Characterization and Cytotoxic Evaluation of Silver and Gold Nanoparticles Produced with Aqueous Extract of Lavandula dentata L. in Relation to K-562 Cell Line

Abstract

Metallic nanoparticles have great potential as a chemotherapeutic agent. The aim of this study was to develop and characterize silver and gold nanoparticles using a simple method, as well as evaluating the potential cytotoxic activity in relation to the K-562 cell line. For the synthesis, a solution containing the metallic ions was subjected to magnetic stirring with the aqueous extract of Lavandula dentata L. and a change of colour was observed. With the data obtained from the analyses we concluded that the nanoparticles were successfully obtained by a simple and green method using the aqueous extract of L. dentata. The obtained nanoparticles presented a reduced size, a low level of polydispersion, and a homogenous spherical shape. The nanoparticles presented intense and characteristic diffraction peaks, which could be correlated to the planes of the centred cubic structure of the silver and gold. The two formulations presented predominantly crystalline characteristics. The infrared analysis suggested that the amides and alcohols present in the samples may have been responsible for the reduction and limitation of the size and dispersion of the silver and gold nanoparticles. The cytotoxic assay showed that the nanoparticles demonstrated great potential to reduce the cell viability of the K-562 cell line, especially the gold nanoparticles.

Antimicrobial Orthodontic Wires Coated with Silver Nanoparticles

“…Is an expected result, sice hydrothermal synthesis, the method employed for AgNPs production in this work, allows controlling particles morphology and size during the synthesis [14]. Justus and coauthors [24] obtained silver nanoparticles from natural sources and observed its size around 30nm, results close to those found in this work.…”

“…SEM is an important technique used for the study of nanoparticle morphology and size, as well as allowing for the analysis of sample homogeneity [24] and, according to its images, most particles covering orthodontic wires presented spherical-like shape and nanometric size, inferior to 20nm. Is an expected result, sice hydrothermal synthesis, the method employed for AgNPs production in this work, allows controlling particles morphology and size during the synthesis [14].…”

Antineoplastic activity of biogenic silver and gold nanoparticles to combat leukemia: Beginning a new era in cancer theragnostic

“…Justus et al compared the anti-leukemic activity of the AuNPs and AgNPs, which were fabricated using the aqueous extract of Lavandula dentata L, against K-562 cells using MTT assay. After 72 h of treatment, 250 µL of AuNPs stock (0.1 mM) induced 76.2% cell inhibition, whereas AgNPs induced 36.1% reduction in cell viability [115] .…”

The nanocomposites designs of phytomolecules from medicinal and aromatic plants: promising anticancer-antiviral applications

“…However, these results from the research presented may be useful to support the use of lavender-distilled essential oils as a promising anticancer agent in cancer treatment, as well as in vivo studies to identify mechanisms underlying anti-cancer effects [ 30 ]. Another study has shown that nanoparticles (LdAuNPs) produced with lavender have great potential to reduce K-562 cell viability, a myelogenous leukemia cell line [ 45 ].…”