Characterization and antitumor activity of the extracellular carbohydrate polymer from the cyanobacterium Synechocystis ΔsigF mutant

Flores, Carlos; Lima, Raquel T.; Adessi, Alessandra; Sousa, Aureliana; Pereira, Sara B.; Granja, Pedro L.; Philippis, Roberto De; Soares, Paula; Tamagnini, Paula

doi:10.1016/j.ijbiomac.2019.06.152

Cited by 20 publications

(28 citation statements)

References 47 publications

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“…The Phormidium sp. ETS05 EPS resulted in being highly sulfated (≈13% w/w), being among the highest values found for cyanobacterial polymers [36,59]. Similar values of sulfate content were previously detected in polysaccharides produced by cyanobacteria isolated from Polynesian mats [60], in Nostoc carneum [61], or in cyanoflan, a polymer isolated from marine Cyanothece sp.…”

Section: Discussionsupporting

confidence: 78%

“…This operation was repeated three times for each sample [35]. For sulfate analysis, the lyophilized EPS were hydrolyzed in 2 M HCl at 100 • C for 2 h, centrifuged after cooling, and the supernatant was analyzed by ion-exchange chromatography [36].…”

Section: Eps Characterizationmentioning

confidence: 99%

“…For sulfate analysis, the system was equipped with a continuously regenerated anion-trap column (USA), a continuous anionic self-regenerating suppressor, a conductivity detector (ED50), an IonPac PA11 4 × 250 mm column (Dionex, Sunnyvale, CA, USA), and a reagent-free Dionex system producing high-purity 50 mM KOH at a flow rate of 2 mL/min. Sulfate solutions (1 to 10 mg/L; Fluka, Switzerland) were used as standards [36].…”

Section: Eps Characterizationmentioning

confidence: 99%

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Anti-Inflammatory Activity of Exopolysaccharides from Phormidium sp. ETS05, the Most Abundant Cyanobacterium of the Therapeutic Euganean Thermal Muds, Using the Zebrafish Model

et al. 2020

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The Euganean Thermal District (Italy) represents the oldest and largest thermal center in Europe, and its therapeutic mud is considered a unique product whose beneficial effects have been documented since Ancient Roman times. Mud properties depend on the heat and electrolytes of the thermal water, as well as on the bioactive molecules produced by its biotic component, mainly represented by cyanobacteria. The investigation of the healing effects of compounds produced by the Euganean cyanobacteria represents an important goal for scientific validation of Euganean mud therapies and for the discovering of new health beneficial biomolecules. In this work, we evaluated the therapeutic potential of exopolysaccharides (EPS) produced by Phormidium sp. ETS05, the most abundant cyanobacterium of the Euganean mud. Specifically, Phormidium EPS resulted in exerting anti-inflammatory and pro-resolution activities in chemical and injury-induced zebrafish inflammation models as demonstrated using specific transgenic zebrafish lines and morphometric and expression analyses. Moreover, in vivo and in vitro tests showed no toxicity at all for the EPS concentrations tested. The results suggest that these EPS, with their combined anti-inflammatory and pro-resolution activities, could be one of the most important therapeutic molecules present in the Euganean mud and confirm the potential of these treatments for chronic inflammatory disease recovery.

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Section: Discussionsupporting

confidence: 78%

Section: Eps Characterizationmentioning

confidence: 99%

Section: Eps Characterizationmentioning

confidence: 99%

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Anti-Inflammatory Activity of Exopolysaccharides from Phormidium sp. ETS05, the Most Abundant Cyanobacterium of the Therapeutic Euganean Thermal Muds, Using the Zebrafish Model

et al. 2020

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“…The apparent molecular mass of cyanoflan was determined according to a previously reported method (Flores et al, 2019). The samples concentration was 3 mg mL -1 and as standards dextran (Sigma-Aldrich, USA) with different molecular weights (2000 kDa, 1100 kDa, 410 kDa, 150 kDa and 50 kDa) and sucrose (340 Da) were used.…”

Section: J O U R N a L P R E -P R O O Fmentioning

confidence: 99%

“…The polysaccharides produced by several strains of Nostoc have a single fraction (Han et al, 2014;Li et al, 2018), while the ones from Synechocystis sp. PCC 6803 and Cyanospira capsulata have several different fractions (Flores et al, 2019;Garozzo, Impallomeni, Spina, & Sturiale, 1998).…”

Section: Glycosidic-composition Of Cyanoflanmentioning

confidence: 99%

Cyanoflan: A cyanobacterial sulfated carbohydrate polymer with emulsifying properties

Mota

Vidal

Pandeirada

et al. 2020

Carbohydrate Polymers

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This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

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Cyanobacterial Extracellular Polymeric Substances (EPS)

Mota¹,

Flores²,

Tamagnini³

2021

Polysaccharides of Microbial Origin

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Characterization and antitumor activity of the extracellular carbohydrate polymer from the cyanobacterium Synechocystis ΔsigF mutant

Cited by 20 publications

References 47 publications

Anti-Inflammatory Activity of Exopolysaccharides from Phormidium sp. ETS05, the Most Abundant Cyanobacterium of the Therapeutic Euganean Thermal Muds, Using the Zebrafish Model

Anti-Inflammatory Activity of Exopolysaccharides from Phormidium sp. ETS05, the Most Abundant Cyanobacterium of the Therapeutic Euganean Thermal Muds, Using the Zebrafish Model

Cyanoflan: A cyanobacterial sulfated carbohydrate polymer with emulsifying properties

Cyanobacterial Extracellular Polymeric Substances (EPS)

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