Characterization and antimicrobial mechanism of CF-14, a new antimicrobial peptide from the epidermal mucus of catfish

Li, Tingting; Liu, Quanwei; Wang, Dangfeng; Lǐ, Jiànróng

doi:10.1016/j.fsi.2019.07.015

Cited by 51 publications

(28 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As the key component in the innate immunity, AMPs are generated in the sites where the body is most vulnerable to pathogen invasion. In mammals, the AMPs-rich mucus resists the colonization of parasites, bacteria and fungi [ 74 , 75 ]. AMPs are also found in phagocytic granulocytes and mast cells [ 76 ].…”

Section: Natural Distribution Of Ampsmentioning

confidence: 99%

Antimicrobial peptides: mechanism of action, activity and clinical potential

et al. 2021

View full text Add to dashboard Cite

The management of bacterial infections is becoming a major clinical challenge due to the rapid evolution of antibiotic resistant bacteria. As an excellent candidate to overcome antibiotic resistance, antimicrobial peptides (AMPs) that are produced from the synthetic and natural sources demonstrate a broad-spectrum antimicrobial activity with the high specificity and low toxicity. These peptides possess distinctive structures and functions by employing sophisticated mechanisms of action. This comprehensive review provides a broad overview of AMPs from the origin, structural characteristics, mechanisms of action, biological activities to clinical applications. We finally discuss the strategies to optimize and develop AMP-based treatment as the potential antimicrobial and anticancer therapeutics.

show abstract

Section: Natural Distribution Of Ampsmentioning

confidence: 99%

Antimicrobial peptides: mechanism of action, activity and clinical potential

et al. 2021

View full text Add to dashboard Cite

show abstract

“…In contrast to CNC-4, however, YD1 was able to penetrate bacterial membranes without the formation of disruptive pores ( Rahman et al, 2017 ). Similarly, CF-14, an antimicrobial peptide isolated from catfish epidermal mucus, was able to access the inside of bacterial cells and bind DNA ( Li et al, 2019 ). Lastly, the cell-penetrating synthetic antimicrobial peptide analog P7 was shown to bind bacterial DNA and inhibit cell division ( Li et al, 2015 ).…”

Section: Discussionmentioning

confidence: 99%

A nematode-derived, mitochondrial stress signaling-regulated peptide exhibits broad antibacterial activity

et al. 2021

View full text Add to dashboard Cite

A dramatic rise of infections with antibiotic-resistant bacterial pathogens continues to challenge the healthcare field due to the lack of effective treatment regimes. As such, there is an urgent need to develop new antimicrobial agents that can combat these multidrug-resistant superbugs. Mitochondria are central regulators of metabolism and other cellular functions, including the regulation of innate immunity pathways involved in the defense against infection. The mitochondrial unfolded protein response (UPRmt) is a stress-activated pathway that mitigates mitochondrial dysfunction through the regulation of genes that promote recovery of the organelle. In the model organism Caenorhabditis elegans, the UPRmt also mediates an antibacterial defense program that combats pathogen infection, which promotes host survival. We sought to identify and characterize antimicrobial effectors that are regulated during the UPRmt. From our search, we discovered that the antimicrobial peptide CNC-4 is upregulated during this stress response. CNC-4 belongs to the caenacin family of antimicrobial peptides, which are predominantly found in nematodes and are known to have anti-fungal properties. Here, we find that CNC-4 also possesses potent antimicrobial activity against a spectrum of bacterial species and report on its characterization.

show abstract

“…It has been reported that a defensin from ducks named AvBD2 is highly stable in temperatures ranging from 20 to 100°C and a pH range from 3.0 to 12.0 (Ma et al., 2009). Moreover, CF‐14 peptide could remain bioactive stable at pH values ranging from 4.0 to 12.0 and temperatures ranging from 40 to 80°C (Li, Liu, et al., 2019).…”

Section: Discussionmentioning

confidence: 99%

Application of antimicrobial peptide Mytichitin‐A in pork preservation during refrigerated storage

Meng

Yang

Sun

et al. 2021

J Food Process Preserv

View full text Add to dashboard Cite

Antimicrobial peptide Mytichitin-A has been expressed in large quantities in Pichia pastoris and has good antibacterial activity against Gram-positive bacteria. To explore its potential as a food preservative in a pork system, the activity of P. pastoris-derived Mytichitin-A against Pseudomonas spp., one of dominant spoilage flora in meat products, was first conducted. Results showed that Mytichitin-A effectively inhibited the growth of both P. fluorescens and P. tolaasii. Moreover, it exhibited a broad range of pH and thermal stability, was resistant to various protease digestion and amino acid interferences, and had very low hemolytic activity. Importantly, 25 μg/ml Mytichitin-A could effectively inhibit the bacterial growth, the increase of drip loss, pH value, lipid oxidation, and total volatile alkaline nitrogen during pork storage. Thus, it was conducive to the maintenance of pork quality. These results suggested that P. pastorisderived Mytichitin-A has excellent potential as a preservative for fresh pork. Practical applicationsThis research reveals that Mytichitin-A has a great potential for the fresh pork preservation during storage and could conducive to the maintenance of pork quality. The completion of this study also contributes to the development of novel preservation method to maintain meat quality in the future. How to cite this article: Meng D-M, Yang X-M, Sun X-Q, Cheng L, Fan Z-C. Application of antimicrobial peptide Mytichitin-A in pork preservation during refrigerated storage. J

show abstract

Characterization and antimicrobial mechanism of CF-14, a new antimicrobial peptide from the epidermal mucus of catfish

Cited by 51 publications

References 39 publications

Antimicrobial peptides: mechanism of action, activity and clinical potential

Antimicrobial peptides: mechanism of action, activity and clinical potential

A nematode-derived, mitochondrial stress signaling-regulated peptide exhibits broad antibacterial activity

Application of antimicrobial peptide Mytichitin‐A in pork preservation during refrigerated storage

Contact Info

Product

Resources

About