Characterization and comparison of Serratia marcescens isolated from edible cactus and from silkworm for virulence potential and chitosan susceptibility

Abstract

Representative strains of Serratia marcescens from an edible cactus plant and silkworms were characterized and a comparison based on their cellular fatty acid composition, 16S rRNA and groE gene sequence analysis as well as silkworm virulence and chitosan susceptibility was carried out. Results from this study indicate that there are no significant differences between the phenotypic and molecular characterization, virulence and chitosan susceptibility of the S. marcescens strains from the cactus plant and silkworms. Silkworms inoculated with S. marcescens from either plant or silkworm resulted in nearly 100% mortality. Chitosan solution exhibited strong antibacterial activity against S. marcescens. This activity increased with the increase of chitosan concentration and incubation time regardless of the strain source. Also, the results indicate that the plant associated S. marcescens maybe plays a possible role in the contamination of humans and animals, in particular silkworms, while chitosan showed a potential to control the contamination caused by S. marcescens.

Enhanced biomass and steviol glycosides in Stevia rebaudiana treated with phosphate-solubilizing bacteria and rock phosphate

“…Cellular fatty acid profiles of B. gladioli 10216 and 10217 showed the presence of 16:0, 16:1 w7c and 18:1 w7c as the major fatty acids which were similar with those described for B. gladioli (Coenye et al 2001). S. marcescens 10238 with 14:00, 16:1 iso I/14:0 3OH, 16:00, 16:1w7c, 17:0 cyclo, and 18:1 w7c as the major fatty acids was related to S. marcescens (Li et al 2011). The presence of major fatty acids 16:00, 16:1 iso I/14:0 3OH, 16:1 w7c and 18:1w7c in E. aerogenes 10208 corresponded to the expected fatty acid composition of E. aerogenes (Wang et al 2010).…”

Synthesis, Characterization, and Antibacterial Activity of Cross-Linked Chitosan-Glutaraldehyde

“…Recently, chitosan, a natural nontoxic biopolymer derived by deacetylation of chitin, a major component of the shells of crustacea such as crab, shrimp, and crawfish, has been applied in the fields of medicine, food, chemical engineering, pharmaceuticals, nutrition, environmental protection and agriculture [12,13]. In particular, chitosan not only has several advantages over other types of bactericides [14], but also has strong antibacterial activity against a variety of bacteria [15,16,17,18,19,20]. However, unfortunately, in our previous studies, chitosan solution possessed a limited antibacterial activity against Bcc bacteria [4,11].…”

Viable bacterial population and persistence of foodborne pathogens on the pear carpoplane

“…This pathogen is known to infect the urinary tract and open wounds in humans . Although this pathogen is not typically associated with foodborne outbreaks, environmental S. marcenscens strains have been reported to be similar to animal and human pathogenic strains as far as virulence, phenotypic and molecular characteristics are concerned . Serratia marcenscens was neither isolated following chlorine drenching, nor after CA storage.…”