Characterization and dietary regulation of oligopeptide transporter (PepT1) in different ploidy fishes

Liu, Zhen; Zhou, Yi; Liu, Shaojun; Zhao, Qiong; Feng, Junchang; Lu, Shuangqing; Xiong, Gang; Xie, Dizhi; Zhang, Jianshe; Liu, Yun

doi:10.1016/j.peptides.2013.12.017

Cited by 31 publications

(18 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…; Liu et al . , ). Fish protein hydrolysate is obtained by enzymic or formic acid hydrolysis and includes rich small peptides and amino acids (Liaset et al .…”

Section: Introductionmentioning

confidence: 99%

“…Peptide transporter 1 (PepT1) is primarily localized at the epithelial cells of brush-border membranes in the intestine and plays a crucial role in protein nutrition by mediating the uptake of dietary amino acids in di-and tripeptide form (Daniel 2004). Several studies suggested that regulated PepT1 expression by dietary-free amino acid, peptides and intact protein in fish could affect growth performance directly and/or indirectly (Ostaszewska et al 2009(Ostaszewska et al , 2010aBakke et al 2010;Liu et al 2013Liu et al , 2014. Fish protein hydrolysate is obtained by enzymic or formic acid hydrolysis and includes rich small peptides and amino acids (Liaset et al 2000(Liaset et al , 2002Mach & Nortvedt 2011).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The effect of ultrafiltered fish protein hydrolysate level on growth performance, protein digestibility and mRNA expression of PepT1 in juvenile turbot (Scophthalmus maximusL.)

Wei

Liang

et al. 2015

Aquacult Nutr

View full text Add to dashboard Cite

The intention of the study was to investigate the effect of ultrafiltered fish protein hydrolysate (UF) level on growth, feed utilization, apparent digestibility coefficients and proximal intestine peptide transporter 1 (PepT1) mRNA level for juvenile turbot (Scophthalmus maximus L.). Experimental diets (UF‐0, UF‐5, UF‐10, UF‐15 and UF‐20) were prepared containing about 68% plant protein, and fish meal protein was, respectively, replaced by 0%, 5%, 10%, 15% and 20% UF of dietary protein. Diet PP contained about 78% plant protein, and diet CAA contained about 10% crystalline amino acid mixture. All diets were fed to seven triplicate groups of turbot (initial weight 16.05 ± 0.03 g) for 68 days. Fish fed diet UF‐10 had an increasing tendency in growth compared with diets contained UF, while dietary UF level was not significantly correlated with specific growth rate and feed intake. Feed efficiency, protein efficiency ratio and protein productive value significantly correlated with dietary UF level, and fish fed diets contained low‐level UF had higher digestibility than that diets UF‐0, PP and CAA. There was a decreasing tendency in PepT1 expression level with dietary UF level. The results indicated that low‐level UF showed a positive effect on growth and feed utilization in juvenile turbot.

show abstract

“…; Liu et al . , ). Fish protein hydrolysate is obtained by enzymic or formic acid hydrolysis and includes rich small peptides and amino acids (Liaset et al .…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The effect of ultrafiltered fish protein hydrolysate level on growth performance, protein digestibility and mRNA expression of PepT1 in juvenile turbot (Scophthalmus maximusL.)

Wei

Liang

et al. 2015

Aquacult Nutr

View full text Add to dashboard Cite

show abstract

“…Our previous results demonstrated that dietary sodium butyrate increased mRNA and protein levels of PepT1 in the intestines of red crucian carp. Additionally, we demonstrated that the transcription factor CDX2 may play a regulatory role in butyrate‐induced PepT1 expression in fishes of different ploidy (Liu et al., ). In this study, we extended our study to determine the effects of sodium butyrate on expression of Ci PepT1/ Ci Sp1/ Ci CDX2 in the grass carp intestine.…”

Section: Discussionmentioning

confidence: 87%

“…Like other breeding fish, the diet of grass carp requires certain dietary protein levels and protein sources. Our previous studies have shown that dietary protein and feed additives have a significant effect on intestinal PepT1, a key mediator of protein absorption (Liu et al., , ). However, the transcriptional regulatory mechanism of PepT1 in fish remains unknown.…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization and nutritional regulation of specificity protein 1 (Sp1) in grass carp (Ctenopharyngodon idella )

Tang

et al. 2017

Aquacult Nutr

View full text Add to dashboard Cite

Specificity protein 1 (Sp1) is a ubiquitous nuclear factor in eukaryotic cells and plays a crucial role in the regulation of di-/tripeptide absorption in the intestine. However, the function of Sp1 in bony fish remains poorly understood. In this study, a fish Sp1 gene (designated CiSp1) was identified from Ctenopharyngodon idella. The deduced CiSp1 protein contains structural features typical of Sp1 family proteins, including a glutamine-rich (Q-rich) region and three typical Cys2His2 zinc fingers. Quantitative real-time PCR analysis revealed ubiquitous expression of CiSp1 in all tested tissues and embryonic developmental stages of grass carp. In addition, CiSp1 expression in the intestine was significantly increased after feeding with fish meal and 270 g/kg dietary protein. Moreover, in vitro and in vivo glutamine dipeptide treatments showed similar stimulatory effects on the expression of the transcription factors CiSp1 and CiCDX2 and their potential target gene CiPepT1 in the intestines of grass carp. Finally, our results demonstrated that the mRNA expression of CiSp1/CiCDX2/CiPepT1 in the intestine was significantly increased after treatment with sodium butyrate in vitro and in vivo. Altogether, these results demonstrate for the first time that grass carp possesses a functional Sp1 and that its expression is regulated by dietary protein and feed additives. K E Y W O R D S characterization, Ctenopharyngodon idella, expression profile, nutritional regulation, specificity protein 1

show abstract

“…Liu et al. () found that both high protein level diets and low protein level diets promoted PepT1 expression in the intestine of triploid red crucian carp ( Carassius aumtus ). The study in rainbow trout also revealed a significant increase in PepT1 mRNA expression of fish fed experimental diets supplemented with dipeptides and free amino acids (Ostaszewska et al., ).…”

Section: Discussionmentioning

confidence: 99%

Dietary krill hydrolysates affect the expression of growth-related genes in juvenile turbot (Scophthalmus maximus L.)

et al. 2018

View full text Add to dashboard Cite

A 10‐week feeding experiment in indoor flow‐through seawater system was conducted to investigate the effects of dietary krill hydrolysate on the expression of growth‐related genes in juvenile turbot (Scophthalmus maximus L.; initial body weight 9.45 ± 0.01 g). Three isonitrogenous and isolipidic experimental diets containing high plant protein were formulated to contain 0 (control), 50 g/kg (LKH) and 100 g/kg (HKH) krill protein hydrolysate (KH) to replace fishmeal, respectively. Triplicate groups of 30 fish were fed for 10 weeks to apparent satiation twice daily. At the end of the feeding trial, the mRNA expressions of insulin‐like growth factor (IGF‐1) gene in liver, peptide transporters (PepT1) gene in pyloric caeca and proximal intestine and neuropeptide Y (NPY) gene in brain in all groups were determined. IGF‐1, PepT1 and NPY expression levels in HKH group were significantly increased compared with those of LKH and control (p < 0.05), which was consistent with the SGR, feed efficiency, PER and PPV. These results indicated that dietary 100 g/kg krill hydrolysate could improve growth performance and upregulate the mRNA expression of IGF‐1, PepT1 and NPY genes in juvenile turbot.

show abstract

Characterization and dietary regulation of oligopeptide transporter (PepT1) in different ploidy fishes

Cited by 31 publications

References 36 publications

The effect of ultrafiltered fish protein hydrolysate level on growth performance, protein digestibility and mRNA expression of PepT1 in juvenile turbot (Scophthalmus maximusL.)

The effect of ultrafiltered fish protein hydrolysate level on growth performance, protein digestibility and mRNA expression of PepT1 in juvenile turbot (Scophthalmus maximusL.)

Molecular characterization and nutritional regulation of specificity protein 1 (Sp1) in grass carp (Ctenopharyngodon idella )

Dietary krill hydrolysates affect the expression of growth-related genes in juvenile turbot (Scophthalmus maximus L.)

Contact Info

Product

Resources

About