Characterization and antagonistic effect of isolated Trichoderma sp. against pathogens under Clusterbean (Cyamopsis tetragonoloba L.)

Abstract

A green house experiment was conducted at Department of Plant Pathology, College of Agriculture, Bikaner, to characterize the eight isolates of Trichoderma sp. ( TH-8, TH-SOG, TH-HMG, TH-BKN, TH-SIK, TH-DUP, TH-JOB and TH-JAI) were collected from different locations of clusterbean (Cyamopsis tetragonoloba L.) fields in western Rajasthan and tested for their antagonistic property against soil borne plant pathogens. It was further studied for morphological and physiological characteristics such as color of mycelium, pigmentation of substrate, type, amount of mycelium growth, effect of temperature and pH. It was observed that, Potato Dextrose Agar (PDA) media, temp range 25-30 0 C and pH 7.0-7.5 were found most effective for optimum growth and sporulation of TH-8. The isolate Trichoderma harzianum-8 (isolated from SKRAU Bikaner, Rajasthan ) was found best up to 70 per cent of inhibition of mycelial growth of different clusterbean pathogen viz., Macrophomina phaseolina, Fusarium oxysporum, Sclerotinia sclerotiorum .

Morpho-cultural and molecular characterization of Trichoderma species from apple rhizosphere of North-western Himalayan temperate region of India

“…Similarly, based on colony shape, 4 groups were formed namely Group I, II, III and IV accommodating 16.67, 16.67, 25 and 41.66 per cent isolates, respectively. Present results were in close agreement with the observations of different workers around the world [30,31,32] who also found that the colonies of various Trichoderma isolates were at cottony, uffy or raised and at granular in texture having regular or irregular margins on PDA medium. Sharma and Singh [33] also reported that the majority of Trichoderma isolates showed different colony colour like dark green (12 isolates), whitish green (9 isolates), light green (6 isolates) and rest of the isolates (3) had yellowish green colonies.…”

“…The highest mycelial dry weight was recorded in PR2 isolate and the least in PTi3 isolate. A signi cant variation in terms of mycelial dry weight of different isolates of Trichoderma was also reported by different workers[32,49,50,51,52].…”

Influence of Potassium and Zinc on Growth, Yield and Economics of Cluster Bean (Cyamopsis tetragonoloba L.)

“…Further increase in dry weight observed with the application of zinc (20 kg/ha) might be due to Zinc application creating a balanced nutritional environment which enhanced metabolic activities and photosynthetic rate, resulting in improvement and ultimately accumulation of plant dry matter. Similar types of results were reported by Meena et al [11].…”

Impact of Agrochemicals on Soil Microbiota and Management: A Review

“…The most commonly used biopesticides include Bacillus thuringiensis (Bt), Baculo viruses, Trichoderma, Azadirachta indica. PIPs, for example, Bacillus thuringiensis is the most globally popular, which is being used against moth larvae on plants, and the strains are made specifically for the larvae of mosquitoes and flies [121]. Important among microbial biopesticides are: (1) the Baculo viruses to target specific viruses which exterminates the disease-causing to lepidopterous insects of cotton, rice, and vegetables [122], 2Trichoderma and Trichoderma-based products being effective against soil-borne diseases (i.e., root rot) and control of rots and wilts in dryland crops such as black gram (Vigna mungo), groundnut (Arachis hypogaea), chickpea (Cicer arietinum) and green gram (Vigna radiate) [120] and (3) the entomopathogenic nematodes (EPNs) of the genera Heterorhabditis sp.…”