Characterization and biocontrol efficacy of lytic phage (KPP-1) that infects multidrug resistant Klebsiella variicola

Nikapitiya, Chamilani; Chandrarathna, H.P.S.U.; Dias, Mawalle Kankanamge Hasitha Madhawa; Lee, Jehee; Zoysa, Mahanama De

doi:10.1007/s42770-023-01037-8

Braz J Microbiol

2023

DOI: 10.1007/s42770-023-01037-8

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Characterization and biocontrol efficacy of lytic phage (KPP-1) that infects multidrug resistant Klebsiella variicola

Chamilani Nikapitiya

H.P.S.U. Chandrarathna

Mawalle Kankanamge Hasitha Madhawa Dias

et al.

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2024

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Hypermucoviscous Multidrug-Resistant Klebsiella variicola Strain LL2208 Isolated from Chinese Longsnout Catfish (Leiocassis longirostris): Highly Similar to Human K. variicola Strains

Li,

Yu,

et al. 2024

Pathogens

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Outbreaks of bacterial diseases occur in farmed Chinese longsnout catfish (Leiocassis longirostris). Due to limited information on aquatic Klebsiella variicola-infected animals, this study aimed to identify strain LL2208 isolated from diseased L. longirostris, determine its biological features, and evaluate its risk to public health. Strain LL2208 was tested for molecular identification, challenge, string, biofilm formation, and antimicrobial susceptibility. Furthermore, the whole genome of the strain was sequenced and analyzed. Based on molecular identification, strain LL2208 was identified as K. variicola. Artificial infection showed that this strain was moderately virulent to L. longirostris with an LD50 = 7.92 × 107 CFU/mL. Antibiotic sensitivity tests showed that this strain was resistant to penicillins, macrolides, aminoglycosides, amphenicols, glycopeptides, and lincosamide, indicating multidrug resistance. Strain LL2208 has a genome size of 5,557,050 bp, with a GC content of 57.38%, harboring 30 antimicrobial resistance genes and numerous virulence-related genes. Its molecular type was ST595-KL16-O5. Collinearity analysis showed that strain LL2208 was highly similar to the human-derived K. variicola strain. In conclusion, the multidrug-resistant and virulent K. variicola strain LL2208 was isolated from fish and may have originated from humans. These results provide a foundation for further studies on the transmission of K. variicola between humans and aquatic animals.

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Hypermucoviscous Multidrug-Resistant Klebsiella variicola Strain LL2208 Isolated from Chinese Longsnout Catfish (Leiocassis longirostris): Highly Similar to Human K. variicola Strains

Li,

Yu,

et al. 2024

Pathogens

View full text Add to dashboard Cite

show abstract

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Characterization and biocontrol efficacy of lytic phage (KPP-1) that infects multidrug resistant Klebsiella variicola

Cited by 1 publication

References 45 publications

Hypermucoviscous Multidrug-Resistant Klebsiella variicola Strain LL2208 Isolated from Chinese Longsnout Catfish (Leiocassis longirostris): Highly Similar to Human K. variicola Strains

Hypermucoviscous Multidrug-Resistant Klebsiella variicola Strain LL2208 Isolated from Chinese Longsnout Catfish (Leiocassis longirostris): Highly Similar to Human K. variicola Strains

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