Characterization and expression analysis of FGF6 (fibroblast growth factor 6) genes of grass carp (Ctenopharyngodon idellus) reveal their regulation on muscle growth

Xu, Yingyan; Tan, Qingsong; Hu, Pengcheng; Yao, Junpeng

doi:10.1007/s10695-019-00655-0

Cited by 13 publications

(10 citation statements)

References 46 publications

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“…Meanwhile, the mrf4 linearly decreased as the dietary protein level increased, which may be explained by the mrf4 acted as a negative regulator of fiber hypertrophy as demonstrated by Moretti et al ( 29 ) using an RNA interference (RNAi) approach. In addition, fgf6a and mstn possibly play a crucial role in the regulation of muscle proliferation and hypertrophy in fish ( 30 , 31 ), which agree with the mstn and fgf6a expression level responding to the muscle growth in this study.…”

Section: Discussionsupporting

confidence: 89%

Growth and Meat Quality of Grass Carp (Ctenopharyngodon idellus) Responded to Dietary Protein (Soybean Meal) Level Through the Muscle Metabolism and Gene Expression of Myosin Heavy Chains

et al. 2022

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The aim of this study was to investigate the effect of dietary protein level (soybean meal) on growth performance, flesh quality of grass carp, and the related molecular mechanisms. The results showed that appropriate dietary protein levels improved the growth performance, hardness, and pH of muscle while decreasing muscle crude lipid content and cooking loss and altering the antioxidant capacity and metabolic enzymes activities. In addition, appropriate dietary protein promoted the gene expression of myhc-1, myhc-4, myf5, myod, myog, and fgf6a, whereas inhibited that of myhc-7, myhc-2, mrf4, and mstn. Transcriptome profiling of muscle revealed that the flesh quality-specific differences were related to tight junctions and intramuscular fat (IMF) accumulation. GSEA showed that fatty acid metabolism and oxidative phosphorylation were downregulated in SM5 compared with SM1. To conclude, appropriate protein levels improved the growth and flesh quality by regulating muscle antioxidant capacity and gene expression of myhcs and fat metabolism-related signaling molecules.

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Section: Discussionsupporting

confidence: 89%

Growth and Meat Quality of Grass Carp (Ctenopharyngodon idellus) Responded to Dietary Protein (Soybean Meal) Level Through the Muscle Metabolism and Gene Expression of Myosin Heavy Chains

et al. 2022

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“…Based on the relationship between muscle cellularity and fgf6 s expression, this might suggest that the dietary DDGS mainly acts on the expression of fgf6a rather than fgf6b to regulate hypertrophy thus to improve fish growth, while fgf6b might be involved in hyperplasia. These results partially agree with (Abouel Azm et al, 2021; Campos et al, 2010; Xu et al, 2019), however, Xu et al, (2019) also suggested that fgf6b possibly had a role in muscle hypertrophic growth regulation. This differentiation deserves further confirmation.…”

Section: Discussionsupporting

confidence: 86%

“…The increase in gene expression of MRFs and mstn1 in the control group reflects the higher potential for muscle regeneration in this group, which indicates that fish body produces more muscle cells to compensate the growth depression caused by the anti‐nutritional factors such as gossypol in CSM. This could be confirmed by previous studies (Aksnes et al, 1986; Hu et al, 2019; Ulloa et al, 2013; Xu et al, 2019; Zhao et al, 2018), all of which suggested that muscle regeneration occurred in growth‐restricted fish. Upregulation of mstn gene expression in the control group compared with DDGS containing groups indicated that mstn may prevent the hypertrophy of fish muscle cells rather than differentiation, that confirmed the results by Lee and McPherron (2001).…”

Section: Discussionsupporting

confidence: 84%

Effects of replacement of dietary cottonseed meal by distiller’s dried grains with solubles on growth performance, muscle texture, health and expression of muscle‐related genes in grass carp ( Ctenopharyngodon idellus )

et al. 2021

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Both cottonseed meal (CSM) and dried distiller's grains with solubles (DDGS) are important plant protein sources for fish. This study investigated the effects of dietary DDGS inclusion to replace different portions of CSM on growth performance, muscle texture, health status as well as myogenic‐related genes expression of juvenile grass carp (initial weight: 5 g). A 60‐d feeding trial was performed by feeding fish with three isonitrogenous and isoenergetic diets containing different levels of DDGS (0, 10.3% and 20.6%). The highest growth and feed efficiency occurred at 20.6% DDGS dietary inclusion. Body condition factor, hepatosomatic index, serum ALT and AST as well as hepatic GSH and T‐SOD all showed a downward trend with increasing dietary inclusion of DDGS (p < .05). Collagen content in raw muscle increased with DDGS inclusion. However, DDGS inclusion decreased muscle texture indices and muscle fibre density, while increased muscle fibre diameter (p < .05). Dietary DDGS inclusions significantly upregulated the expression of fgf6a, myh1, myh2 and myh4 in muscle, while downregulated the expression of myod, myf5, myog, mrf4, mstn1, fgf6b and myh7. Overall, dietary DDGS replacing CSM positively affects growth by regulating gene expression involved in myogenesis and hypertrophy, while depressed antioxidant capacity.

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“…Notably, native DDGS mainly act on the expression of fgf6a rather than fgf6b to regulate hyperplasia in this study. These two genes have different expression profiles in our study, in which the lower expression of fgf6a in US-imported DDGS groups was positively correlated to the muscle fiber density, while fgf6b expression showed an unsteady trend, although it was suggested that fgf6b had a function in muscle hypertrophy growth regulation ( 58 ). These distinctions in the function of fgf6 subtypes deserve more confirmation.…”

Section: Discussionmentioning

confidence: 72%

The Interaction of Dried Distillers Grains With Solubles (DDGS) Type and Level on Growth Performance, Health, Texture, and Muscle-Related Gene Expression in Grass Carp (Ctenopharyngodon idellus)

et al. 2022

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The aim of this study was to estimate the possible synergetic effects of the two levels of dietary dried distillers grains with solubles (DDGS) from different sources (US-imported and native) on the growth, health status, muscle texture, and muscle growth-related gene expression of juvenile grass carp. Four treatments of fish were fed with 4 isonitrogenous diets, namely, native DDGS20, native DDGS30, US-imported DDGS20, and US-imported DDGS30 for 60 days. The US-imported DDGS30 group showed the better growth and feed efficiency. Additionally, we observed a significant increase in hepatopancreatic total antioxidant capacity (T-AOC) and superoxide dismutase (SOD) in native DDGS groups. Moreover, raw muscle collagen increases considerably in the US-imported DDGS30 compared with the native DDGS30 group. In comparison with the native DDGS groups, the US-imported DDGS groups showed significantly decrease in all textural properties and fiber density, while increased fiber diameter. Dietary native DDGS inclusion significantly showed the upregulation of myog, myhc, and fgf6a expression in muscle, while the downregulation of the expression of myod and myf5. Overall, US-imported DDGS30 had a beneficial influence on growth via regulating genes involved in myogenesis and hypertrophy, the formation of collagen, but had negative impacts on antioxidant capacity and cooked muscle texture.

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Characterization and expression analysis of FGF6 (fibroblast growth factor 6) genes of grass carp (Ctenopharyngodon idellus) reveal their regulation on muscle growth

Cited by 13 publications

References 46 publications

Growth and Meat Quality of Grass Carp (Ctenopharyngodon idellus) Responded to Dietary Protein (Soybean Meal) Level Through the Muscle Metabolism and Gene Expression of Myosin Heavy Chains

Growth and Meat Quality of Grass Carp (Ctenopharyngodon idellus) Responded to Dietary Protein (Soybean Meal) Level Through the Muscle Metabolism and Gene Expression of Myosin Heavy Chains

Effects of replacement of dietary cottonseed meal by distiller’s dried grains with solubles on growth performance, muscle texture, health and expression of muscle‐related genes in grass carp ( Ctenopharyngodon idellus )

The Interaction of Dried Distillers Grains With Solubles (DDGS) Type and Level on Growth Performance, Health, Texture, and Muscle-Related Gene Expression in Grass Carp (Ctenopharyngodon idellus)

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