Characterization and comparison of adipose tissue‐derived cells from human subcutaneous and omental adipose tissues

Toyoda, Mito; Matsubara, Yumiko; Lin, Konghua; Sugimachi, K; Furue, Masutaka

doi:10.1002/cbf.1591

Cited by 69 publications

(83 citation statements)

References 23 publications

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“…At around the same time, Gimble and colleagues identifi ed adipose tissue-derived stromal cells that were capable of both adipogenic and osteogenic induction ( 77, 79, 86 ), a name now applied to more broadly multipotent cells from adipose tissue (87)(88)(89) and sometimes abbreviated as ATSCs (90)(91)(92) or ASCs ( 22 ). Other subtle variations of this name include adipose-derived adult stromal (ADAS) cells ( 47,93,94 ), adipose-derived adult stem (ADAS) cells ( 95,96 ), ASCs ( 97 ), adipose-derived stromal \ stem cells ( 21 ), adipose tissue-derived cells (ATDCs) ( 98,99 ), or simply MSCs ( 87,92 ). Many of these variations are still used today, with combinations often used within a single study ( 87,89,95,97 ).…”

Section: Defining the Ascmentioning

confidence: 99%

Adipose tissue stem cells meet preadipocyte commitment: going back to the future

Cawthorn

Scheller

MacDougald

2012

Journal of Lipid Research

373

323

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with ill health. Such stigmatization distracts from a fascinating aspect of WAT biology: its enormous plasticity as an organ. Indeed, WAT is capable of massive expansion and contraction in response to chronic alterations in energy balance, accounting for as little as 5% body mass in extremely lean athletes ( 1 ) or as much as 60% body mass in morbidly obese individuals ( 2 ). Moreover in rodents, rabbits, and humans, WAT can regenerate following lipectomy ( 3-6 ). This striking degree of plasticity is unique among organs in adults. On a cellular level, WAT expansion is driven by both hypertrophy and hyperplasia of adipocytes ( 7-13 ). Even in nonexpanding WAT, adipocytes renew frequently to compensate for adipocyte death, with approximately 10% of adipocytes renewed annually ( 14,15 ). These data, which indicate that committed adipocyte progenitors (preadipocytes) exist within WAT, are the product of decades of research motivated largely by our desire to understand adipose tissue in the context of obesity and related diseases. Preadipocytes exist in adipose tissueThough the notion of obesity as a disease has existed since at least the time of Hippocrates ( 16 ), investigation into the origins of the adipocyte and the individual contributions of adipocyte hypertrophy and hyperplasia to adipose bulk began in earnest only after the appearance of the stem cell concept, as related to blood cells, in the early 1900s ( 17 ). In the 1940s, histologic observations of the appearance of adipocytes in chambers implanted in the ears of live rabbits suggested that de novo fat formation was Abstract White adipose tissue (WAT) is perhaps the most plastic organ in the body, capable of regeneration following surgical removal and massive expansion or contraction in response to altered energy balance. Research conducted for over 70 years has investigated adipose tissue plasticity on a cellular level, spurred on by the increasing burden that obesity and associated diseases are placing on public health globally. This work has identifi ed committed preadipocytes in the stromal vascular fraction of adipose tissue and led to our current understanding that adipogenesis is important not only for WAT expansion, but also for maintenance of adipocyte numbers under normal metabolic states. At the turn of the millenium, studies investigating preadipocyte differentiation collided with developments in stem cell research, leading to the discovery of multipotent stem cells within WAT. Such adipose tissue-derived stem cells (ASCs) are capable of differentiating into numerous cell types of both mesodermal and nonmesodermal origin, leading to their extensive investigation from a therapeutic and tissue engineering perspective. However, the insights gained through studying ASCs have also contributed to more-recent progress in attempts to better characterize committed preadipocytes in adipose tissue. Thus, ASC research has gone back to its roots, thereby expanding our knowledge of preadipocyte commitment and adipose tissue biology. -Cawthorn, W. P., E. ...

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Section: Defining the Ascmentioning

confidence: 99%

Adipose tissue stem cells meet preadipocyte commitment: going back to the future

Cawthorn

Scheller

MacDougald

2012

Journal of Lipid Research

373

323

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“…Scientists isolated, cultured and characterised these omentum fat derived cells from rat and asserted that these cells exhibited multilineage properties of stem cells which are capable of finding its use in repair and regenerative applications (Tholpady et al 2003;Singh et al 2008). Subsequently, there has been a very scanty citation of similar research with human omentum fat derived stem cells (Baglioni et al 2009;Toyoda et al 2009). Therefore omentum fat raises the issue of rivalry in choosing the best possible source for cell based therapeutics along with bone marrow and subcutaneous fat.…”

Section: Introductionmentioning

confidence: 99%

Surface antigenic profiling of stem cells from human omentum fat in comparison with subcutaneous fat and bone marrow

Dhanasekaran

Somasundaram²,

Kanmani

et al. 2012

Cytotechnology

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Omentum fat derived stem cells have emerged as an alternative and accessible therapeutic tool in recent years in contrast to the existing persuasive sources of stem cells, bone marrow and subcutaneous adipose tissue. However, there has been a scanty citation on human omentum fat derived stem cells. Furthermore, identification of specific cell surface markers among aforesaid sources is still controversial. In lieu of this existing perplexity, the current research work aims at signifying omentum fat as a ground-breaking source of stem cells by surface antigenic profiling of stem cell population. In this study, we examined and compared the profiling of cell surface antigenic expressions of hematopoietic stem cells, mesenchymal stem cells, cell adhesion molecules and other unique markers such as ABCG2, ALDH and CD 117 in whole cell population of human omentum fat, subcutaneous fat and bone marrow. The phenotypic characterization through flowcytometry revealed the positive expressions of CD 34, CD 45, CD 133, HLADR, CD 90, CD 105, CD 73, CD 29, CD 13, CD 44, CD 54, CD 31, ALDH and CD 117 in all sources. The similarities between the phenotypic expressions of omentum fat derived stem cells to that of subcutaneous fat and bone marrow substantiates that identification of ultimate source for curative therapeutics is arduous to assess. Nevertheless, these results support the potential therapeutic application of omentum fat derived stem cells.

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“…Já foi demonstrado que, em coelhos, as CTM-TA da cavidade abdominal apresentam maior potencial de diferenciação osteogê-nica do que as CTM-TA do subcutâneo (42). Mas o contrário se observa em humanos, em que as CTM-TA do subcutâneo é que apresentam maior potencial de diferenciação (43). Além disso, também já foi comprovado que pode haver diferença no potencial osteogênico entre espécies.…”

Section: Discussionunclassified

Efeito in vitro da triiodotironina sob o potencial osteogênico reduzido de células-tronco mesenquimais do tecido adiposo de ratas ovariectomizadas e com osteoporose

Boeloni

Ocarino

Góes

et al. 2013

Arq Bras Endocrinol Metab

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OBJETIVO: Avaliar se a triiodotironina (T3) aumenta a diferenciação osteogênica das células-tronco mesenquimais do tecido adiposo (CTM-TA) de ratas adultas ovariectomizadas e com osteoporose e compará-lo ao de ratas adultas e jovens sem osteoporose. MATERIAIS E MÉTODOS: CTM-TA foram cultivadas em meio osteogênico e distribuídas em sete grupos: 1) CTM-TA de ratas jovens sem osteoporose; 2) CTM-TA de ratas adultas sem osteoporose; 3) CTM-TA de ratas adultas com osteoporose e 4, 5, 6 e 7) CTM-TA de ratas adultas com osteoporose tratadas com T3 (0,01 nM, 1 nM, 100 nM e 1.000 nM). AVALIARAM-SE: atividade da fosfatase alcalina, conversão do dimetiltiazol (MTT), porcentagem de nódulos de mineralização, celularidade e quantificação de transcriptos gênicos para colágeno I, osteocalcina, osteopontina e Bmp-2. RESULTADOS: Independente da dose, T3 reduziu a conversão do MTT, a atividade da fosfatase, a porcentagem de células e a expressão de colágeno I em pelo menos uma das doses e dos períodos estudados (p < 0,05). Mas o tratamento com T3 não alterou o número de nódulos de mineralização e a expressão de osteopontina e Bmp-2 em culturas de CTM-TA de ratas adultas com osteoporose (p > 0,05). CONCLUSÃO: T3 apresenta efeitos negativos sobre alguns fatores envolvidos na diferenciação osteogênica de CTM-TA, sem, no entanto, reduzir a formação de nódulos de mineralização e a expressão de proteínas ósseas.

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Characterization and comparison of adipose tissue‐derived cells from human subcutaneous and omental adipose tissues

Cited by 69 publications

References 23 publications

Adipose tissue stem cells meet preadipocyte commitment: going back to the future

Adipose tissue stem cells meet preadipocyte commitment: going back to the future

Surface antigenic profiling of stem cells from human omentum fat in comparison with subcutaneous fat and bone marrow

Efeito in vitro da triiodotironina sob o potencial osteogênico reduzido de células-tronco mesenquimais do tecido adiposo de ratas ovariectomizadas e com osteoporose

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