Characterization and diversity of Indian isolates of Ralstonia solanacearum inciting bacterial wilt of tomato

Singh, D. B.; Chaudhary, Garima; Yadav, D.

doi:10.1007/s42360-021-00365-9

Cited by 12 publications

(20 citation statements)

References 20 publications

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“…Bacterial wilt is a major constraint for production of eggplant ( Solanum melongena also known as brinjal or aubergine), tomato ( S. lycopersicum ), and pepper ( Capsicum spp.) in South Asia (Sinha, SK 1986; Sood and Singh 1993; Adhikari et al 1993; Pradhanang et al 2000; Pradhanang and Momol 2001; Ahmed et al 2013; Adhikari et al 1997; Singh et al 2010). Host resistance is the most practical and sustainable approach for management of this disease, however very few bacterial wilt-resistant cultivars are available (López and Biosca 2005) (Pandiyaraj et al 2019).…”

Section: Introductionmentioning

confidence: 99%

Characterization ofRalstonia pseudosolanacearumdiversity and screening host resistance to manage bacterial wilt in South Asia

Subedi,

Cowell,

Cope-Arguello

et al. 2023

Preprint

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In South Asia, bacterial wilt pathogens in the Ralstonia solanacearum species complex (RSSC) impose major constraints on eggplant, tomato, and pepper production. To improve the efficacy of bacterial wilt management, the goals of this study were to (1) conduct a survey of RSSC pathogens in Bangladesh and Nepal, (2) characterize the genetic diversity of these isolates, and (3) screen 37 tomato, eggplant, and pepper accessions for resistance to six representative isolates from South Asia. We isolated 99 isolates from Bangladesh and 20 isolates from Nepal and determined that all are phylotype I isolates of the Ralstonia pseudosolanacearum species. We sequenced and assembled draft genomes for 25 isolates. Phylogenomic analyses suggest that there is a wide diversity of endemic phylotype I isolates in South Asia, and possible introductions of two clonal phylotype I lineages into Bangladesh and Nepal. We contextualize our newly described isolates based on prior reports of RSSC diversity in South Asia and global reports of RSSC pathogens on eggplant and pepper. Greenhouse trials revealed multiple tomato, eggplant, and pepper accessions that exhibit promising levels of resistance to six phylotype I isolates from South Asia.

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Section: Introductionmentioning

confidence: 99%

Characterization ofRalstonia pseudosolanacearumdiversity and screening host resistance to manage bacterial wilt in South Asia

Subedi,

Cowell,

Cope-Arguello

et al. 2023

Preprint

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“…Bacterial wilt caused by Ralstonia pseudosolanacearum (Formely Ralstonia solanacearum (Smith) Yabuuchi (1995) [1] is a serious disease of tomato (Solanum lycopersicum L.) in tropical, subtropical and temperature areas of the world. In India, the bacterial wilt disease has been reported mostly in coastal, hilly, as well as foothills areas, in India including in the state of Goa, Karnataka, Kerala, Maharashtra, Odisha, Jharkhand, and West Bengal, Himachal Pradesh, Jammu & Kashmir, Uttarakhand and north-eastern states [2,3]. The disease causes very heavy losses, varying from 2 to 90 % in different agro-climatic conditions in India [3].…”

Section: Introductionmentioning

confidence: 99%

“…In India, the bacterial wilt disease has been reported mostly in coastal, hilly, as well as foothills areas, in India including in the state of Goa, Karnataka, Kerala, Maharashtra, Odisha, Jharkhand, and West Bengal, Himachal Pradesh, Jammu & Kashmir, Uttarakhand and north-eastern states [2,3]. The disease causes very heavy losses, varying from 2 to 90 % in different agro-climatic conditions in India [3]. Application of microbial antagonists is being emerged method to were studied against bacterial wilt pathogen R. pseudosolanacearum using dual culture technique as described by Singh et al [6] under in vitro conditions.…”

Section: Introductionmentioning

confidence: 99%

Suppression of Tomato Bacterial Wilt Incited by Ralstonia pseudosolanacearum Using Polyketide Antibiotic-Producing Bacillus spp. Isolated from Rhizospheric Soil

2022

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Bacillus spp. has the potential to control bacterial and fungal diseases of crops. In vitro study, Bacillus amyloliquefaciens DSBA-11 showed best to inhibit the growth of Ralstonia pseudosolanacearum as compared to Bacillus cereus JHTBS-7, B. pumilus MTCC-7092, B. subtilis DTBS-5 and B. licheniformis DTBL-6.Three primers sets from nucleotide sequences of polyketide antibiotic synthase genes viz., macrolactin, difficidin and bacillaene of B. amyloliquefaciens FZB42 were designed and standardized protocol for simultaneous detection of polyketide antibiotics-producing strains of Bacillus spp. by multiplex—PCR with products size of 792 bp, 705 bp and 616 bp respectively. All the strains of B. amyloliquefaciens contained three polyketide antibiotic synthase genes, and B. subtilis possessed difficidin and macrolactin, whereas B. cereus JHTBS-7, B. pumilus MTCC-7092 and B. licheniformis DTBL-6 did not contain any polyketide antibiotic genes. By using this technique, polyketide-producing strains of Bacillus spp. were screened within a short period with high accuracy. These polyketide synthase genes were cloned by using a T&A vector to study the role of these genes in producing antibiotics that suppressed the growth of R. pseudosolanacearum under both in vitro and in vivo conditions. Bio-efficacy of cloned products of these genes macrolactin, bacillaene, and difficidin along with parent strain B. amyloliquefaciens DSBA-11 inhibited the growth of R. pseudosolanacearum and formed 1.9 cm2, 1.9 cm2, 1.7 cm2 and 3.3 cm2 inhibition area under in vitro conditions respectively. Minimum bacterial wilt disease intensity (29.3%) with the highest biocontrol efficacy (57.72%) was found in tomato cv. Pusa Ruby (susceptible to wilt disease) was treated with B. amyloliquefaciens DSBA-11 followed by cloned products of difficidin and macrolactin under glasshouse conditions. Hence, the developed multiplex protocol might be helpful for screening polyketide antibiotics producing potential strains of Bacillus spp. from soil which can apply for managing the wilt disease of tomatoes. The polyketide antibiotics produced by bacteria might have a significant role suppression of R. pseudosolanacearum due to the disintegration of cells.

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“…Além disto, este gênero é encontrado em uma grande variedade de plantas e sobrevivem muito bem as condições de estresse. Este gênero já foi reportado promovendo o crescimento de culturas de importância econômica como milho EGAMBERDIYEVA, 2007;GOND et al, 2015), arroz (ASHRAFUZZAMAN et al, 2009), soja (RAMESH et al, 2014, tomate (MENA-VIOLANTE; OLALDE-PORTUGAL, 2007) e trigo (SINGH et al, 2012).…”

Section: Importância Dos Microrganismos Para As Plantasunclassified

“…Ressalta-se também que as variedades estavam em condições idênticas para seu crescimento e desenvolvimento, no entanto, a RB867515 apresentou uma taxa menor para SOD.Em geral, as RPCPs influenciam propriedades fisiológicas como a fotossíntese das plantas hospedeiras. Estudos anteriores demonstraram que o AIA é capaz de aumentar o teor de pigmentos, a taxa fotossintética, a condutância estomática e o acúmulo de açúcares, como glicose, frutose e açúcares solúveis totais nas plantas(HAYAT et al, 2009;LI et al, 2019;SINGH; PRASAD, 2015). Além disso, esta auxina, eleva a taxa fotossintética aumentando a densidade das nervuras foliares coordenada por meio de sua colocação bem organizada, o que impulsiona a melhoria na capacidade fotossintética das folhas(MCADAM et al, 2017).…”

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Aspectos biológicos da promoção de crescimento vegetal por >i<Bacillus thuringiensis>/i< RZ2MS9 com enfoque na adubação fosfatada

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À minha orientadora Dra. Maria Carolina Quecine, pela paciência, por acreditar em mim e pela motivação para o desenvolvimento desta pesquisa e pela ciência.À minha mãe Adriana e seu companheiro Jovair, pelo apoio incondicional e pelo incentivo ao longo de todo minha formação acadêmica.Ao meu irmão Rafael e sua companheira Aline, pela motivação para busca incessante de conhecimento.À minha madrinha Rejane, pelo apoio e incentivo.À Ana Carolina Machado, por ser espelho de mim mesma e me mostrar do que sou capaz, quando estava tudo meio nublado.Aos meus amigos Malu Sena e Renato Cardoso, por serem pessoas inspiradoras, pela amizade e suporte emocional.À Olga, pela alegria que me proporciona.Às companheiras de apartamento, pelas conversas, jantas compartilhadas, apoio e por serem minha família em Piracicaba.Aos meus colegas pesquisadores, pela troca de ideias científicas desde 2014.Aos colegas do Laboratório de Genética de Microrganismos, pela ajuda nos experimentos em casa de vegetação, pela ajuda em extrair um RNA de qualidade e pelas trocas de ideias, artigos e conhecimento.Ao técnico do Laboratório de Genética de Microrganismos Zezo e demais funcionários, pelo convívio e suporte essencial para o desenvolvimento desta pesquisa.Aos professores do Programa de Pós-graduação em Genética e Melhoramento de Plantas, pela contribuição em minha formação.

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Characterization and diversity of Indian isolates of Ralstonia solanacearum inciting bacterial wilt of tomato

Cited by 12 publications

References 20 publications

Characterization ofRalstonia pseudosolanacearumdiversity and screening host resistance to manage bacterial wilt in South Asia

Characterization ofRalstonia pseudosolanacearumdiversity and screening host resistance to manage bacterial wilt in South Asia

Suppression of Tomato Bacterial Wilt Incited by Ralstonia pseudosolanacearum Using Polyketide Antibiotic-Producing Bacillus spp. Isolated from Rhizospheric Soil

Aspectos biológicos da promoção de crescimento vegetal por >i<Bacillus thuringiensis>/i< RZ2MS9 com enfoque na adubação fosfatada

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