Characterization and enzymic degradation of sugar beet fibres

Rouau, Xavier; Bertin, Chantal; Thibault, Jean‐François

doi:10.1016/s0268-005x(87)80037-1

Cited by 6 publications

(4 citation statements)

References 10 publications

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“…Increased swelling and WBC have been described previously for pellets of alkali-extracted beet fibers [ 36 , 37 ], even though these fibers had strongly reduced levels of pectic arabinan and galacturonan. Analogously, it has been reported for the cell-wall material of wheat flour that enzymatic removal of arabinoxylan increased the WBC of the residual, cellulose-rich material [ 38 ].…”

Section: Resultsmentioning

confidence: 56%

“…Analogously, it has been reported for the cell-wall material of wheat flour that enzymatic removal of arabinoxylan increased the WBC of the residual, cellulose-rich material [ 38 ]. Possible explanations for the increased water binding include the total polysaccharide content, charge, crystallinity of the cellulose, specific surface area, and hydrophobic components [ 37 ].…”

Section: Resultsmentioning

confidence: 99%

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Aiming for the complete utilization of sugar-beet pulp: Examination of the effects of mild acid and hydrothermal pretreatment followed by enzymatic digestion

Kühnel

Schols

Gruppen

2011

Biotechnol Biofuels

103

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BackgroundBiomass use for the production of bioethanol or platform chemicals requires efficient breakdown of biomass to fermentable monosaccharides. Lignocellulosic feedstocks often require physicochemical pretreatment before enzymatic hydrolysis can begin. The optimal pretreatment can be different for different feedstocks, and should not lead to biomass destruction or formation of toxic products.MethodsWe examined the influence of six mild sulfuric acid or water pretreatments at different temperatures on the enzymatic degradability of sugar-beet pulp (SBP).ResultsWe found that optimal pretreatment at 140°C of 15 minutes in water was able to solubilize 60% w/w of the total carbohydrates present, mainly pectins. More severe treatments led to the destruction of the solubilized sugars, and the subsequent production of the sugar-degradation products furfural, hydroxymethylfurfural, acetic acid and formic acid. The pretreated samples were successfully degraded enzymatically with an experimental cellulase preparation.ConclusionsIn this study, we found that pretreatment of SBP greatly facilitated the subsequent enzymatic degradation within economically feasible time ranges and enzyme levels. In addition, pretreatment of SBP can be useful to fractionate functional ingredients such as arabinans and pectins from cellulose. We found that the optimal combined severity factor to enhance the enzymatic degradation of SBP was between log R'0 = -2.0 and log R'0 = -1.5. The optimal pretreatment and enzyme treatment solubilized up to 80% of all sugars present in the SBP, including ≥90% of the cellulose.

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Section: Resultsmentioning

confidence: 56%

Section: Resultsmentioning

confidence: 99%

Aiming for the complete utilization of sugar-beet pulp: Examination of the effects of mild acid and hydrothermal pretreatment followed by enzymatic digestion

Kühnel

Schols

Gruppen

2011

Biotechnol Biofuels

103

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“…The determined sugars were D-glucose (Glu), D-galacturonic acid (D-GalA), L-arabinose (Ara), D-galactose (Gal), D-xylose (Xyl), D-mannose (Man), L-rhamnose (Rha), L-fucose (Fuc) and D-glucuronic acid (GluA), and residual mass (RM). The results are compared to the literature and their mean values of the respective sugars [13,54,55]. The differences in reported data from other studies are likely due to a large extent to the biological variance of the raw material.…”

Section: Composition Of Sbppmentioning

confidence: 91%

Enzymatic One-Pot Hydrolysis of Extracted Sugar Beet Press Pulp after Solid-State Fermentation with an Engineered Aspergillus niger Strain

Knesebeck,

Schäfer,

Schmitz

et al. 2023

Fermentation

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Extracted sugar beet press pulp (SBPP) is a promising agricultural residue for saccharification and further bioconversion. Combining solid-state fermentation of SBPP with engineered Aspergillus niger for enzyme production followed by hydrolysis of additionally added SBPP in the same bioreactor was studied to produce a sugar solution (hydrolysate) in a one-pot process. The initial aerobic solid-state fermentations were carried out in duplicate on non-milled, wet SBPP (moisture content of 72% (w/v)) with an A. niger strain engineered for constitutive pectinase production for 96 h, and this resulted in polygalacturonase activities of up to 256 U mL−1 in the wet media. Afterwards, water was added to the bioreactor, and the remaining solids were suspended by stirring to dissolve the hydrolytic enzymes. Metabolic activities of A. niger were inactivated by a N2-atmosphere and by increasing the temperature to 50 °C. High solid loads of milled SBPP were added to the stirred-tank reactor with a delay of 24 h to enable sugar yield calculations based on the compositional analysis of the SBPP used. The resulting final sugar concentrations of the hydrolysate after 166 h were 17 g L−1 d-glucose, 18.8 g L−1 l-arabinose, and 12.5 g L−1 d-galacturonic acid, corresponding to sugar yields of 98% d-glucose, 86% l-arabinose, and 50% d-galacturonic acid, respectively. Including the other sugars released during enzymatic hydrolysis in the one-pot process (d-xylose, d-mannose, d-galactose), a total sugar concentration of 54.8 g L−1 was achieved in the hydrolysate. The one-pot process combining hydrolytic enzyme production in solid-state fermentation with high solid loads during enzymatic hydrolysis of the milled SBPP reduces hydrolytic process costs by replacing chemical pre-treatments, enabling the in situ production of SBPP-adapted hydrolytic enzymes, as well as avoiding intermediate enzyme extraction and preparation steps.

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“…An almost total degradation of this structure can be obtained by the use of a mixture of pectinolytic and cellulolytic activities. The degradation of cell walls by such mixtures was already studied for sugar beet pulp (2)(3)(4)(5), carrot (6)(7)(8)(9), potato (2) and apple (10,11). These studies described mainly the results of the simultaneous action of pectinases and cellulases, and only few reports were concerned with the degradation of material obtained after the action of pectinolytic enzymes (6,7).…”

Section: Introductionmentioning

confidence: 99%

Influence of Pretreatments on Enzymic Degradation of a Cellulose-rich Residue from Sugar-beet Pulp

Micard¹,

Renard²,

Thibault³

1997

LWT - Food Science and Technology

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Characterization and enzymic degradation of sugar beet fibres

Cited by 6 publications

References 10 publications

Aiming for the complete utilization of sugar-beet pulp: Examination of the effects of mild acid and hydrothermal pretreatment followed by enzymatic digestion

Aiming for the complete utilization of sugar-beet pulp: Examination of the effects of mild acid and hydrothermal pretreatment followed by enzymatic digestion

Enzymatic One-Pot Hydrolysis of Extracted Sugar Beet Press Pulp after Solid-State Fermentation with an Engineered Aspergillus niger Strain

Influence of Pretreatments on Enzymic Degradation of a Cellulose-rich Residue from Sugar-beet Pulp

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