Characterization and culture of sinusoidal endothelium from normal rat liver: Lipoprotein uptake and collagen phenotype

Irving, Michael G.; Roll, F J; Huang, S.-L.; Bissell, D. Montgomery

doi:10.1016/0016-5085(84)90188-4

Cited by 168 publications

(83 citation statements)

References 30 publications

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“…Since we did not observe colocalization of CD31 staining with significant autoradiographic signals in any of our tissue sections, endothelial cells are unlikely to contribute to hepatic interstitial collagen synthesis in relevant quantity. Our data are therefore well in keeping with those studies on purified cultured endothelial cells, which indicated production of basement membrane components but not of interstitial collagens by these cells (Irving et al 1984). Although repeatedly suggested as the major source of liver collagens, hepatocytes did not display significant procollagen transcript levels in any of our samples in comparison with control hybridizations using sense RNA probes.…”

Section: Classessupporting

confidence: 90%

Heterogeneity of liver cells expressing procollagen types I and IV in vivo

Herbst

Frey²,

Heinrichs

et al. 1997

Histochemistry and Cell Biology

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The extracellular matrix of fibrotic liver is predominantly produced by mesenchymal cells, the in vivo phenotype of which is poorly defined. We report on the application of combined immunohistology and in situ hybridization with [35S]-labeled alpha 1(I) and alpha 1(IV) procollagen RNA probes. In CCl4-treated rats, all alpha 1(I) procollagen-producing cells were vimentin positive but cytokeratin negative; over 90% expressed desmin, a marker of rat liver stellate cells. alpha 1(I) Procollagen-expressing, desmin-negative cells were confined to portal tract and perivascular stroma. Similarly, alpha 1(I) procollagen gene transcripts were, in all instances, colocalized with vimentin in human liver. In fibrotic specimens, over 70% of these cells expressed alpha-actin. Antibodies against epithelial, endothelial, and Kupffer cells, granulocytes, and lymphocytes did not react with alpha 1(I) procollagen RNA-expressing cells. Localization, morphology, and immunophenotype of alpha 1(I) procollagen-expressing cells indicated stellate cells and portal/vascular fibroblasts, but not epithelial cells, to be sources of hepatic interstitial collagen. However, most alpha 1(IV)-expressing human liver cells were identified as endothelial cells, the remaining cells were (myo-)fibroblastic and bile duct epithelial cells, but not hepatocytes. This indicates synthesis of procollagen type IV from both sides of the basement membrane and suggests an active participation of endothelial cells in the process of sinusoidal capillarization.

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Section: Classessupporting

confidence: 90%

Heterogeneity of liver cells expressing procollagen types I and IV in vivo

Herbst

Frey²,

Heinrichs

et al. 1997

Histochemistry and Cell Biology

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“…Furthermore, antibody reactivity decreased with passage number, which was similar to the findings of Maciag et al [180] and Hormonia [201]. The finding that liver-derived HSE cells were negative for factor VIII; vWF antigen was consistent with our inability to stain mouse liver sinusoids with this antibody and with previous reports on the distribution of this antigen in vivo [90]. Liver sinusoidal endothelial cells selectively Table 3.…”

Section: Heterogeneity Of Cultured Endothelial Cellssupporting

confidence: 81%

“…Another antigen commonly used to identify the endothelial nature of cells, factor VIII-related antigen (FVIII-RAg), is present in most organ endothelia except liver sinusoids [90], renal gtomerular capillaries [91], or in lymphatic endothelium [92]. In addition, there appears to be species-specificity, since many of the commercially available antibodies against human FVIlI-RAg do not stain mouse or rat endothelia in tissue section (Belloni, unpublished observation).…”

Section: Antigenic Heterogeneity Of Endotheliummentioning

confidence: 99%

“…Analysis of collagens synthesized by endothelial cells from six different mammalian species and from different types of bovine vessels suggests that large vessel bovine endothelial cells primarily produce interstitial collagen type III and basement membrane types IV and V, while bovine capillary endothelial cells synthesize types I, 11I, and V [154]. Type IV collagen is the only collagen associated with liver sinusoidal endothelium [90]. The major collagens produced by endothelial cells from other species, including human, appear to be types IV and V [155].…”

Section: Matrix-associated Moleculesmentioning

confidence: 99%

“…During the last decade techniques have been reported for the isolation and culture of microvascular endothelial cells from diverse tissues including rat cerebral tissue [182,183], liver sinusoids [90], and lymph nodes [184]; mouse cerebrum [185][186][187] and liver sinusoids [6,187]; rabbit pulmonary tissue [188] and marginal vessels [189]; bovine adrenal glands [190] and retina [191]; and human skin [192,193], adipose tissue [194], glomeruli [195], and brain [196]. Although the reported techniques for isolating microvascular endothelial cells are similar, the reported conditions of cell culture are diverse, and therefore interpreting the effects of various agents such as growth factors in microvascular cell culture remains unclear [197][198][199][200].…”

Section: Heterogeneity Of Cultured Endothelial Cellsmentioning

confidence: 99%

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Microvascular endothelial cell heterogeneity: Interactions with leukocytes and tumor cells

Belloni

Tressler

1990

Cancer Metast Rev

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Endothelium constitutes a highly specialized organ that lines the vascular system and lymphatic channels. This organ is a complex network of arteries, veins, and microvessels that differ in size, structure, and function. The unique and strategic location imposes functional demands on the endothelium that are far greater than just being a passive barrier. Endothelial cells have the ability to differentiate both in structure and function in response to the needs of diverse tissue environments, making this organ extremely heterogeneous. Although vascular endothelial cells share certain common properties, they differ in regard to structure, antigenic and cell surface determinants, adhesion molecules, and metabolic function. The unique cell surface profiles expressed by endothelial cells in different tissue locations can be recognized by specific populations of circulating leukocytes or tumor cells, which contribute to their arrest and invasion patterns. This article attempts to review our current understanding of endothelial cell heterogeneity and its significance to patterns of leukocyte and tumor cell trafficking.

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