Characteristics, Yield, Flow Cytometer, DNA Fragmentation, and Comet Assay Parameters of Goat Spermatozoa in Semen of Zaraibi and Baladi Bucks at Young and Old Ages

Zaghloul, H. K.; El-Maghraby, M. M.; Badawy, Ahmed; Yousif, Ahmed I.

doi:10.21608/jappmu.2021.95942.1021

Cited by 1 publication

(1 citation statement)

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“…This is confirmed by a study conducted with three different breeds, which demonstrated that Alpine bucks had higher quantitative sperm parameters (sperm volume, sperm concentration) but lower qualitative characteristics (sperm motility) compared to the same parameters for Saanen and Damascus breeds [6]. Similar to the results of our study, there was found no difference in sperm DNA fragmentation rate between the Zaraibi and Baladi goat breeds [19].…”

Section: Resultssupporting

confidence: 91%

Reproductive characteristics of Saanen and Alpine bucks

Bogdaniuk,

Garkavii,

Petrushko

2023

Bìol. Tvarin

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Genetic variations among breeds within a species can impact not only productivity traits, such as milk yield and quality, but also animal health, including fertility. This study aimed to compare the reproductive characteristics of bucks from the Saanen and Alpine breeds. Sperm concentration and motility were assessed using light microscopy, viability was determined using eosin-nigrosin staining, and morphological parameters were evaluated using the Spermac Stain method. DNA fragmentation was measured using the Halosperm kit. Artificial insemination of goats was conducted with fresh semen during natural estrus. Statistical analysis was performed using the Graph Pad Prism software. The results revealed that Alpine bucks exhibited significantly higher semen volume, sperm concentration, viability, and motility (P<0.05). No significant differences (P≥0,05) were observed between the breeds regarding the number of spermatozoa with normal morphology and the rate of DNA fragmentation. Cryobiological analysis of spermatozoa from Saanen bucks suggested a higher cryoresistance compared to the Alpine breed. Following artificial insemination of goats, the pregnancy rate for the Saanen breed was 61.8%, which was twice as high as that observed in Alpine goats — 28.8% (P<0.05). These findings demonstrate significant differences in reproductive characteristics between Saanen and Alpine goats. Despite superior sperm characteristics, the pregnancy rate after artificial insemination was significantly lower in the Alpine breed compared to the Saanen breed. Consequently, it is crucial to consider these variations in essential reproductive characteristics when implementing breeding programs and employing reproductive biotechnology in animal husbandry to ensure their successful application and effectiveness.

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Section: Resultssupporting

confidence: 91%