Characterization and Application of Biosurfactant Produced by Bacillus licheniformis R2

Joshi, Sanket; Subramanian, Geetha; Desai, Anjana J.

doi:10.1007/s12010-015-1746-4

Cited by 66 publications

(44 citation statements)

References 31 publications

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“…IFT and ST reduction are reported to have an important effect on light or heavy crude oil recovery by bacterial biosurfactants under laboratory experiments of core-flood or sand-pack column studies (Al-Sulaimani et al 2012;Joshi and Desai 2013;Joshi et al 2015). In this study, there was no much reduction in ST and IFT between treated samples and controls were observed, and only minor reduction in ST from day 17 and day 21 was observed.…”

Section: Discussionmentioning

confidence: 42%

Potential in heavy oil biodegradation via enrichment of spore forming bacterial consortia

Al-Bahry

Al-Wahaibi

Al-Hinai

et al. 2016

J Petrol Explor Prod Technol

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It is quite challenging to recover heavy crude oil due to its high viscosity and long chain hydrocarbon contents. In this study the isolation of thermophilic bacterial consortia from crude oil contaminated soil samples and their ability to degrade heavy crude oil were investigated. Thermophilic spore forming bacteria were inoculated with heavy oil in minimal salt media. Maximum growth was observed on 21st day of incubation at 40°C. Samples were analyzed for biosurfactant production, where a substantial decrease in surface tension and interfacial tension was not observed. A well-assay was used to check the potential of bacterial consortia for oil-clearing zones on oil agar plate with bacterial cultures and their cell-free filtrates. 16S rRNA sequencing of 27 isolates from oil contaminated soil showed that isolates belonged to three classes: Alphaproteobacteria, gamma-proteobacteria and Bacilli. Scanning electron microscopy showed that the oil utilizing bacteria were rod-shaped with rough surface and fimbria. Gas chromatography analysis of treated oil after 21 days showed that bacterial consortia efficiently degraded heavy crude oil to light hydrocarbons ranging from C11-C27, from initial C37? carbon chain of untreated controls. These bacterial consortia showed potential in heavy crude oil biodegradation by breaking it down to smaller hydrocarbon composition.

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Section: Discussionmentioning

confidence: 42%

Potential in heavy oil biodegradation via enrichment of spore forming bacterial consortia

Al-Bahry

Al-Wahaibi

Al-Hinai

et al. 2016

J Petrol Explor Prod Technol

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“…The mass spectra ( Figure 4B ) revealed the major group of peaks at m/z values between 1000 and 1090 (The mass spectra of remaining bands are as Supplementary Figure S1). This group could be attributed to the different variants of surfactins or lichenysins, as previously described (Pereira et al, 2013; Al-Wahaibi et al, 2014; Joshi et al, 2015). The HPTLC–ESI–MS is quite easy and quick technique to identify the biosurfactants.…”

Section: Resultsmentioning

confidence: 54%

“…Considering the molecular mass of different homologs of lichenysin (C 12 –C 16 ), these peaks were identified as protonated ions [M + H] + m/z 1049.1; sodium adduct ions [M + Na] + with m/z of 1015.5, 1029.5, 1043.5, and 1057.6; sodium adducts [M-H + 2Na] + with m/z of 1051.5, 1065.5, and 1079.5; potassium adducts [M + K] + with m/z of 1087.5. MALDI-TOF analysis of W16 biosurfactant showed similarity with lichenysin-A, produced by B. licheniformis strains (Grangemard et al, 1999; Mikkola et al, 2000; Li et al, 2008; Zhang et al, 2014; Joshi et al, 2015). They have reported that lichenysin – A is a cyclic heptalipopeptide having a small peptide (Gln, Leu, Leu, Val, Asp, Leu, and Ile) linked to 3-hydroxy fatty acid residue with amide (Gln) and lactone (Ile) bonds forming a cyclic structure, with main fatty acids are 3-hydroxylated tri, tetra, penta, and hexadecanoic acids.…”

Section: Resultsmentioning

confidence: 91%

“…The IR spectrum in KBr showed bands characteristic of peptides at 3300–3400 cm −1 (N-H stretching mode) and at 1650–1700 cm −1 (stretching mode of the CO–N bond). The bands at 1200–1400 cm −1 reflected the aliphatic chains (–CH 3 , –CH 2 –) of the isolated fraction (Al-Sulaimani et al, 2011a; Al-Wahaibi et al, 2014; Joshi et al, 2015). These results imply the presence of aliphatic groups as well as a peptide-like moiety in the biosurfactant.…”

Section: Resultsmentioning

confidence: 99%

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Production, Characterization, and Application of Bacillus licheniformis W16 Biosurfactant in Enhancing Oil Recovery

et al. 2016

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The biosurfactant production by Bacillus licheniformis W16 and evaluation of biosurfactant based enhanced oil recovery (EOR) using core-flood under reservoir conditions were investigated. Previously reported nine different production media were screened for biosurfactant production, and two were further optimized with different carbon sources (glucose, sucrose, starch, cane molasses, or date molasses), as well as the strain was screened for biosurfactant production during the growth in different media. The biosurfactant reduced the surface tension and interfacial tension to 24.33 ± 0.57 mN m−1 and 2.47 ± 0.32 mN m−1 respectively within 72 h, at 40°C, and also altered the wettability of a hydrophobic surface by changing the contact angle from 55.67 ± 1.6 to 19.54°± 0.96°. The critical micelle dilution values of 4X were observed. The biosurfactants were characterized by different analytical techniques and identified as lipopeptide, similar to lichenysin-A. The biosurfactant was stable over wide range of extreme environmental conditions. The core flood experiments showed that the biosurfactant was able to enhance the oil recovery by 24–26% over residual oil saturation (Sor). The results highlight the potential application of lipopeptide biosurfactant in wettability alteration and microbial EOR processes.

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“…JF-2, was reported by Thomas et al (1993). Joshi et al (2015) reported additional 37.1 % of heavy oil from Berea sandstone cores at 80°C was achieved using a lipopeptide-type of biosurfactant. Previous studies reported that biosurfactants could potentially be used in conjunction with synthetic surfactants to provide more cost-effective enhanced oil recovery and subsurface remediation (Youssef et al 2007a, b).…”

Section: Coreflood Experiments Using Chemical Surfactant and Biosurfamentioning

confidence: 92%

Injection of biosurfactant and chemical surfactant following hot water injection to enhance heavy oil recovery

et al. 2015

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This study investigates the potential of enhancing oil recovery from a Middle East heavy oil field via hot water injection followed by injection of a chemical surfactant and/or a biosurfactant produced by a Bacillus subtilis strain which was isolated from oil-contaminated soil. The results reveal that the biosurfactant and the chemical surfactant reduced the residual oil saturation after a hot water flood. Moreover, it was found that the performance of the biosurfactant increased by mixing it with the chemical surfactant. It is expected that the structure of the biosurfactant used in this study was changed when mixed with the chemical surfactant as a probable synergetic effect of biosurfactant-chemical surfactants was observed on enhancing oil recovery, when used as a mixture, rather than alone. This work proved that it is more feasible to inject the biosurfactant as a blend with the chemical surfactant, at the tertiary recovery stage. This might be attributed to the fact that in the secondary mode, improvement of the macroscopic sweep efficiency is important, whereas in the tertiary recovery mode, the microscopic sweep efficiency matters mainly and it is improved by the biosurfactantchemical surfactant mixture. Also as evidenced by this study, the biosurfactant worked better than the chemical surfactant in reducing the residual heavy oil saturation after a hot water flood.

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Characterization and Application of Biosurfactant Produced by Bacillus licheniformis R2

Cited by 66 publications

References 31 publications

Potential in heavy oil biodegradation via enrichment of spore forming bacterial consortia

Potential in heavy oil biodegradation via enrichment of spore forming bacterial consortia

Production, Characterization, and Application of Bacillus licheniformis W16 Biosurfactant in Enhancing Oil Recovery

Injection of biosurfactant and chemical surfactant following hot water injection to enhance heavy oil recovery

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