Characterization and enhanced production of enterocin HJ35 byEnterococcus faecium HJ35 isolated from human skin

Yoon, Yoh Chang; Park, Hye Jung; Lee, Na‐Kyoung; Paik, Hyun‐Dong

doi:10.1007/bf02931845

Cited by 6 publications

(3 citation statements)

References 30 publications

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“…All experimentswere conducted in a shaker incubator at150rpmincubation temperature of 37ᴼ C for18h under anaerobic condition. This result is in partial agreement with the findings of [29] in the batch fermentation of Ent. faecium HJ35, enterocin HJ35, that the production of bacteriocin was produced at the mid-log growth phase, reaching a maximum production of up to 2,300 AU/mL during the late stationary phase.…”

Section: Growth Curve Of the Isolatesupporting

confidence: 92%

Optimization of temperature and pH forthe growth and bacteriocin production of Enterococcus faecium. B3L3

Yusuf¹

2012

iosrphr

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:-The knowledge of the optimum condition for growth of Ent. FaeciumstrainB3L31 and its bacteriocin production are important when deciding on mass production of both the organism and its bacteriocin for probiotic use and as an antimicrobial agent respectively.The optimum condition viz: temperature, pH and incubation period were used to determined parameters most suitable for growthandbacteriocin production fromEnt. faecium B3L3. These three variables of optimization for growth and bacteriocin production were determined based on the growth curves. The bacteriocin production was done by growing strain of LAB onMRS broth the bacteriocin harvest was done following the chloroform precipitation. Resultobtained showed that maximum bacteriocin production occur at the tail end of the exponential phase. The most optimum pH and temperaturefor growth and bacteriocin production were found to be pH8 and37 O Crespectively. The partially purified protein when resolved on SDS-PAGE was shown to be of molecular weight 10kD. The bacteriocin also produced an inhibitory action against methicillin resistance Staphylococcus aureus.

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Section: Growth Curve Of the Isolatesupporting

confidence: 92%

Optimization of temperature and pH forthe growth and bacteriocin production of Enterococcus faecium. B3L3

Yusuf¹

2012

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“…This is in partial agreement with the results obtained by Leroy and De Vuyst (2002), in which production of enterocin RZC5 (from E. faecium) occurred in the early growth phase. The production of enterocin HJ35 by E. faecium HJ35 also occurred in the mid-log growth phase, reaching maximum production during the late stationary phase (Yoon et al, 2005). Moshood and Tengku Haziyamin (2012) showed that maximum bacteriocin production of E. faecium B3L3 occurs at the end of the exponential phase.…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial compounds produced by Enterococcus spp. isolates from fecal samples of wild South American fur seals

Comerlato¹,

Buboltz²,

Santestevan³

et al. 2016

J. Microbiol. Antimicrob.

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The aims of this study were to identify bacteriocinogenic activity in 13 enterococci isolated from fecal samples of wild South American (Arctocephalus australis) and Subantarctic fur seals (Arctocephalus tropicalis); to determine the physicochemical characteristics and antimicrobial spectrum of antimicrobial compounds against Gram-positive and Gram-negative bacteria; and to evaluate the presence of bacteriocin structural genes by PCR. Out of 13 enterococci screened for antimicrobial activity, five enterococci showed activity against Listeria monocytogenes ATCC 35152, an important pathogen linked to food. Of these, only the E. mundtii strain J5 maintained the activity after the pH was adjusted (pH 6.5). The activity of antimicrobial compounds from the E. mundtii strain J5 (ACs-J5) was lost after proteolytic enzyme treatment; however, the activity was maintained after heat, pH (acidic and basic conditions) and chemical treatment. ACs-J5 showed narrow spectrum activity. Only the mundticin KS gene was detected in the J5 strain and no plasmid was present. In conclusion, the properties presented by ACs-J5 make it a valuable biopreservative in food industries in avoiding pathogenic microorganisms such as L. monocytogenes and it should be a good candidate for probiotic application.

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“…This reactor facilitates efficient operation with high cell viability by maintaining the cells in continuous growth mode as if in a permanent exponential growth phase. On the other hand, it has been reported that most bacteriocins show a rather sharp decrease in activity at the end of the exponential growth phase [1,2,7,20] Thus, in order to enhance the productivity of nisin Z the cells must be kept in continuous growth or in a permanent exponential growth phase as in the synchronized fresh cell bioreactor.…”

Section: Introductionmentioning

confidence: 99%

Production of nisin Z using Lactococcus lactis IO-1 from hydrolyzed sago starch

Carvajal-Zarrabal

Nolasco-Hipólito²,

Bujang

et al. 2009

J Ind Microbiol Biotechnol

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A membrane bioreactor for production of nisin Z was constructed using Lactococcus lactis IO-1 in continuous culture using hydrolyzed sago starch as carbon source. A strategy used to enhance the productivity of nisin Z was to maintain the cells in a continuous growth at high cell concentration. This resulted in a volumetric productivity of nisin Z, as 50,000 IU l(-1) h(-1) using a cell concentration of 15 g l(-1), 30( degrees )C, pH 5.5 and a dilution rate of 1.24 h(-1). Adding 10 g l(-1) YE and 2 g l(-1) polypeptone, other inducers were unnecessary to maintain production of nisin. The operating conditions of the reactor removed nisin and lactate, thus minimizing their effects which allowed the maintenance of cells in continuous exponential growth phase mode with high metabolic activity.

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Characterization and enhanced production of enterocin HJ35 byEnterococcus faecium HJ35 isolated from human skin

Cited by 6 publications

References 30 publications

Optimization of temperature and pH forthe growth and bacteriocin production of Enterococcus faecium. B3L3

Optimization of temperature and pH forthe growth and bacteriocin production of Enterococcus faecium. B3L3

Antimicrobial compounds produced by Enterococcus spp. isolates from fecal samples of wild South American fur seals

Production of nisin Z using Lactococcus lactis IO-1 from hydrolyzed sago starch

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