Characterization and Evaluation of Antibacterial Activities of Chemically Synthesized Iron Oxide Nanoparticles

Abstract

The iron oxide nanoparticles have been synthesized in co-precipitation method using aqueous solution of ferric and ferrous ions with sodium salt. The synthesis of iron-oxide nanoparticles were validated by UV-Visible spectroscopy which showed higher peak at 370 nm as valid standard reference. An average size of iron oxide nanoparticle found by Diffraction Light scattering (DLS) particle size analyser, ranges approximately between 10 nm to 120 nm with mean particle size of 66 nm. The X-ray power diffraction (XRD) analysis revealed the crystallographic structure of magnetic particles. Characterization of the mean particle size and morphology of iron oxide nanoparticles confirmed that the iron oxide nanoparticles are nearly spherical and crystalline in shape. Further the antibacterial effect of iron oxide nanoparticles was evaluated against ten pathogenic bacteria which showed that the nanoparticles have moderate antibacterial activity against both Gram positive and Gram negative pathogenic bacterial strains and retains potential application in pharmaceutical and biomedical industries

Synthesis and Characterization of Fe3O4 Nanoparticles Modified with Polyethylene Glycol as Antibacterial Material

“…One Tof the magnetic nanoparticles is Fe 3 O 4 (magnetite). The materials exhibit superparamagnetic behavior, low toxicity, biocompatibility, easier surface modification [13,14].…”

“…The Fe 3 O 4 nanoparticle has the greatest antibacterial effect to pathogenic bacteria of Pseudomonas aeroginosa than Escherichia coli and Staphylococcus aureus [15]. In addition, Fe 3 O 4 nanoparticle has a zone of inhibition consideration to Ag nanoparticle for topical use [14]. Materials in the form of small particle sizes are easy to agglomerate and made it larger particle size, reducing surface area and magnetic properties.…”

Magnetite Nanostructures as Novel Strategies for Anti-Infectious Therapy

“…Viability, growth Pseudomonas aeruginosa [26] MNPs/Ag/chitosan Viability Pseudomonas aeruginosa [39] MNPs stabilized with thioglycerol Viability, growth Pseudomonas aeruginosa [45] MNPs coated with vancomycin, penicillin and streptomycin Adherence, biofilm formation Enterococcus faecalis [47] MNPs/Ag/chitosan Viability, growth Enterococcus faecalis [39] dextran MNPs coated with amino acids (L-arginine and L-lysine) Viability, growth Listeria monocytogenes [49] MNPs stabilized with thioglycerol Viability, growth Bacilus subtilis [45] 66 nm of iron oxide MNPs Viability, growth Bacilus subtilis [48] 66 nm of iron oxide MNPs Viability, growth Bacilus licheniformis [48] 66 nm of iron oxide MNPs Viability, growth Bacilus brevis [48] 66 nm of iron oxide MNPs Viability, growth Vibrio cholerae [48] 66 nm of iron oxide MNPs Viability, growth Streptococcus aureus [48] An example of the mechanism by which MNPs functionalized with antibiotics improve the antimicrobial inhibition is illustrated in Figure 3. Like the antibiotics which act by interrupting the cell wall synthesis (Figure 3a), studies have revealed that magnetite nanoparticles coated with a chitosan polymeric matrix are able to also bind to bacterial cell walls and interrupt its synthesis.…”

“…Viability, growth Staphylococcus aureus [26] MNPs/Ag/chitosan Viability, growth Staphylococcus aureus [39] MNPs stabilized with thioglycerol Viability, growth Staphylococcus aureus [45] 66 nm of iron oxide MNPs Viability, growth Staphylococcus aureus [48] MNPs/chitosan/ aminoglycosides (kanamycin and neomycin) Viability, growth Pseudomonas aeruginosa [27] Spherical MNPs coated with eugenol Adherence, biofilm formation Pseudomonas aeruginosa [46] MNPs/chitosan/carboxymethylcellulose/ antibiotics (penicillins, macrolides, aminoglycosides, rifampicines quinolones)…”

Synthesis of iron oxide nanoparticles in a continuous flow spiral microreactor and Corning® advanced flow™ reactor

“…Yuvakkumar et al [24] prepared nano-scaled zero valent iron (50−100 nm) using the reduction method based on the use of ferric ions and sodium borohydride in ethanol under atmospheric conditions. Behera et al [25] also prepared iron oxide NPs for antibacterial applications using co-precipitation method. Lee et al [26] investigated the synthesis of iron oxide nanomaterials in microfluidics device under ambient conditions with the use of co-precipitation method.…”