Characterization and biodistribution of trans resveratrol-PEG-folic acid-gold nanoparticle conjugates

Abstract


 
 
 
 Purpose: To evaluate the characteristics and biodistribution of trans resveratrol-PEG-folic acid-gold nanoparticle conjugates (rsv-PEG-FA-AuNP).
 Methods: Gold nanoparticles were produced by citric reduction followed by conjugation of PEG-folic acid and resveratrol. Characterization of rsv-PEG-FA-AuNP conjugates including their particle size, zeta potential, and by Fourier transform infrared spectroscopy (FTIR), and transmission electron microscopy (TEM) was carried out. Biodistribution study of rsv-PEG-FA-AuNP was carried out using female Sprague Dawley rats. Biodistribution data were obtained from high performance liquid chromatography (HPLC) analysis.
 Results: The mean particle size and zeta potential of rsv-PEG-FA-AuNP were 249.03 ± 10.31 and - 36.33 ± 3.12 mV, respectively. TEM images showed rsv-PEG-FA-AuNP conjugates formed spherical shape. Rsv-PEG-FA-AuNP conjugates found in plasma, kidney (1.90 ± 0.20 μg/g), spleen (2.65 ± 1.18 μg/g), liver (1.74 ± 0.03 μg/g), and lung (1.82 ± 0.12 μg/g), after 90 minutes intravenous administration (i.v.) in female Sprague Dawley rats. No free resveratrol was found in plasma, kidney, or spleen after i.v administration in female dawdle Sprague Dawley rats.
 Conclusion: Resveratrol-PEG-FA-AuNP conjugates appear to be a potential chemotherapy delivery system for active targeting purposes because of its longer systemic circulation and its accumulation in the kidney.
 
 
 

Mesoporous silica nanoparticles with dual-targeting agricultural sources for enhanced cancer treatment via tritherapy

“…–OH groups were observed at 3425 cm −1 and 2931 cm −1 and Si–O–Si signals were observed at 1084 cm −1 and 950 cm −1 . 33 The addition of the –NH 2 group confirmed an additional N–H bond peak at the amine group observed at 1559; 34 after loading the drug molecule CPT, it was found that there were CO bonds, CC bonds, and C–N bonds at 1648 cm −1 , 1570 cm −1 , and 1435 cm −1 , respectively, confirming the existence of the drug 35 After the HA target, the carboxylic acid (–COOH) group was found at 1700 cm −1 ; 36 after the FA target with the N–H functional group was finally attached, the N–H characteristic peaks appeared at 1605 cm −1 and 1534 cm −1 , 37 and the FT-IR spectrum indicated that the prepared material molecules were successfully connected to rMSN-EuGd-Bi.…”