Characteristics of γ-hexachlorocyclohexane biodegradation by a nitrogen-fixing cyanobacterium, Anabaena azotica

Zhang, Hangjun; Hu, Ciming; Jia, Xiao‐Bin; Xu, Yi; Wu, Chenjie; Chen, Lina; Wang, Fengping

doi:10.1007/s10811-011-9670-7

Cited by 39 publications

(8 citation statements)

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“…The Anabaena flos-aquae strain 4054 can decompose endocrine-disrupting pollutants, such as phthalate esters [17] . Anabaena azotica , another common cyanobacterium, can effectively degrade the organochlorine pesticide γ-hexachlorocyclohexane (lindane) [18] . Therefore, cyanobacterial species with degradation functions may be a potential choice for PCB degradation.…”

Section: Introductionmentioning

confidence: 99%

Proteomic Strategy for the Analysis of the Polychlorobiphenyl-Degrading Cyanobacterium Anabaena PD-1 Exposed to Aroclor 1254

et al. 2014

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The cyanobacterium Anabaena PD-1, which was originally isolated from polychlorobiphenyl (PCB)-contaminated paddy soils, has capabilities for dechlorinatin and for degrading the commercial PCB mixture Aroclor 1254. In this study, 25 upregulated proteins were identified using 2D electrophoresis (2-DE) coupled with matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS). These proteins were involved in (i) PCB degradation (i.e., 3-chlorobenzoate-3,4-dioxygenase); (ii) transport processes [e.g., ATP-binding cassette (ABC) transporter substrate-binding protein, amino acid ABC transporter substrate-binding protein, peptide ABC transporter substrate-binding protein, putrescine-binding protein, periplasmic solute-binding protein, branched-chain amino acid uptake periplasmic solute-binding protein, periplasmic phosphate-binding protein, phosphonate ABC transporter substrate-binding protein, and xylose ABC transporter substrate-binding protein]; (iii) energetic metabolism (e.g., methanol/ethanol family pyrroloquinoline quinone (PQQ)-dependent dehydrogenase, malate-CoA ligase subunit beta, enolase, ATP synthase β subunit, FOF1 ATP synthase subunit beta, ATP synthase α subunit, and IMP cyclohydrolase); (iv) electron transport (cytochrome b6f complex Fe-S protein); (v) general stress response (e.g., molecular chaperone DnaK, elongation factor G, and translation elongation factor thermostable); (vi) carbon metabolism (methanol dehydrogenase and malate-CoA ligase subunit beta); and (vii) nitrogen reductase (nitrous oxide reductase). The results of real-time polymerase chain reaction showed that the genes encoding for dioxygenase, ABC transporters, transmembrane proteins, electron transporter, and energetic metabolism proteins were significantly upregulated during PCB degradation. These genes upregulated by 1.26- to 8.98-fold. These findings reveal the resistance and adaptation of cyanobacterium to the presence of PCBs, shedding light on the complexity of PCB catabolism by Anabaena PD-1.

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Section: Introductionmentioning

confidence: 99%

Proteomic Strategy for the Analysis of the Polychlorobiphenyl-Degrading Cyanobacterium Anabaena PD-1 Exposed to Aroclor 1254

et al. 2014

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“…The incubation temperature of 30 °C has been found as optimum temperature for lindane degradation (Zhang et al 2010, 2012; Salam et al 2013). However, rapid degradation of lindane by Sphingobium strains has been reported by Zheng et al (2011) even at low temperature (4 °C) indicating that lindane degradation could be achieved even in colder contaminated regions.…”

Section: Resultsmentioning

confidence: 99%

Degradation study of lindane by novel strains Kocuria sp. DAB-1Y and Staphylococcus sp. DAB-1W

Kumar

Sharma

2016

Bioresour. Bioprocess.

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BackgroundThis study was carried out to isolate and characterize the bacterial strains from lindane-contaminated soil and they were also assessed for their lindane-degrading potential.MethodsIn this study the enrichment culture method was used for isolation of lindane degrading bacterial isolates, in which the mineral salt medium (MSM) supplemented with different concentrations of lindane was used. Further, the screening for the potential lindane degrading isolates was done using the spray plate method and colorimetric dechlorinase enzyme assay. The selected isolates were also studied for their growth response under varying range of temperature, pH, and NaCl. The finally selected isolates DAB-1Y and DAB-1W showing best lindane degradation activity was further subjected to biochemical characterization, microscopy, degradation/kinetic study, and 16S rDNA sequencing. The strain identification were performed using the biochemical characterization, microscopy and the species identifies by 16S rDNA sequence of the two isolates using the standard 16S primers, the 16 S rRNA partial sequence was analyzed through BLAST analysis and phylogenetic tree was generated based on UGPMA clustering method using MEGA7 software. This shows the phylogenetic relationship with the related strains. The two isolates of this study were finally characterized as Kocuria sp. DAB-1Y and Staphylococcus sp. DAB-1W, and their 16S rRNA sequence was submitted to GenBank database with accession numbers, KJ811539 and KX986577, respectively.ResultsOut of the 20 isolates, the isolates DAB-1Y and DAB-1W exhibited best lindane-degrading activity of 94 and 98%, respectively, recorded after 8 days of incubation. The optimum growth was observed at temperature 30 °C, pH 7, and 5% NaCl observed for both isolates. Of the four isomers of hexachlorocyclohexane, isomer α and γ were the fastest degrading isomers, which were degraded up to 86 and 94% by isolates DAB-1Y and up to 93 and 98% by DAB-1W, respectively, reported after 8 days incubation. Isomer β was highly recalcitrant in which maximum 35 and 32% lindane degradation was observed even after 28 days incubation by isolates, DAB-1Y and DAB-1W, respectively. At lower lindane concentrations (1–10 mg/L), specific growth rate increased with increase in lindane concentration, maximum being 0.008 and 0.006/day for DAB-1Y and DAB-1W, respectively. The 16 S rRNA partial sequence of isolate DAB-1Y showed similarity with Kocuria sp. by BLAST analysis and was named as Kocuria sp. DAB-1Y and DAB-IW with Staphylococcus sp. DAB-1W. The 16S rDNA sequence of isolate DAB-1Y and DAB-1W was submitted to online at National Centre of Biotechnology Information (NCBI) with GenBank accession numbers, KJ811539 and KX986577, respectively.ConclusionsThis study has demonstrated that Kocuria sp. DAB-1Y and Staphylococcus sp. DAB-1W were found efficient in bioremediation of gamma-HCH and can be utilized further for biodegradation of environmental contamination of lindane and can be utilized in bioremediation program.Electronic supplement...

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“…Cyanobacterial species, such as Anabaena flosaquae strain 4054, can degrade the endocrine-disrupting pollutants, like phthalate esters [110]. Another common cyanobacterium, Anabaena azotica, can effectively decompose the organochlorine pesticide γ-hexachlorocyclohexane (lindane) [111].…”

Section: Environmental Implicationsmentioning

confidence: 99%

Cyanobacteria: Review of Current Potentials and Applications

Choo²,

et al. 2020

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Continual increases in the human population and growing concerns related to the energy crisis, food security, disease outbreaks, global warming, and other environmental issues require a sustainable solution from nature. One of the promising resources is cyanobacteria, also known as blue-green algae. They require simple ingredients to grow and possess a relatively simple genome. Cyanobacteria are known to produce a wide variety of bioactive compounds. In addition, cyanobacteria’s remarkable growth rate enables its potential use in a wide range of applications in the fields of bioenergy, biotechnology, natural products, medicine, agriculture, and the environment. In this review, we have summarized the potential applications of cyanobacteria in different areas of science and development, especially related to their use in producing biofuels and other valuable co-products. We have also discussed the challenges that hinder such development at an industrial level and ways to overcome such obstacles.

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Characteristics of γ-hexachlorocyclohexane biodegradation by a nitrogen-fixing cyanobacterium, Anabaena azotica

Cited by 39 publications

References 23 publications

Proteomic Strategy for the Analysis of the Polychlorobiphenyl-Degrading Cyanobacterium Anabaena PD-1 Exposed to Aroclor 1254

Proteomic Strategy for the Analysis of the Polychlorobiphenyl-Degrading Cyanobacterium Anabaena PD-1 Exposed to Aroclor 1254

Degradation study of lindane by novel strains Kocuria sp. DAB-1Y and Staphylococcus sp. DAB-1W

Cyanobacteria: Review of Current Potentials and Applications

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