Characterization and bioactivities of exopolysaccharide produced from probiotic Lactobacillus plantarum 47FE and Lactobacillus pentosus 68FE.

Saif, Feriala A.A. Abo; Sakr, Ebtehag A.E.

doi:10.1016/j.bcdf.2020.100231

Cited by 25 publications

(9 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Several authors have reported the isolation of other Lpb. plantarum strains from various foods and their safety due to the absence of acquired antibiotic resistance genes [39,40] and virulence factors [39,41]. The identification and characterization of Lpb.…”

Section: Discussionmentioning

confidence: 99%

Characterization of a Lactiplantibacillus plantarum R23 Isolated from Arugula by Whole-Genome Sequencing and Its Bacteriocin Production Ability

Barbosa

Albano

Silva³

et al. 2021

IJERPH

View full text Add to dashboard Cite

Lactiplantibacillus plantarum is one of the lactic acid bacteria species most used as probiotics and starter cultures in food production. Bacteriocin-producers Lpb. plantarum are also promising natural food preservatives. This study aimed to characterize Lpb. plantarum R23 and its bacteriocins (R23 bacteriocins). The genome sequence of Lpb. plantarum R23 was obtained by whole-genome sequencing (WGS) in an Illumina NovaSeq platform. The activity of Lpb. plantarum R23-produced bacteriocin against two Listeria monocytogenes strains (L7946 and L7947) was evaluated, and its molecular size was determined by tricine-SDS-PAGE. No virulence or antibiotic resistance genes were detected. Four 100% identical proteins to the class II bacteriocins (Plantaricin E, Plantaricin F, Pediocin PA-1 (Pediocin AcH), and Coagulin A) were found by WGS analysis. The small (<6.5 kDa) R23 bacteriocins were stable at different pH values (ranging from 2 to 8), temperatures (between 4 and 100 °C), detergents (all, except Triton X-100 and Triton X-114 at 0.01 g/mL), and enzymes (catalase and α-amylase), did not adsorb to the producer cells, had a bacteriostatic mode of action and their maximum activity (AU/mL = 12,800) against two L. monocytogenes strains occurred between 15 and 21 h of Lpb. plantarum R23 growth. Lactiplantibacillus plantarum R23 showed to be a promising bio-preservative culture because, besides being safe, it produces a stable bacteriocin or bacteriocins (harbors genes encoding for the production of four) inhibiting pathogens as L. monocytogenes. Further studies in different food matrices are required to confirm this hypothesis and its suitability as a future starter culture.

show abstract

Section: Discussionmentioning

confidence: 99%

Characterization of a Lactiplantibacillus plantarum R23 Isolated from Arugula by Whole-Genome Sequencing and Its Bacteriocin Production Ability

Barbosa

Albano

Silva³

et al. 2021

IJERPH

View full text Add to dashboard Cite

show abstract

“…The sulphated sites and stereochemistry of EPSs activate HC II according to the allosteric mechanism [50] . One study proved that EPS47FE and EPS68FE which are secreted by L. plantarum 47FE and Lactiplantibacillus pentosus 68FE, respectively, exhibit strong anticoagulant and fibrinolytic activity [51] .…”

Section: The Physiological Functions Of Epssmentioning

confidence: 99%

Exopolysaccharide production by lactic acid bacteria: the manipulation of environmental stresses for industrial applications

Nguyen¹,

Nguyen²,

Bui³

et al. 2020

AIMS Microbiology

136

View full text Add to dashboard Cite

Exopolysaccharides (EPSs) are biological polymers secreted by microorganisms including Lactic acid bacteria (LAB) to cope with harsh environmental conditions. EPSs are one of the main components involved in the formation of extracellular biofilm matrix to protect microorganisms from adverse factors such as temperature, pH, antibiotics, host immune defenses, etc.. In this review, we discuss EPS biosynthesis; the role of EPSs in LAB stress tolerance; the impact of environmental stresses on EPS production and on the expression of genes involved in EPS synthesis. The evaluation results indicated that environmental stresses can alter EPS biosynthesis in LAB. For further studies, environmental stresses may be used to generate a new EPS type with high biological activity for industrial applications.

show abstract

“…EPS-1 had a significantly higher molecular weight (Mw) of 2613.4 kDa compared to EPS-2 with a MW of 93.4kDa, suggesting that a single strain has the ability to produce distinct exopolysaccharides with significantly different MWs [120] . Lactobacillus plantarum isolated from Egyptian cheese products produced the highest EPS yield of 11.86g/L compared with other Lactobacillus strains, indicating that EPS production varies among strains, even when obtained from the same origin [121] . Extraction conditions have a significant impact on yield, MW and composition [122] .…”

Section: Extraction Methodsmentioning

confidence: 94%

Bacterial Exopolysaccharides: From Production to Functional Features

Talbi,

Elmarrakchy,

Youssfi

et al. 2023

Prog Micobes Mol Biol

View full text Add to dashboard Cite

Exopolysaccharides, known as bacterial EPS, are complex sugar polymers that bacteria secrete into their environment. EPSs play a crucial role in bacterial survival and proliferation by protecting cells from environmental threats. They also contribute to proper adhesion to numerous surfaces through biofilm production. Bacterial EPS display a wide range of biological activities conferred by their outstanding physical and chemical properties, which make them great candidates for medical and industrial applications. The main biological activities recorded up-to-day include antioxidant, anticancer, antiviral, anti-inflammatory, anticancer, antibacterial, immune-modulatory and chelating properties. They also can be used as thickening agents in the industry or as additives to improve soil quality in agriculture. The current review offers a thorough overview of bacterial EPS, their biosynthesis processes and regulation, and biotechnological strategies to increase their production. The review also unravels the main extraction, purification, and identification techniques and highlights the key functional features of these complex molecules.

show abstract

Characterization and bioactivities of exopolysaccharide produced from probiotic Lactobacillus plantarum 47FE and Lactobacillus pentosus 68FE.

Cited by 25 publications

References 50 publications

Characterization of a Lactiplantibacillus plantarum R23 Isolated from Arugula by Whole-Genome Sequencing and Its Bacteriocin Production Ability

Characterization of a Lactiplantibacillus plantarum R23 Isolated from Arugula by Whole-Genome Sequencing and Its Bacteriocin Production Ability

Exopolysaccharide production by lactic acid bacteria: the manipulation of environmental stresses for industrial applications

Bacterial Exopolysaccharides: From Production to Functional Features

Contact Info

Product

Resources

About