2014
DOI: 10.1016/j.jbiomech.2014.09.035
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Characterization and assessment of hyperelastic and elastic properties of decellularized human adipose tissues

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Cited by 61 publications
(54 citation statements)
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“…The 5 µm spherical AFM tip enabled indentation testing over a larger region of the coatings, providing an analysis of the properties on the scale of individual cells. Notably, the α‐amylase‐digested DAT coatings were stiffer than the previous values measured through bulk compression testing for the intact DAT and α‐amylase‐digested DAT foams, which were in the range of ≈2–4 kPa and similar to the values reported for native human fat . The increased stiffness of the coatings may be related to the difference in the scale of the testing methods employed, as well as structural differences in the materials and their processing to form coatings.…”
Section: Discussionmentioning
confidence: 46%
“…The 5 µm spherical AFM tip enabled indentation testing over a larger region of the coatings, providing an analysis of the properties on the scale of individual cells. Notably, the α‐amylase‐digested DAT coatings were stiffer than the previous values measured through bulk compression testing for the intact DAT and α‐amylase‐digested DAT foams, which were in the range of ≈2–4 kPa and similar to the values reported for native human fat . The increased stiffness of the coatings may be related to the difference in the scale of the testing methods employed, as well as structural differences in the materials and their processing to form coatings.…”
Section: Discussionmentioning
confidence: 46%
“…The methodology for decellularizing adipose tissue was pioneered by Flynn and colleagues [ 61 , 62 , 63 , 64 , 65 , 66 , 67 , 68 , 69 , 70 , 71 , 72 , 73 ] and has since been confirmed by multiple independent laboratories [ 74 , 75 , 76 , 77 , 78 , 79 , 80 , 81 , 82 ]. These methods combine biological (enzyme digestion), chemical, and physical processing steps to achieve the uniform and consistent manufacture of protein scaffolds depleted of contaminating genomic DNA and lipids.…”
Section: Extracellular Matrix (Ecm) From Decellularized Adipose Timentioning
confidence: 99%
“…Through the analysis of published decellularization methods, the physical steps included repeated freeze‐thaw, homogenization, mechanical massaging, stirring or high‐pressure treatment. Among them, three cycles of freeze‐thaw, from −80°C to 37°C, was first used by Flynn in the process of adipose decellularization, which was the most widely adopted method by researchers . This technique could minimize the amounts of chemical agents required for effective decellularization.…”
Section: Preparation Of Datmentioning
confidence: 99%