Characterization and Action Mechanism Analysis of VvmiR156b/c/d-VvSPL9 Module Responding to Multiple-Hormone Signals in the Modulation of Grape Berry Color Formation

Su, Zhiyuan; Wang, Xicheng; Xuan, Xuxian; Sheng, Zilu; Jia, Haoran; Naseri, Emal; Liu, Zhongjie; Zheng, Ting; Wang, Chen; Fang, Jinggui

doi:10.3390/foods10040896

Cited by 20 publications

(13 citation statements)

References 55 publications

(47 reference statements)

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“…In fact, phytohormones play an important role in regulating the ripening process and secondary metabolism of grape berries. Previous studies have shown that the application of ABA, BRs and JA on grape berries before the color transition period can promote the accumulation of phenylpropanoid metabolites [17][18][19][20][21][22]. The spraying of IAA and Forchlorfenuron (CPPU) can delay the maturation process and change the flow of phenylpropane metabolic pathways [23,24].…”

Section: Introductionmentioning

confidence: 99%

A One-Step Polyphenol Removal Approach for Detection of Multiple Phytohormones from Grape Berry

et al. 2022

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Phytohormones play an important role in regulating the maturation process and the quality-related metabolite accumulation of fruits, and their concentration variation has always been concerned during fruit development. However, berry fruits, such as grape berries, are rich in a large number of secondary metabolites, which brings great challenges to the isolation and determination of hormones. In this work, we used grapes as experimental materials and proposed a solid-phase extraction (SPE) protocol to efficiently isolate multiple hormones from phenol-rich matrix using a mixture of dichloromethane, methanol and formic acid as eluent. A highly sensitive method based on ultra-high-performance liquid chromatography–tandem mass spectrometry (UHPLC-MS/MS) was developed to quantify a total of 11 plant growth regulators, including the recognized phytohormones, in grape pericarp and seed. The established method showed satisfactory precision (RSD < 11.3%) and linearity (R2 > 0.9980). The limits of detection (LOD) and the limit of quantification (LOQ) were 0.001–0.75 ng/mL and 0.004–2.5 ng/mL, respectively. The recovery for the three levels of analytes spiked ranged from 63% to 118%, and the matrix effect was between 73% and 119%. Finally, the proposed method was applied to investigate the dynamic hormone concentration in Vitis vinifera L. cv. Cabernet Sauvignon berries from different vineyards, and assess the changes in endogenous hormones in grapes after treatment with exogenous growth regulators. We found that the contents of IP, ABA and IAA in pericarp and IP, IAA, IBA and SA in seed were significantly down-regulated after 10 days of treatment with NAA concentrations of 10 mg/L and 40 mg/L. In conclusion, this method helps to elucidate the role played by phytohormones in the maturation process and the accumulation of quality-related metabolites in phenol-rich fruits.

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Section: Introductionmentioning

confidence: 99%

A One-Step Polyphenol Removal Approach for Detection of Multiple Phytohormones from Grape Berry

et al. 2022

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“…In addition to MYB and WD40 transcription factors and structural genes, SPL is also involved in the synthesis of anthocyanin. VvmiR156b/c/d‐mediated VvSPL9 participated in the formation of grape ( V. vinifera ) color in response to multi‐hormone signals (Su et al, 2021 ). The expression of VcSPL12 significantly enhanced the accumulation of chlorophyll and altered the level of expression of several genes associated with chlorophyll that are involved with the coloration of blueberry ( Vaccinium spp.)…”

Section: Discussionmentioning

confidence: 99%

“…color in response to multi-hormone signals (Su et al, 2021). The expression of VcSPL12 significantly enhanced the accumulation of chlorophyll and altered the level of expression of several genes associated with chlorophyll that are involved with the coloration of blueberry (Vaccinium spp.)…”

Section: Anthocyanin Content and Related Gene Expression In Purple Po...mentioning

confidence: 99%

Identification of microRNAs and their target genes related to the accumulation of anthocyanin in purple potato tubers (Solanum tuberosum)

et al. 2022

Plant Direct

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MicroRNAs (miRNAs) are types of endogenous non‐coding small RNAs found in eukaryotes that are 18–25 nucleotides long. miRNAs are considered to be key regulatory factors of the expression of target mRNA. The roles of miRNAs involved in the regulation of anthocyanin accumulation in pigmented potatoes have not been systematically reported. In this study, the differentially expressed miRNAs and their target genes involved in the accumulation of anthocyanin during different developmental stages in purple potato ( Solanum tuberosum L.) were identified using small RNA (sRNA) and degradome sequencing. A total of 275 differentially expressed miRNAs were identified in the sRNA libraries. A total of 69,387,200 raw reads were obtained from three degradome libraries. The anthocyanin responsive miRNA–mRNA modules were analyzed, and 37 miRNAs and 23 target genes were obtained. Different miRNAs regulate the key enzymes of anthocyanin synthesis in purple potato. The structural genes included phenylalanine ammonia lyase, chalcone isomerase, flavanone 3‐hydroxylase, and anthocyanidin 3‐ O ‐glucosyltransferase. The regulatory genes included WD40, MYB, and SPL9. stu‐miR172e‐5p_L‐1R‐1, stu‐miR828a, stu‐miR29b‐4‐5p, stu‐miR8019‐5p_L‐4R‐3, stu‐miR396b‐5p, stu‐miR5303f_L‐7R + 2, stu‐miR7997a_L‐3, stu‐miR7997b_L‐3, stu‐miR7997c_L + 3R‐5_2ss2TA3AG, stu‐miR156f‐5p_L + 1, stu‐miR156a, stu‐miR156a_R‐1, stu‐miR156e, stu‐miR858, stu‐miR5021, stu‐miR828 and their target genes were validated by qRT‐PCR. They play important roles in the coloration and accumulation of purple potatoes. These results provide new insights into the biosynthesis of anthocyanins in pigmented potatoes.

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“…The injected tobacco was kept in the dark for 2 d and the leaves were treated with water and 50 μM GA. On day three, leaves were collected. Each treatment was repeated with five leaves and the experiment was repeated three times (Su et al., 2021).…”

Section: Methodsmentioning

confidence: 99%

Characterization and regulatory mechanism analysis of VvmiR156a–VvAGL80 pair during grapevine flowering and parthenocarpy process induced by gibberellin

Xuan

Sheng

et al. 2021

The Plant Genome

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MicroRNA156 (miR156) is an important conserved miRNA family in plants. Recently, we revealed VvmiR156a could involve in the modulation of gibberellin (GA)‐mediated flower and berry development process of grapevine (Vitis vinifera L.). However, how to manipulate this process is unclear. For this, we used the GA‐induced grapevine parthenocarpy system to investigate the regulatory roles of VvmiR156a during this process. Here, we cloned the mature and precursor sequences of VvmiR156a in Wink grape and identified its potential target gene VvAGL80, which belongs to the MADS‐box gene family. Moreover, using RNA ligase‐mediated 5′ rapid amplification of cDNA ends (RLM‐RACE) and poly(A)polymerase‐mediated 3′ rapid amplification of cDNA (PPM‐RACE) technologies, it confirmed that VvAGL80 was the true target gene of VvmiR159a. Analysis of promoter cis‐elements and β‐glucuronidase (GUS) staining showed that both VvmiR156a and VvAGL80 contained GA‐responsive elements and could respond to GA treatments. Quantitative real‐time–polymerase chain reaction (qRT‐PCR) analysis exhibited the VvmiR156a and VvAGL80 showed opposite expression trends during grapevine flower and berry development, indicating that VvmiR156a negatively regulated the expression of VvAGL80 during this process. After GA treatment, the expression of miR156 in flowers was downregulated significantly, while that of VvAGL80 was upregulated, thereby accelerating grapevine flowering. Furthermore, GA treatment enhanced the negative regulation of VvmiR156a on VvAGL80 in seed, especially at the seed‐coat hardening stage, which was the key period of seed growth and development. Our findings enriched the knowledge of the regulatory mechanism of the miRNA‐mediated grapevine parthenocarpy process.

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Characterization and Action Mechanism Analysis of VvmiR156b/c/d-VvSPL9 Module Responding to Multiple-Hormone Signals in the Modulation of Grape Berry Color Formation

Cited by 20 publications

References 55 publications

A One-Step Polyphenol Removal Approach for Detection of Multiple Phytohormones from Grape Berry

A One-Step Polyphenol Removal Approach for Detection of Multiple Phytohormones from Grape Berry

Identification of microRNAs and their target genes related to the accumulation of anthocyanin in purple potato tubers (Solanum tuberosum)

Characterization and regulatory mechanism analysis of VvmiR156a–VvAGL80 pair during grapevine flowering and parthenocarpy process induced by gibberellin

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