Characterization and adsorption of a Lactobacillus plantarum virulent phage

Chen, X.; Guo, Jing; Liu, Y.; Chai, Shiyu; Ma, Ruixin; Munguntsetseg, B.

doi:10.3168/jds.2018-16019

Cited by 20 publications

(19 citation statements)

References 26 publications

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“…Phage thermostability was examined by incubating high titer phage stocks (≥10 8 PFU per mL) at 4, 37, 50, 55, 60, and 70 °C for 60 min and pH tolerance was examined by incubating over a pH range of 1.0 to 12.0 for 60 min at 37 °C, as previously described, with a few modifications [ 38 , 39 ]. Phage titers were determined by the double-layer agar plate as per above.…”

Section: Methodsmentioning

confidence: 99%

Effects of the Newly Isolated T4-like Phage on Transmission of Plasmid-Borne Antibiotic Resistance Genes via Generalized Transduction

Zhang

Shen

et al. 2021

Viruses

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Bacteriophages are the most abundant biological entities on earth and may play an important role in the transmission of antibiotic resistance genes (ARG) from host bacteria. Although the specialized transduction mediated by the temperate phage targeting a specific insertion site is widely explored, the carrying characteristics of “transducing particles” for different ARG subtypes in the process of generalized transduction remains largely unclear. Here, we isolated a new T4-like lytic phage targeting transconjugant Escherichia coli C600 that contained plasmid pHNAH67 (KX246266) and encoded 11 different ARG subtypes. We found that phage AH67C600_Q9 can misload plasmid-borne ARGs and package host DNA randomly. Moreover, for any specific ARG subtype, the carrying frequency was negatively correlated with the multiplicity of infection (MOI). Further, whole genome sequencing (WGS) identified that only 0.338% (4/1183) of the contigs of an entire purified phage population contained ARG sequences; these were floR, sul2, aph(4)-Ia, and fosA. The low coverage indicated that long-read sequencing methods are needed to explore the mechanism of ARG transmission during generalized transduction.

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Section: Methodsmentioning

confidence: 99%

Effects of the Newly Isolated T4-like Phage on Transmission of Plasmid-Borne Antibiotic Resistance Genes via Generalized Transduction

Zhang

Shen

et al. 2021

Viruses

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show abstract

“…Phage thermostability was examined by incubating phage filtrates at 4, 37, 50, 55, 60, and 70 °C for 60 min. The pH stability was examined by incubating filtrates over a pH range of 1 to 12 for 24 h at 37 °C as previously described with a few modifications [ 25 ]. Phage titers were assayed by plating in soft agar (see above) and each test was performed in triplicate.…”

Section: Methodsmentioning

confidence: 99%

Evaluation of Potential ARG Packaging by Two Environmental T7-Like Phage during Phage-Host Interaction

Liu

Feng

et al. 2020

Viruses

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The increase in antimicrobial resistance is a threat to both human and animal health. The transfer of antibiotic resistance genes (ARG) via plasmids has been studied in detail whereas the contribution of bacteriophage-mediated ARG transmission is relatively little explored. We isolated and characterized two T7-like lytic bacteriophages that infected multidrug-resistant Escherichia coli hosts. The morphology and genomic analysis indicated that both phage HZP2 and HZ2R8 were evolutionarily related and their genomes did not encode ARGs. However, ARG-like raw reads were detected in offspring sequencing data with a different abundance level implying that potential ARG packaging had occurred. PCR results demonstrated that six fragments of genes (qnrS, cmlA, tetM, blaTEM, sul3, mcr-1) were potentially packaged by phage HZP2 and four (qnrS, cmlA, blaTEM, mcr-1) by phage HZ2R8. Further quantitative results showed that ARG abundance hierarchies were similar. The gene blaTEM was the most abundant (up to 1.38 × 107 copies/mL) whereas cmlA and qnrS were the least. Moreover, the clinically important mcr-1 gene was the second most abundant ARG indicating a possibility for spread through generalized transduction. Together, our results indicated that these structurally similar phage possessed similar characteristics and potential packaging during phage-host interaction displayed an ARG preference rather than occurring randomly.

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“…Temperature stability assay. The effect of temperature on phage viability was assessed according to the method described by Chen et al (23). The phage suspension (1.1 × 10 10 PFU/ml) was inoculated into a microtube containing BHI broth (at a final volume of 1mL) and incubated at 30, 37, 45, 50, and 55°C for 30 min at pH 7.…”

Section: Genementioning

confidence: 99%

Biological characteristics and anti-biofilm activity of a lytic phage against vancomycin-resistant Enterococcus faecium

Goodarzi

Hallajzadeh

Sholeh

et al. 2021

IJM

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Background and Objectives: An important leading cause of the emergence of vancomycin-resistant enterococci, especially Enterococcus faecium, is the inefficiency of antibiotics in the elimination of drug-resistant pathogens. Consequently, the need for alternative treatments is more necessary than ever. Materials and Methods: A highly effective bacteriophage against vancomycin-resistant E. faecium called vB-EfmS-S2 was isolated from hospital sewage. The biological properties of phage S2 and its effect on biofilm structures were determined. Results: Phage S2 was specifically capable of lysing a wide range of clinical E. faecium isolates. According to Electron mi- croscopy observations, the phage S2 belonged to the Siphoviridea family. Suitable pH spectra for phage survival was 5-11, at which the phage showed 100% activity. The optimal temperature for phage growth was 30-45°C, with the highest growth at 37°C. Based on one-step growth curve results, the latent period of phage S2 was 14 min with a burst size of 200 PFU/ml. The phage S2 was also able to tolerate bile at concentrations of 1 and 2% and required Mg2+ for an effective infection cycle. Biofilms were significantly inhibited and disrupted in the presence of the phage. Conclusion: According to the results, phage S2 could potentially be an alternative for the elimination and control of vancomycin-resistant E. faecium biofilm.

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Characterization and adsorption of a Lactobacillus plantarum virulent phage

Cited by 20 publications

References 26 publications

Effects of the Newly Isolated T4-like Phage on Transmission of Plasmid-Borne Antibiotic Resistance Genes via Generalized Transduction

Effects of the Newly Isolated T4-like Phage on Transmission of Plasmid-Borne Antibiotic Resistance Genes via Generalized Transduction

Evaluation of Potential ARG Packaging by Two Environmental T7-Like Phage during Phage-Host Interaction

Biological characteristics and anti-biofilm activity of a lytic phage against vancomycin-resistant Enterococcus faecium

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