Characterization and bioactivities of the exopolysaccharide from a probiotic strain of Lactobacillus plantarum WLPL04

Abstract

Exopolysaccharide (EPS) was extracted and purified from Lactobacillus plantarum WLPL04, which has been confirmed previously as a potential probiotic for its antagonistic and immune-modulating activity. It has a molecular weight of 6.61 × 10 Da, consisting of xylose, glucose, and galactose in an approximate molar ratio of 3.4:1.8:1. Microstructural studies demonstrated that the EPS appeared as a smooth sheet structure with many homogeneous rod-shaped lumps. The preliminary in vitro assays indicated that the EPS could significantly inhibit the adhesion of Escherichia coli O157:H7 to HT-29 cells in competition, replacement, and inhibition assays at a dose of 1.0 mg/mL, with an inhibition rate of 20.24 ± 2.23, 29.71 ± 1.21, and 30.57 ± 1.73%, respectively. Additionally, the EPS exhibited strong inhibition against biofilm formation by pathogenic bacteria, including Pseudomonas aeruginosa CMCC10104, E. coli O157:H7, Salmonella Typhimurium ATCC13311, and Staphylococcus aureus CMCC26003. Furthermore, the EPS showed good inhibitory activity against the proliferation of HT-29 cells. The characteristics and bioactivities of this EPS may make it a promising candidate in developing functional food.

Exopolysaccharides produced by Lactobacillus strains suppress HT‑29 cell growth via induction of G0/G1 cell cycle arrest and apoptosis

“…Starter culture was prepared as previously described of inoculum, then batch fermentation was performed in a 5.0L capacity fermentor (Biotech-2002; Bao Xing Bio-Engineering Equipment Co., Ltd, Shanghai, China) at 37°C with an inoculum concentration of 3.0% to obtain the fermented broth ( 15 ). Extracellular polymeric substances were obtained as described previously ( 34 ). The crude EPS fractions were separated and purified according to procedures described by Lin et al with slight modifications ( 39 ).…”

Ability of a Wild Weissella Strain to Modify Viscosity of Fermented Milk

“…Total EPS from the SDM medium were isolated by means of double cold ethanol precipitation method without dialysis (Rimada and Abraham, 2003). The absence of protein and nucleic acids in the EPS was assessed by spectrophotometric analysis using a UV-visible spectrophotometer (Jasco V-530), at a wavelength of 260-280 nm (Liu et al, 2016).…”

Genetic and Biochemical Characterization of an Exopolysaccharide With in vitro Antitumoral Activity Produced by Lactobacillus fermentum YL-11

“…It was suggested that EPS from L. acidophilus 10307 could induce cytotoxicity in two colon cancer cell lines, HCT15 and Caco2 cells (Deepak et al, 2016). Additionally, EPS from L. plantarum WLPL04 and L. plantarum YW32 showed significant inhibitory effects on HT-29 colon cancer cells (Wang et al, 2015b;Liu et al, 2017); EPS from L. plantarum RJF 4 displayed antitumoral activity in pancreatic cancer cells (Dilna et al, 2015). cell-bound EPS from L. rhamnosus ATCC 9595 could inhibit the growth of PANC1 and HT-29 cancer cells (Kim et al, 2006).…”