Characterization and enzymatic properties of protein kinase ACR4 from Arabidopsis thaliana

Zhao, Yu; Liu, Xuehe; Xu, Ziyan; Yang, Hui; Li, Jixi

doi:10.1016/j.bbrc.2017.05.163

Cited by 4 publications

(4 citation statements)

References 20 publications

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“…Within the Mid group, high‐quality annotated candidate loci were located in ACR4 , CaMRLK , iPGAM, and RH38 , and all the genes are associated with high‐altitude adaptation and thermal stress. iPGAM is primarily involved in vegetative growth and stomatal movement, and ACR4 plays important roles in cell division and differentiation, both of which are involving in plant growth (Zhao et al, 2017). RH38 is a member of the DEAD‐box family primarily involved in abiotic stress, and a study found RH38 having upregulated expression under cold stress but more sensitive to thermal stress in Arabidopsis thaliana (Chen & Peng, 2016).…”

Section: Discussionmentioning

confidence: 99%

Staying in situ or shifting range under ongoing climate change: A case of an endemic herb in theHimalaya‐HengduanMountains across elevational gradients

Lin

Liu

Landis

et al. 2023

Diversity and Distributions

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Aim How species respond to ongoing climate change has been a hot research topic, especially with the controversy in shifting range (movement) or persisting in local habitat (in situ) as the primary response. Assessing the relative roles of range shifts, phenotypic plasticity and genetic adaptation helps us predict the evolutionary fate of species. We aim to explore the evolutionary strategies of plants under climate change from a keystone herb in alpine ecosystems, Mirabilis himalaica, along its elevational gradient. Location Himalaya‐Hengduan Mountains, China. Methods We combined evidence from population genomics and ecological data in both space and time to investigate the state of “staying” or “moving”. We identified migration events by assessing historical and contemporary gene flow and changes in species distribution. Morphological variation was compared by measuring five traits using specimen data. Moreover, we explored climate‐driven genetic variation and local selection regimes acting on populations in the alpine landscape along an elevational gradient. Results Our results argue that staying in situ by morphological variation and local genetic evolution rather than range shifting plays an important role in M. himalaica response to climate change. We first found trace evidence of upward or climatic‐driven shifting along an elevational gradient, although asymmetric gene flow was restricted within microenvironments of mid‐elevational populations. Furthermore, morphological variation comparisons revealed clinal variation, as resource allocation showed a declining pattern in vegetative growth but increased reproductive growth with increasing elevation. Outlier tests and environment association analyses indicated adaptative loci primarily related to thermal‐driven selection and continuous adaptations to high elevation in the Himalaya‐Hengduan Mountains. Main Conclusions Our findings show M. himalaica may persist in local habitats rather than shifting range under climate change, exhibiting a low risk of genomic vulnerability in current habitats. This study has important implications in improving our understanding of the evolutionary response in alpine plants to climate change.

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Section: Discussionmentioning

confidence: 99%

Staying in situ or shifting range under ongoing climate change: A case of an endemic herb in theHimalaya‐HengduanMountains across elevational gradients

Lin

Liu

Landis

et al. 2023

Diversity and Distributions

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“…Enzymatic assays of the CR4 cytoplasmic domains obtained from prokaryotic 18,[21][22][23] or eukaryotic expression hosts (Fig. 1b,c) clearly identify CR4s as active protein kinases.…”

Section: Discussionmentioning

confidence: 99%

“…Anti-sense knock-down or insertion mutation-based knock-out of ACR4, the Arabidopsis ortholog of maize CR4, again resulted in epidermis differentiation defects, leading to, for example, abnormal embryo and seed development [17][18][19] . ACR4 localizes to the plasma membrane and to endosomes [17][18][19][20] and is a catalytically active protein kinase 18,[21][22][23] . Sequence analysis of four ACR4 homologs in Arabidopsis indicated the presence of a conserved N-terminal β-propeller structure in CRINKLY4 ectodomains 21 .…”

Section: Introductionmentioning

confidence: 99%

Crystal structures of Arabidopsis and Physcomitrella CR4 reveal the molecular architecture of CRINKLY4 receptor kinases

Okuda

Hothorn

2020

Preprint

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Plant-unique receptor kinases harbor conserved cytoplasmic kinase domains and sequence-diverse ectodomains. Here we report crystal structures of CRINKLY4-type ectodomains from Arabidopsis ACR4 and Physcomitrella patens PpCR4 at 1.95 Å and 2.70 Å resolution, respectively. Monomeric CRINKLY4 ectodomains harbor a N-terminal WD40 domain and a cysteine-rich domain (CRD) connected by a short linker. The WD40 domain forms a seven-bladed β-propeller with the N-terminal strand buried in its center. Each propeller blade is stabilized by a disulfide bond. The CRD forms a β-sandwich structure stabilized by six disulfide bonds and shares low structural homology with tumor necrosis factor receptor domains. Quantitative binding assays reveal that ACR4 is not a direct receptor for the peptide hormone CLE40. An ACR4 variant lacking the entire CRD can rescue the known acr4-2 mutant phenotype, as can expression of PpCR4. Together, an evolutionary conserved signaling function for CRINKLY4 receptor kinases is encoded in its WD40 domain.

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“…The ERECTA was involved in the thermo tolerance, stomatal development, plant architecture in Arabidopsis 64 . The serine/threonine-protein kinase ACR4 targeted by LL-miR25 plays important roles in cell division and differentiation in Arabidopsis 65 . The polycomb group protein FIE2, which prevents fertilization-independent seed development in Arabidopsis 66 , is a potential LL-miR08 target.…”

Section: Discussionmentioning

confidence: 99%

Characterization of Conserved and Novel microRNAs in Lilium lancifolium Thunb. by High-Throughput Sequencing

Shenkute

Wang

et al. 2018

Sci Rep

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MicroRNAs (miRNAs) are among the class of noncoding small RNA molecules and play a crucial role in post-transcriptional regulation in plants. Although Lilium is one of the most popular ornamental flowers worldwide, however, there is no report on miRNAs identification. In the present study, therefore, miRNAs and their targets were identified from flower, leaf, bulblet and bulb of Lilium lancifolium Thunb. by high-throughput sequencing and bioinformatics analysis. In this study, a total of 38 conserved miRNAs belonging to 17 miRNA families and 44 novel miRNAs were identified. In total, 366 target genes for conserved miRNAs and 415 target genes for novel miRNAs were predicted. The majority of the target genes for conserved miRNAs were transcriptional factors and novel miRNAs targeted mainly protein coding genes. A total of 53 cleavage sites belonging to 6 conserved miRNAs families and 14 novel miRNAs were identified using degradome sequencing. Twenty-three miRNAs were randomly selected, then, their credibility was confirmed using northern blot or stem-loop qRT-PCR. The results from qRT-PCR analysis showed the expression pattern of 4 LL-miRNAs was opposite to their targets. Therefore, our finding provides an important basis to understand the biological functions of miRNAs in Lilium.

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Characterization and enzymatic properties of protein kinase ACR4 from Arabidopsis thaliana

Cited by 4 publications

References 20 publications

Staying in situ or shifting range under ongoing climate change: A case of an endemic herb in theHimalaya‐HengduanMountains across elevational gradients

Staying in situ or shifting range under ongoing climate change: A case of an endemic herb in theHimalaya‐HengduanMountains across elevational gradients

Crystal structures of Arabidopsis and Physcomitrella CR4 reveal the molecular architecture of CRINKLY4 receptor kinases

Characterization of Conserved and Novel microRNAs in Lilium lancifolium Thunb. by High-Throughput Sequencing

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