Characterization and Biological Activities of Silver Nanoparticles Synthesized from Stems of Sarcocephalus latifolius (Sm) E.A. Bruce and Massularia acuminata (G. Don) Bullock ex Hoyl. (Rubiaceae)

Ajayi, Emmanuel Olusegun; Odeyemi, Samuel Wale; Otunola, Gloria Aderonke; Afolayan, Anthony Jide

doi:10.14233/ajchem.2017.20444

Cited by 3 publications

(1 citation statement)

References 23 publications

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“…The visual colour change observed on mixing the M. lucida aqueous leaf extract with the AgNO3 solution is similar to reports by Ajayi et al (2017) who synthesized AgNPs with stem extract of Sarcocephalus latifolus also belonging to the family Rubiaceae. This colour change indicating AgNP synthesis was further validated using UVvis spectroscopy and an absorption peak at 460 51 nm was observed.…”

Section: Discussionsupporting

confidence: 87%

Characterization and antimicrobial evaluation of biomimetically synthesized silver nanoparticles using aqueous leaf extract of Morinda lucida Benth. (Rubiaceae)

Egonu,

Chukwuemeka,

Udengwu

2023

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Silver nanoparticles (AgNPs) are silver atom masses that are attracting widespread interest due to their diverse applications, particularly their activity as antimicrobial agents. Physico-chemical methods of AgNP synthesis are associated with high costs, high temperatures and toxic byproducts. Thus, the plant mediated pathway represents a better option. The indigenous medicinal plant Morinda lucida was employed in the fabrication of AgNPs. The nanoparticles were characterized using different analytical techniques and also evaluated for their antimicrobial potential. Phytochemical screening of the plant was also carried out. For the synthesis, 10 ml of aqueous M. lucida leaf extract was added to 90 ml of freshly prepared 3 mM silver nitrate (AgNO3) solution in a flask. The mixture was allowed to stand at ambient temperature, in a dark cupboard for 48 hours. Positive AgNP synthesis, indicated by a colour change from red to brown was further validated by UV-vis spectroscopy wherein an absorption peak at 460.51 nm was recorded. The utilitarian aspects of the particles were further characterized using scanning electron microscopy (SEM), fourier transform infrared (FTIR) spectroscopy, dynamic light scattering (DLS), X-ray diffraction (XRD) and energy dispersive X-ray spectroscopy (EDX). The SEM images showed that particles were round to irregular in shape. Amide, amine, alkene and alkynes were the most occurring functional groups from the FTIR spectra. Quantitative phytochemical analysis revealed the presence of flavonoids, terpenoids, phenols, reducing sugars and alkaloids in varying amounts, which play a significant role in the synthesis and stabilization of the AgNPs. The XRD diffractogram of AgNPs showed two peaks at 45.53° and 77.17° that correspond to miller indices of (200) and (311) respectively and an average crystalline size of 62.60 nm obtained using the Debye-Scherrer’s formula. The DLS result indicated a Z–average size of 235.1 nm and a polydispersity index (PDI) of 0.4. EDX analysis showed that elemental silver (Ag) had the highest atomic concentration of 64.50 %. Using the agar well diffusion assay, the nanoparticles exhibited antimicrobial activity against P. aeruginosa (bacteria) and A. flavus (fungi). It can be concluded that M. lucida is capable of synthesizing stable, small-sized AgNPs with antimicrobial potential.

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Section: Discussionsupporting

confidence: 87%

Characterization and antimicrobial evaluation of biomimetically synthesized silver nanoparticles using aqueous leaf extract of Morinda lucida Benth. (Rubiaceae)

Egonu,

Chukwuemeka,

Udengwu

2023

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Silver nanoparticles biosynthesis by Elaeodendron croceum stem bark and leaves extracts, their anti-bacterial and cytotoxicity activities

Odeyemi

Ajayi

Otunola

et al. 2020

Inorganic and Nano-Metal Chemistry

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In vitro and in vivo toxicity assessment of biologically synthesized silver nanoparticles from Elaeodendron croceum

Odeyemi

Mare

Edkins

et al. 2019

Journal of Complementary and Integrative Medicine

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Background The cytotoxic properties of nanoparticles have attracted a great deal of attention in the field of nanoscience and nanotechnology due to their small size and ability to penetrate cellular membranes. Methods The silver nanoparticles were synthesized using Elaeodendron croceum stem bark and characterized. The oral acute toxicity studies were carried out by administration of 500, 1000, 2000 mg/kg body weight to Wister rats in respective groups. An in vitro cytotoxicity assay was evaluated in MDA-MB-231 breast cancer cells using the WST-1 Cell Proliferation assay. The percentage of cell viability after treatment with aqueous extracts of Elaeodendron croceum (ECE) and Elaeodendron croceum silver nanoparticles (ECAgNPs) was compared with that of paclitaxel. Results The in vivo studies revealed that the LD50 was higher than 2000 mg/kg and there was no significant difference (p>0.05) between the treatment groups compared with the control group for mean organ-to-body weight ratio except in the liver and in all hematological parameters except WBC and hematocrit. Similarly, there was no significant difference (p>0.05) for serum electrolytes (Na+, Mg2+ K+, Cl−, and Ca2+), total protein, urea, ɣ-glutamyl transferase (GGT), Aspartate aminotransferase (AST), Alkaline phosphatase (ALP), Alanine aminotransferase (ALT), albumin, total and conjugated bilirubin between the treatment and the control group. However, there were changes in creatinine, urea, and cholesterol. In the in vitro assays, ECE and ECAgNPs showed IC50 values of 70.87±2.99 and 138.8±3.98 µg/mL respectively against MDA-MB-231 cells compared to paclitaxel, which showed an IC50 value of 80 ng/mL. Conclusion The results showed that the LD50 of the ECE and ECAgNPs in Wister rats was determined to be greater than 2000 mg/kg body weight. The aqueous extract also showed more cytotoxic than the ECAgNPs suggesting that the toxic compounds in aqueous extract were involved in the capping of the AgNPs.

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Characterization and Biological Activities of Silver Nanoparticles Synthesized from Stems of Sarcocephalus latifolius (Sm) E.A. Bruce and Massularia acuminata (G. Don) Bullock ex Hoyl. (Rubiaceae)

Cited by 3 publications

References 23 publications

Characterization and antimicrobial evaluation of biomimetically synthesized silver nanoparticles using aqueous leaf extract of Morinda lucida Benth. (Rubiaceae)

Characterization and antimicrobial evaluation of biomimetically synthesized silver nanoparticles using aqueous leaf extract of Morinda lucida Benth. (Rubiaceae)

Silver nanoparticles biosynthesis by Elaeodendron croceum stem bark and leaves extracts, their anti-bacterial and cytotoxicity activities

In vitro and in vivo toxicity assessment of biologically synthesized silver nanoparticles from Elaeodendron croceum

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