Characterization and biological activities of melanin pigment from root endophytic fungus, Phoma sp. RDSE17

Surendirakumar, Kannaiah; Pandey, R. R.; Muthukumar, Thangavelu; Sathiyaseelan, Anbazhagan; Loushambam, Surbala; Seth, Amit

doi:10.1007/s00203-022-02788-y

Cited by 12 publications

(15 citation statements)

References 51 publications

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“…The obtained results were similar to those of previous studies, which observed that filamentous fungi such as Alternaria alternata , Aspergillus oryzae , A. sydowii , A. terreus , Fusarium oxysporum , Exophiala pisciphila , Penicillium spp., Phoma sp. RDSE17, and Spissiomyces endophytica exhibited melanin production after being cultivated either on solid or liquid media ( Zhan et al, 2011 ; Pal et al, 2014 ; El-Batal and Al-Tamie, 2016 ; Suwannarach et al, 2019 ; Fernandes et al, 2021 ; Surendirakumar et al, 2022 ). Numerous studies have examined the synthesis of melanin in cultured fungal biomass of various fungal species ( Kumar et al, 2011 ; Zhan et al, 2011 ; Pal et al, 2014 ; Xu et al, 2020 ) or using submerged cultivation techniques in PDB or similar growth media ( Selvakumar et al, 2008 ; El-Naggar and El-Ewasy, 2017 ; Elsayis et al, 2022 ).…”

Section: Discussionmentioning

confidence: 99%

“…In the derma-cosmetic industry, melanin is extensively used due to its UV-protective properties commonly applied in the production of sunscreen and related products ( Choi, 2021 ; El-Naggar and Saber, 2022 ). Moreover, melanin has demonstrated impressive biological activities, including anti-cancer, antimicrobial, anti-inflammatory, and hepatoprotective properties ( El-Naggar and El-Ewasy, 2017 ; Huang et al, 2018 ; Ghadge et al, 2021 ; Surendirakumar et al, 2022 ).…”

Section: Introductionmentioning

confidence: 99%

“…In certain fungi, melanin also contributes to maintaining the structural integrity of the cell wall ( Nosanchuk et al, 2015 ). Several studies have investigated the extraction of melanin from various fungal genera, including Aspergillus , Exophiala , Gliocephalotrichum , Hortea , Inonotus , Penicillium , Phoma , Phyllosticta , Pleurotus , Schizophyllum , and Spissiomyces ( Suryanarayanan and Thennarasan, 2004 ; Selvakumar et al, 2008 ; Zhan et al, 2011 ; Jalmi et al, 2012 ; Kejžar et al, 2013 ; Liu et al, 2014 ; Pal et al, 2014 ; Arun et al, 2015 ; Hou et al, 2019 ; Suwannarach et al, 2019 ; Elsayis et al, 2022 ; Surendirakumar et al, 2022 ). However, the yield from these sources remains insufficient to meet the demands of large-scale applications.…”

Section: Introductionmentioning

confidence: 99%

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Efficient production and characterization of melanin from Thermothelomyces hinnuleus SP1, isolated from the coal mines of Chhattisgarh, India

Pandey,

Meshram,

Yehia

et al. 2024

Front. Microbiol.

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In the present study, fungi were isolated and screened from barren land in south-eastern Coalfields limited (SECL) in Chhattisgarh, India. Out of 14 isolated fungi, only three fungal isolates exhibited pigmentation in screening studies. The isolated fungal strain SP1 exhibited the highest pigmentation, which was further utilized for in vivo production, purification, and characterization of melanin pigment. The physical and chemical properties of the fungal pigment showed insolubility in organic solvents and water, solubility in alkali, precipitation in acid, and decolorization with oxidizing agents. The physiochemical characterization and analytical studies of the extracted pigment using ultraviolet–visible spectroscopy and Fourier transform infrared (FTIR) confirmed it as a melanin pigment. The melanin-producing fungus SP1 was identified as Thermothelomyces hinnuleus based on 18S-rRNA sequence analysis. Furthermore, to enhance melanin production, a response surface methodology (RSM) was employed, specifically utilizing the central composite design (CCD). This approach focused on selecting efficient growth as well as progressive yield parameters such as optimal temperature (34.4°C), pH (5.0), and trace element concentration (56.24 mg). By implementing the suggested optimal conditions, the production rate of melanin increased by 62%, resulting in a yield of 28.3 mg/100 mL, which is comparatively higher than the actual yield (17.48 ± 2.19 mg/100 mL). Thus, T. hinnuleus SP1 holds great promise as a newly isolated fungal strain that could be used for the industrial production of melanin.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Efficient production and characterization of melanin from Thermothelomyces hinnuleus SP1, isolated from the coal mines of Chhattisgarh, India

Pandey,

Meshram,

Yehia

et al. 2024

Front. Microbiol.

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“…In this study, the identification of the microorganisms present in the samples revealed a predominance of filamentous fungi of the following genera: Penicillium, Clonostachys, Phoma and Monascus. The fungi of these genera reveal the potential of species isolated from the soil of forest fragments to produce substances of biotechnological interest, such as colorants [12,15,[32][33][34].…”

Section: Discussionmentioning

confidence: 99%

Exploring colorant production by amazonian filamentous fungi: Stability and applications

Oliveira,

Sanches,

Segundo

et al. 2023

J Basic Microbiol

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The aim of this study was to investigate the production, stability and applicability of colorants produced by filamentous fungi isolated from soil samples from the Amazon. Initially, the isolates were evaluated in a screening for the production of colorants. The influences of cultivation and nutritional conditions on the production of colorants by fungal isolates were investigated. The colorants produced by selected fungal isolates were chemically characterized using the Liquid Chromatography‐Mass Spectrometry technique. The antimicrobial and cytotoxic activities, stability evaluation and applicability of the colorants were investigated. As results, we observed that the isolates Penicillium sclerotiorum P3SO224, Clonostachys rosea P2SO329 and Penicillium gravinicasei P3SO332 stood out since they produced the most intense colorants. Compounds produced by Penicillium sclerotiorum P3SO224 and Clonostachys rosea P2SO329 were identified as sclerotiorin and penicillic acid. The colorant fraction (EtOAc) produced by these species has antimicrobial activity, stability at temperature and at different pHs, stability when exposure to light and UV, and when exposed to different concentrations of salts, as well as being nontoxic and having the ability to dye fabrics and be used as a pigment in creams and soap. Considering the results found in this study, it was concluded that fungi from the soil in the Amazon have the potential to produce colorants with applications in the textile and pharmaceutical industries.

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“…For UV spectroscopic analysis, the puri ed fungal melanin and synthetic DOPA melanin were dissolved seperately in 1M KOH and its ultraviolet-visible (UV) absorption spectra was recorded in the wavelength range of 200-800 nm using Shimadzu UV-2450 Spectrophotometer (Surendirakumar et al 2022).…”

Section: Uv-spectroscopymentioning

confidence: 99%

Isolation, characterization and anti-oxidant potential of melanin pigment from Trichomerium bhatii NFCCI 4305

Suthar

Singh

2023

Preprint

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Melanin is usually formed by oxidative polymerization of phenolic compounds produced by bacteria, fungi, plants as well as animals. They have gained popularity for its multifarous properties and applications in various industries, especially the derma-cosmetic industries where fungal melanin has recently been highlighted as a potential source of natural bioactive compounds beneficial to skin and hair. The anti-oxidant potential and free radical scavenging activities of natural melanin makes them suitable for application in cosmetic products. In the present we have isolated and characterized a melanin pigment from Trichomerium bhatii NFCCI 4305, an unconventional fungus of Indian origin. The biological properties of purified melanin from the fungus were evaluated for its antioxidant activity. Following alkali acid treatment of the fermented broth, the pigment was extracted and further, its nature was confirmed as melanin through physico-chemical tests and spectral (UV and FTIR) analysis. The analysis of the elemental composition indicated that the pigment fall into DOPA category of melanin. The purified melanin showed a good DPPH (1, 1-Diphenyl-2-picrylhydrazyl) free radical-scavenging activity. To our understanding this is the first report on melanin characterization from the fungal genus Trichomerium.

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Characterization and biological activities of melanin pigment from root endophytic fungus, Phoma sp. RDSE17

Cited by 12 publications

References 51 publications

Efficient production and characterization of melanin from Thermothelomyces hinnuleus SP1, isolated from the coal mines of Chhattisgarh, India

Efficient production and characterization of melanin from Thermothelomyces hinnuleus SP1, isolated from the coal mines of Chhattisgarh, India

Exploring colorant production by amazonian filamentous fungi: Stability and applications

Isolation, characterization and anti-oxidant potential of melanin pigment from Trichomerium bhatii NFCCI 4305

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