Characterization and Biological Activity of Bacillus thuringiensis Isolates that Are Potentially Useful in Insect Pest Control

Álvarez, Analía; Loto, Flavia del Valle

doi:10.5772/48362

Cited by 2 publications

(2 citation statements)

References 47 publications

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“…For example, we could select pMAN18 as our normalizing vector, divide the activities of all the vectors in each assay by the activity of pMAN18 in the same assay, and thus obtain a new set of results now expressed as a normalized fold change in activity with respect to the pMAN18 value (which now becomes 1.0). This is used for example in (Alvarez and del Valle Loto, 2012, Matsunoshita et al, 2011, Schagat et al, 2007, Ysebrant de Lendonck et al, 2013 or (Zhang et al, 2012, fig. S3).…”

Section: Discussionmentioning

confidence: 99%

Reducing the Standard Deviation in Multiple-Assay Experiments Where the Variation Matters but the Absolute Value Does Not

2013

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When the value of a quantity for a number of systems (cells, molecules, people, chunks of metal, DNA vectors, so on) is measured and the aim is to replicate the whole set again for different trials or assays, despite the efforts for a near-equal design, scientists might often obtain quite different measurements. As a consequence, some systems’ averages present standard deviations that are too large to render statistically significant results. This work presents a novel correction method of a very low mathematical and numerical complexity that can reduce the standard deviation of such results and increase their statistical significance. Two conditions are to be met: the inter-system variations of matter while its absolute value does not, and a similar tendency in the values of must be present in the different assays (or in other words, the results corresponding to different assays must present a high linear correlation). We demonstrate the improvements this method offers with a cell biology experiment, but it can definitely be applied to any problem that conforms to the described structure and requirements and in any quantitative scientific field that deals with data subject to uncertainty.

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Section: Discussionmentioning

confidence: 99%

Reducing the Standard Deviation in Multiple-Assay Experiments Where the Variation Matters but the Absolute Value Does Not

2013

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“…Bt index is defined as the ratio of number of identified Bt colonies to the total number of sporeforming bacilli colonies. Bt was calculated according to Alvarez & del Valle Loto, (2012). The highest Bt index was found to be 0.16 from Tarhara and least index of 0.12 from Vedetar (Fig.…”

Section: Bt Indexmentioning

confidence: 99%

Isolation of Bacillus thuringiensis and its Insecticidal Effect against Galleria mellonella

Limbu

Shrestha

Shakya

et al. 2020

Himalayan J. Sci. Technol.

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Bacillus thuringiensis (Bt) synthesize a large diversity of crystal proteins (Cry and Cyt) during sporulation which exhibit insecticidal activity against insects and protozoa. The main aim of this study was to isolate Bacillus thuringiensis and study its insecticidal effect against Galleria mellonella. Soil samples from four different geographical locations of Koshi Zone viz. Itahari, Tarhara, Dharan and Vedetar of Eastern Nepal were collected. The isolation of Bt was done by acetate selection method. The insect bioassay of Bt isolates were performed against greater wax moth (G. mellonella) by feeding the third instar larvae by extracted crystal spores with three different concentrations. The overall distribution of Bt from the study sample was found to be 30% (30/100). Bt was isolated from all four geographical location with higher incidence; 9 (36%) in Tarhara region followed by Dharan (32%), Itahari (28%) and Vedetar (24%). However, the incidence of Bt with potent insecticidal activity against G. mellonella was reported to be 4% (4/100). The insecticidal activity of isolated Bt between test and control groups was found to be statistically significant (p<0.05). LC50 value of Bt from Tarhara (Tar1) was 388.29μg/mL, Dharan; Drn8 and Drn1 was 416.20μg/mL and 463.15μg/mL respectively and from Vedetar (Vd5) was 476.63μg/mL. In overall study the Bt isolated from Tarhara (Tar1) region exhibited greater incidence, Bt index, efficacy and effective level of LC50 against greater wax moth. Native Bt strains isolated from soil of Eastern Nepal possess effective insecticidal activity and hence can used as biocontrol agent in controlling honeycomb pest like G. mellonella.

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Characterization and Biological Activity of Bacillus thuringiensis Isolates that Are Potentially Useful in Insect Pest Control

Cited by 2 publications

References 47 publications

Reducing the Standard Deviation in Multiple-Assay Experiments Where the Variation Matters but the Absolute Value Does Not

Reducing the Standard Deviation in Multiple-Assay Experiments Where the Variation Matters but the Absolute Value Does Not

Isolation of Bacillus thuringiensis and its Insecticidal Effect against Galleria mellonella

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