Characterization and complete genome sequence of Privateer, a highly prolate <i>Proteus mirabilis</i> podophage

Corban, James Ethan; Ramsey, Jolene

doi:10.7717/peerj.10645

Cited by 6 publications

(5 citation statements)

References 79 publications

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“…A burst was achieved after approximately 15 min, followed by a plateau of 15-20 min. This is rapid compared to lysis profiles of other Novosibovirus members, as listed in Table S2A and B, and reported in literature for Proteus phages [29,46,47]. Approximately 39 plaque forming units (PFUs) of Proteus_virus_309 were recorded per bacterial cell.…”

Section: Physiological Characterizationsupporting

confidence: 57%

Isolation and Characterization of Lytic Proteus Virus 309

Aaron

Zyl

Dicks

2022

Viruses

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Proteus mirabilis is frequently associated with complicated urinary tract infections (UTIs) and is the main cause of catheter-associated urinary tract infections (CAUTIs). Treatment of such infections is complicated and challenging due to the biofilm forming abilities of P. mirabilis. If neglected or mistreated, infections may lead to life-threating conditions such as cystitis, pyelonephritis, kidney failure, and bacteremia that may progress to urosepsis. Treatment with antibiotics, especially in cases of recurring and persistent infections, leads to the development of resistant strains. Recent insights into phage therapy and using phages to coat catheters have been evaluated with many studies showing promising results. Here, we describe a highly lytic bacteriophage, Proteus_virus_309 (41,740 bp), isolated from a wastewater treatment facility in Cape Town, South Africa. According to guidelines of the International Committee on Taxonomy of Viruses (ICTV), bacteriophage 309 is a species within the genus Novosibovirus. Similar to most members of the genus, bacteriophage 309 is strain-specific and lyse P. mirabilis in less than 20 min.

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Section: Physiological Characterizationsupporting

confidence: 57%

Isolation and Characterization of Lytic Proteus Virus 309

Aaron

Zyl

Dicks

2022

Viruses

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“…Other phages related to Lu221 and Hi226 are listed in Table 2 . They include Cronobacter phages vB_CsaP_GAP52, A24, and vB_CsaP_009; Aeromonas phages BUCT695, LAh_6, LAh_8, LAh_9, and 4_4572; Proteus phages 3H10_20, Privateer, and vB_Csap_009; and Kuravirus -like phages, which are abundant E. coli viruses ( 40 , 41 ).…”

Section: Resultsmentioning

confidence: 99%

Isolation and characterization of novel plasmid-dependent phages infecting bacteria carrying diverse conjugative plasmids

Parra,

Cockx,

Lutz

et al. 2024

Microbiol Spectr

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Plasmid-dependent phages infect bacteria carrying conjugative plasmids by recognizing the plasmid-encoded pilus. Despite the high abundance of conjugative plasmids in diverse environments, plasmid-dependent phages have not been widely studied. Since conjugative plasmids often carry antimicrobial resistance genes (ARGs), interfering with conjugation could reduce the spread of ARGs and avoid the appearance of multiresistant superbugs. Our aim was to isolate and characterize plasmid-dependent phages able to infect bacteria carrying diverse conjugative plasmids belonging to the most common plasmid families among Gram-negative pathogens. We isolated two lytic phages from wastewater using an avirulent strain of Salmonella enterica carrying the conjugative IncN plasmid pKM101. Both phages, named Lu221 and Hi226, are novel dsDNA viruses within the class Caudoviricetes with genomes of approximately 76 kb. They showed broad host range infecting Escherichia coli , S. enterica , Kluyvera sp., and Enterobacter sp. carrying conjugative plasmids. They recognize plasmid-encoded receptors from 12 out of 15 tested plasmids, all of them carrying resistance determinants. Phages Lu221 and Hi226 could have the potential to help combat the antimicrobial resistance crisis by reducing ARGs present in conjugative plasmids. IMPORTANCE This work was undertaken because plasmid-dependent phages can reduce the prevalence of conjugative plasmids and can be leveraged to prevent the acquisition and dissemination of ARGs by bacteria. The two novel phages described in this study, Lu221 and Hi226, can infect Escherichia coli , Salmonella enterica , Kluyvera sp. and Enterobacter sp. carrying conjugative plasmids. This was verified with plasmids carrying resistance determinants and belonging to the most common plasmid families among Gram-negative pathogens. Therefore, the newly isolated phages could have the potential to help control the spread of ARGs and thus help combat the antimicrobial resistance crisis.

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“…The one-step growth curve analysis was used to characterize the life cycle of vB_PmiS_PM-CJR and assess its growth kinetics, including the latent period and burst size ( Figure 3 b). The latent period of phage vB_PmiS_PM-CJR was estimated to be approximately 10 min, which can be considered average in comparison to other Proteus phages [ 44 , 45 , 46 ], although direct comparison is difficult due to substantial methodological differences between studies. The growth cycle appears to be complete in approximately 30–35 min as the phage titer begins to plateau after this time period.…”

Section: Resultsmentioning

confidence: 99%

Novel Phage-Derived Depolymerase with Activity against Proteus mirabilis Biofilms

et al. 2021

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The adherence of Proteus mirabilis to the surface of urinary catheters leads to colonization and eventual blockage of the catheter lumen by unique crystalline biofilms produced by these opportunistic pathogens, making P. mirabilis one of the leading causes of catheter-associated urinary tract infections. The Proteus biofilms reduce efficiency of antibiotic-based treatment, which in turn increases the risk of antibiotic resistance development. Bacteriophages and their enzymes have recently become investigated as alternative treatment options. In this study, a novel Proteus bacteriophage (vB_PmiS_PM-CJR) was isolated from an environmental sample and fully characterized. The phage displayed depolymerase activity and the subsequent genome analysis revealed the presence of a pectate lyase domain in its tail spike protein. The protein was heterologously expressed and purified; the ability of the purified tail spike to degrade Proteus biofilms was tested. We showed that the application of the tail spike protein was able to reduce the adherence of bacterial biofilm to plastic pegs in a MBEC (minimum biofilm eradication concentration) assay and improve the survival of Galleria mellonella larvae infected with Proteus mirabilis. Our study is the first to successfully isolate and characterize a biofilm depolymerase from a Proteus phage, demonstrating the potential of this group of enzymes in treatment of Proteus infections.

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Characterization and complete genome sequence of Privateer, a highly prolate Proteus mirabilis podophage

Cited by 6 publications

References 79 publications

Isolation and Characterization of Lytic Proteus Virus 309

Isolation and Characterization of Lytic Proteus Virus 309

Isolation and characterization of novel plasmid-dependent phages infecting bacteria carrying diverse conjugative plasmids

Novel Phage-Derived Depolymerase with Activity against Proteus mirabilis Biofilms

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