Characterization and Absorption Kinetics of a Novel Multifunctional Nanoliposome Stabilized by Sea Cucumber Saponins Instead of Cholesterol

Abstract

Cholesterol was usually used to stabilize liposome, although there have been controversies on the relationship between dietary cholesterol and health. The present study aimed to prepare a novel multifunctional nanoliposomes stabilized by sea cucumber-derived saponins using ultrasound-assisted film dispersion method. A novel uniform liposome with a mass ratio of egg yolk lecithin/sea cucumber saponins at 75:25 was successfully prepared to encapsulate saponin, and the particle size was 164.8 ± 1.70 nm with a PDI value of 0.214 ± 0.022 and zeta potential of −15.97 ± 1.23 mV. The digestion and absorption results in vivo showed that the dietary saponins in liposome form could delay the peak time of saponins and prolong their residence time in the serum. Moreover, saponins were more easily converted into their corresponding metabolites after administration with saponins in the liposome form. The novel liposome as an efficient carrier with multiple functions had great potential in the development of functional food and biomedical applications.

Build-Up of a 3D Organogel Network within the Bilayer Shell of Nanoliposomes. A Novel Delivery System for Vitamin D3: Preparation, Characterization, and Physicochemical Stability

“…Zeta potential is a significant indicator to predict the physical stability of colloidal suspensions. 10 In this regard, the range of −30 to +30 mV shows the instability of a colloid, and the degree of instability rises when the zeta potential approaches zero. 8 The potential values of fresh control samples and those with the gelled shell structure were −52 and −65 mV, respectively ( Figure 5 ).…”

“… 5 − 7 The physical stability of liposomes strongly depends on molecular ordering, packing, and dynamics of acyl chains in the bilayer, charge intensity, as well as the physical state–gel or fluid–and composition of lipids. 8 − 10 Therefore, many efforts have been made to overcome these challenging tasks, including liposome coating with hydrogel networks, 11 surface modification of vesicles with polymeric matrices using an electrostatic layer-by-layer technique, 12 , 13 and compositional change of bilayer membranes by sterols, 14 polyethylene glycol, 15 and emulsifiers. 16 Moreover, the same type and concentration of lipid materials with different liposome preparation methods lead to different properties, such as storage stability, encapsulation efficiency, and bilayer permeability.…”

Preparation and Characterization of Astaxanthin-loaded Liposomes Stabilized by Sea Cucumber Sulfated Sterols Instead of Cholesterol

“…Digestion and absorption study of free astaxanthin and encapsulated astaxanthin were performed by animal experimental procedures previously described 1) according to the guidelines of the Ethical Committee of Experimental Animal Care at the Ocean University of China. Balb/c male mice were offered with commercial feed for mice chow diet and tap water and were maintained at 65±15％ relative humidity at 25℃ with a 12 h light-dark cycle facility.…”

“…In recent decades, liposomes have been commonly used as a carrier system for encapsulation of bioactive ingredients 1,2) . Phospholipids and cholesterol are generally the main components of traditional liposomes.…”

Advances in the Study of Marine Products with Lipid-Lowering Properties

“…Saponins are interesting bioactive compounds with marine animal origin. The sea cucumber saponins, for example, are triterpenoid glycosides composed of triterpenoids and carbohydrate parts [105]. The effects of saponins on the hyperlipidemia have been extensively studied [106].…”