2020
DOI: 10.1038/s41598-020-71178-5
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Characterization and analysis of full-length transcriptomes from two grasshoppers, Gomphocerus licenti and Mongolotettix japonicus

Abstract: Acrididae are diverse in size, body shape, behavior, ecology and life history; widely distributed; easy to collect; and important to agriculture. they represent promising model candidates for functional genomics, but their extremely large genomes have hindered this research; establishing a reference transcriptome for a species is the primary means of obtaining genetic information. Here, two Acrididae species, Gomphocerus licenti and Mongolotettix japonicus, were selected for full-length (fL) pacBio transcripto… Show more

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Cited by 5 publications
(4 citation statements)
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References 69 publications
(93 reference statements)
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“…To address this problem, sequencing depth was increased in the current study to produce a sufficient amount of effective sequences. The number of protein-expressing genes in the functional gene dataset observed in the current study was the same as that found in previous studies ( Yuan et al, 2020 ). The proportion of annotated functional genes was increased, and the average length of genes was markedly improved.…”
Section: Discussionsupporting
confidence: 88%
See 1 more Smart Citation
“…To address this problem, sequencing depth was increased in the current study to produce a sufficient amount of effective sequences. The number of protein-expressing genes in the functional gene dataset observed in the current study was the same as that found in previous studies ( Yuan et al, 2020 ). The proportion of annotated functional genes was increased, and the average length of genes was markedly improved.…”
Section: Discussionsupporting
confidence: 88%
“…Full-length transcriptomes of the two grasshoppers Gomphocerus licenti and Mongolotettix japonicus have been published previously, which yielded 590,112 and 566,165 circular consensus sequences (CCSs) as well as 458,131 and 428,979 full-length non-chimeric (FLNC) reads, respectively. In total, 17,970 and 16,766 unigenes were identified, with 17,495 and 16,373 coding sequences (CDSs), 1,082 and 813 transcription factors (TFs), 11,840 and 10,814 simple sequence repeats (SSRs), and 905 and 706 long non-coding RNAs (lncRNAs) by analyzing the transcriptomes of G. licenti and M. japonicus , respectively; 15,803 and 14,846 respective unigenes were annotated in public databases ( Yuan et al, 2020 ).…”
Section: Introductionmentioning
confidence: 99%
“…Our 88.8% ORP BUSCO completion score was similar to or higher than those seen in recently published insect transcriptome data (e.g., [ 60 62 ] . ).…”
Section: Discussionsupporting
confidence: 87%
“…Our 88.8% ORP BUSCO completion score was similar to or higher than those seen in recently published insect transcriptome data (e.g., [60][61][62].). Likewise, our ORP TransRate assembly score of 0.215 is within the range of scores currently reported for insect transcriptome data (e.g., [63,64]), and is of higher quality than nearly 50% of transcriptomes deposited in the NCBI TSA database as of 2016 [47].…”
Section: Orp Assembly Quality Is Comparable To Assemblies Of Non-mode...supporting
confidence: 86%