2024
DOI: 10.1002/jbio.202300566
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Characterization and classification of pathogenic bacteria using native fluorescence and spectral deconvolution

Anandh Sundaramoorthy,
Ganesan Bharanidharan,
Aruna Prakasarao
et al.

Abstract: Identification and classification of pathogenic bacterial strains is of current interest for the early treatment of diseases. In this work, protein fluorescence from eight different pathogenic bacterial strains were characterized using steady state and time resolved fluorescence spectroscopy. The spectral deconvolution method was also employed to decompose the emission contribution from different intrinsic fluorophores and extracted various key parameters, such as intensity, emission maxima, emission line widt… Show more

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