarita; Sharma, Sachin; Virdi, Jugsharan Singh

doi:10.1186/1471-2180-7-25

Cited by 10 publications

(7 citation statements)

References 44 publications

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“…They consisted of 20 genera, with the highest prevalence ascribed to clinically important constituents of this group such as Escherichia, Klebsiella, Yersinia , and Enterobacter . These genera are reservoirs of genes encoding antimicrobial resistance ( 9 , 10 , 13 ).…”

Section: Discussionmentioning

confidence: 99%

Microbiome and antimicrobial resistance genes in microbiota of cloacal samples from European herring gulls (Larus argentatus)

Merkevičienė

Ruzauskaite

Klimienė

et al. 2017

Journal of Veterinary Research

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IntroductionThe aim of the study was to determine microbiota in the cloacal samples of European herring gulls (Larus argentatus) and to compare a variety of genes encoding antimicrobial resistance in cultivable and non-cultivable bacteria.Material and MethodsCloacal samples from European herring gulls were collected from a Kaunas city dump. Cultivable microbiota were isolated, their microbial susceptibility was tested, and genes encoding antimicrobial resistance were detected. Additionally, a metagenomic study was performed using Next-Generation Sequencing (NGS).ResultsIn total, 697 different operational taxonomic units at genus level were detected; however, only 63 taxonomic units were detected at the amount of ≥0.1% of the total number of DNA copies. Catellicoccus marimammalium was found to have the highest prevalence. The bacterial amount of other genera was up to 5% with the most highly prevalent being Psychrobacter (4.7%), Helicobacter(4.5%), unclassified Enterococcaceae (3.2%), Pseudomonas (2.9%), and Brachyspira (2.6%).ConclusionsC. marimammalium are predominant microbiota in the cloacal samples of Larus argentatus. This species of gulls is a reservoir of bacteria carrying a wide-spectrum of genes encoding antimicrobial resistance. The same genes were detected in both cultivable microbiota and in the total DNA of the samples.

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Section: Discussionmentioning

confidence: 99%

Microbiome and antimicrobial resistance genes in microbiota of cloacal samples from European herring gulls (Larus argentatus)

Merkevičienė

Ruzauskaite

Klimienė

et al. 2017

Journal of Veterinary Research

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“…PCR methods have also been used to verify the pathogenicity of Y. enterocolitica isolates rapidly (Wren & Tabaqchali, 1990), in order to overcome the disadvantage of phenotypic classifications which are not always reliable (Kwaga & Iversen, 1992). The SDS-PAGE of whole-cell proteins was used in several studies (for example, Chart & Cheasty, 2006;Mittal, Mallik, Sharma, & Virdi, 2007).…”

Section: Introductionmentioning

confidence: 99%

Occurrence and characterization of Aeromonas hydrophila and Yersinia enterocolitica in minimally processed fresh vegetable salads

2010

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“…For example, organisms not yet shown to produce a functional AmpCtype enzyme but with identified ampC genes include such diverse bacteria as Agrobacterium tumefaciens (110), Coxiella burnetii (GenBank accession number YP_001424134), Legionella pneumophila (56), Rickettsia felis (239), and Sinorhizobium meliloti (127). For other organisms, supportive MIC or enzymatic but not structural data are available for the presence of AmpC ␤-lactamase, including Enterobacter sakazakii (258), Ewingella americana (311), Providencia rettgeri (207), and several species of Serratia (306,307) and Yersinia (215,288,313). The phylum Proteobacteria contains the largest number, but at least one acid-fast actinobacte-rium also produces AmpC ␤-lactamase.…”

Section: Distributionmentioning

confidence: 99%

AmpC β-Lactamases

2009

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SUMMARY AmpC β-lactamases are clinically important cephalosporinases encoded on the chromosomes of many of the Enterobacteriaceae and a few other organisms, where they mediate resistance to cephalothin, cefazolin, cefoxitin, most penicillins, and β-lactamase inhibitor-β-lactam combinations. In many bacteria, AmpC enzymes are inducible and can be expressed at high levels by mutation. Overexpression confers resistance to broad-spectrum cephalosporins including cefotaxime, ceftazidime, and ceftriaxone and is a problem especially in infections due to Enterobacter aerogenes and Enterobacter cloacae, where an isolate initially susceptible to these agents may become resistant upon therapy. Transmissible plasmids have acquired genes for AmpC enzymes, which consequently can now appear in bacteria lacking or poorly expressing a chromosomal blaAmpC gene, such as Escherichia coli, Klebsiella pneumoniae, and Proteus mirabilis. Resistance due to plasmid-mediated AmpC enzymes is less common than extended-spectrum β-lactamase production in most parts of the world but may be both harder to detect and broader in spectrum. AmpC enzymes encoded by both chromosomal and plasmid genes are also evolving to hydrolyze broad-spectrum cephalosporins more efficiently. Techniques to identify AmpC β-lactamase-producing isolates are available but are still evolving and are not yet optimized for the clinical laboratory, which probably now underestimates this resistance mechanism. Carbapenems can usually be used to treat infections due to AmpC-producing bacteria, but carbapenem resistance can arise in some organisms by mutations that reduce influx (outer membrane porin loss) or enhance efflux (efflux pump activation).

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Characteristics of β-lactamases and their genes (blaA and blaB) in Yersinia intermedia and Y. frederiksenii

Cited by 10 publications

References 44 publications

Microbiome and antimicrobial resistance genes in microbiota of cloacal samples from European herring gulls (Larus argentatus)

Microbiome and antimicrobial resistance genes in microbiota of cloacal samples from European herring gulls (Larus argentatus)

Occurrence and characterization of Aeromonas hydrophila and Yersinia enterocolitica in minimally processed fresh vegetable salads

AmpC β-Lactamases

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