Characterization and expression analysis of P5CS (Δ1-pyrroline-5-carboxylate synthase) gene in two distinct populations of the Atlantic Forest native species Eugenia uniflora L.

Anton, Débora Bublitz; Guzmán, Frank; Vetö, Nicole Moreira; Krause, Felipe Augusto; Kulcheski, Franceli Rodrigues; Coelho, Ana Paula Durand; Duarte, Guilherme Coutinho Kullmann; Margis, Rogério; Dillenburg, Lúcia Rebello; Turchetto‐Zolet, Andreia Carina

doi:10.1007/s11033-019-05195-7

Cited by 11 publications

(8 citation statements)

References 49 publications

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“…6 ). This was further evaluated and confirmed by the expression profile of pyrroline-5-carboxyalate synthase (P5CS) , a gene responsible for Pro biosynthesis 75 , 76 . The primed plants showed an upregulation of P5CS exhibiting 8.2- and 2.6-fold increase under MD and SD, respectively (Fig.…”

Section: Resultsmentioning

confidence: 91%

Molecular mechanisms associated with microbial biostimulant-mediated growth enhancement, priming and drought stress tolerance in maize plants

Lephatsi

Nephali

Meyer

et al. 2022

Sci Rep

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Microbial-based biostimulants are emerging as effective strategies to improve agricultural productivity; however, the modes of action of such formulations are still largely unknown. Thus, herein we report elucidated metabolic reconfigurations in maize (Zea mays) leaves associated with growth promotion and drought stress tolerance induced by a microbial-based biostimulant, a Bacillus consortium. Morphophysiological measurements revealed that the biostimulant induced a significant increase in biomass and enzymatic regulators of oxidative stress. Furthermore, the targeted metabolomics approach revealed differential quantitative profiles in amino acid-, phytohormone-, flavonoid- and phenolic acid levels in plants treated with the biostimulant under well-watered, mild, and severe drought stress conditions. These metabolic alterations were complemented with gene expression and global DNA methylation profiles. Thus, the postulated framework, describing biostimulant-induced metabolic events in maize plants, provides actionable knowledge necessary for industries and farmers to confidently and innovatively explore, design and fully implement microbial-based formulations and strategies into agronomic practices for sustainable agriculture and food production.

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Section: Resultsmentioning

confidence: 91%

Molecular mechanisms associated with microbial biostimulant-mediated growth enhancement, priming and drought stress tolerance in maize plants

Lephatsi

Nephali

Meyer

et al. 2022

Sci Rep

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“…It is interesting as the antioxidant ability of proline by being an oxygen quencher under stress has been acknowledged ( Szabados and Savouré, 2010 ). In particular, the considerably increased expression of P5CS transcript (>8-folds) reflected it as an important candidate to understand the processes of abiotic stress tolerance in several plants ( Maghsoudi et al, 2018 ; Anton et al, 2020 ). Together these results suggest that better performance of P. antidotale to low salinity was related to consistently up-regulated metabolism (transcripts and proteins) for key amino acids i.e., proline and methionine; the high salinity treatment altered the functional elucidation (protein abundance) of upregulated transcripts of various amino acids, but lysine and arginine mainly contributed to the osmotic tolerance of these plants.…”

Section: Discussionmentioning

confidence: 99%

Combined Transcriptome and Proteome Analysis to Elucidate Salt Tolerance Strategies of the Halophyte Panicum antidotale Retz

et al. 2021

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Panicum antidotale, a C4 monocot, has the potential to reclaim saline and drylands and to be utilized as fodder and forage. Its adaptability to survive saline stress has been proven with eco-physiological and biochemical studies. However, little is known about its molecular mechanisms of salt tolerance. In this study, an integrated transcriptome and proteome analysis approach, based on RNA sequencing and liquid chromatography tandem mass spectrometry (LC-MS/MS), was used to identify the said mechanisms. Plants were treated with control (0 mM), low (100 mM), and high (300 mM) sodium chloride (NaCl) treatments to distinguish beneficial and toxic pathways influencing plant biomass. The results indicated differential expression of 3,179 (1,126 upregulated/2,053 downregulated) and 2,172 (898 upregulated/1,274 downregulated) genes (DEGs), and 514 (269 upregulated/245 downregulated) and 836 (494 upregulated/392 downregulated) proteins (DEPs) at 100 and 300 mM NaCl, respectively. Among these, most upregulated genes and proteins were involved in salt resistance strategies such as proline biosynthesis, the antioxidant defense system, ion homeostasis, and sugar accumulation at low salinity levels. On the other hand, the expression of several genes and proteins involved in the respiratory process were downregulated, indicating the inability of plants to meet their energy demands at high salinity levels. Moreover, the impairments in photosynthesis were also evident with the reduced expression of genes regulating the structure of photosystems and increased expression of abscisic acid (ABA) mediated pathways which limits stomatal gas exchange. Similarly, the disturbance in fatty acid metabolism and activation of essential ion transport blockers damaged the integrity of the cell membrane, which was also evident with enhanced malondialdehyde (MDA). Overall, the analysis of pathways revealed that the plant optimal performance at low salinity was related to enhanced metabolism, antioxidative defense, cell growth, and signaling pathways, whereas high salinity inhibited biomass accumulation by altered expression of numerous genes involved in carbon metabolism, signaling, transcription, and translation. The data provided the first global analysis of the mechanisms imparting salt stress tolerance of any halophyte at transcriptome and proteome levels.

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“…P5CS is a key enzyme in the first two steps of proline biosynthesis and has been isolated from many plants so far (Garg and Neha, 2019). A previous study showed a correlation between the infusion of P5CS gene and the proline accumulation in Arabidopsis thaliana (Yoshiba et al ., 1995; Signorelli and Monza, 2017; Anton et al ., 2020). Another study showed that P5CS gene expression, under salinity stress on days 9 and 11 of treatment with NaCl, was enhanced in cactus pear (Silva-Ortega et al ., 2008).…”

Section: Discussionmentioning

confidence: 99%

Effects of salinity stress on proline content and expression ofΔ¹-pyrroline-5-carboxylate synthaseandvacuolar-type H⁺subunit Egenes in wheat

Goharrizi

Baghizadeh

Afroushteh

et al. 2020

Plant Genet. Resour.

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To evaluate the responses of some important agronomic traits of 14 bread wheat cultivars, a split plot experiment was conducted based on a randomized complete block design under non-stress, moderate and severe salinity stress conditions. In this study, Backcross and Roshan were identified as the most salinity-tolerant cultivars, while Mihan and Shirudi were considered as the most salinity-sensitive cultivars. The proline content, as well as Δ1-pyrroline-5-carboxylate synthase (P5CS) and vacuolar-type H+-ATPase subunit E (W36) gene expression levels, were examined in these cultivars under normal, moderate and high salinity stress conditions. The proline content and P5CS gene expression level increased with a rise in NaCl concentration. Further, a direct relationship was observed between the proline content and P5CS gene expression in all samples. Our results showed that W36 gene expression in Backcross and Roshan cultivars, as the most resistant ones, strongly increased with elevation of the NaCl concentrations. On the other hand, in the sensitive cultivars such as Mihan and Shirudi, small changes were observed in the gene expression levels with rising salinity levels. Additionally, Backcross and Roshan cultivars had the highest proline content as well as P5CS and W36 genes expression levels under moderate and high salinity stress levels.

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Characterization and expression analysis of P5CS (Δ1-pyrroline-5-carboxylate synthase) gene in two distinct populations of the Atlantic Forest native species Eugenia uniflora L.

Cited by 11 publications

References 49 publications

Molecular mechanisms associated with microbial biostimulant-mediated growth enhancement, priming and drought stress tolerance in maize plants

Molecular mechanisms associated with microbial biostimulant-mediated growth enhancement, priming and drought stress tolerance in maize plants

Combined Transcriptome and Proteome Analysis to Elucidate Salt Tolerance Strategies of the Halophyte Panicum antidotale Retz

Effects of salinity stress on proline content and expression ofΔ¹-pyrroline-5-carboxylate synthaseandvacuolar-type H⁺subunit Egenes in wheat

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Characterization and expression analysis of P5CS (Δ1-pyrroline-5-carboxylate synthase) gene in two distinct populations of the Atlantic Forest native species Eugenia uniflora L.

Cited by 11 publications

References 49 publications

Molecular mechanisms associated with microbial biostimulant-mediated growth enhancement, priming and drought stress tolerance in maize plants

Molecular mechanisms associated with microbial biostimulant-mediated growth enhancement, priming and drought stress tolerance in maize plants

Combined Transcriptome and Proteome Analysis to Elucidate Salt Tolerance Strategies of the Halophyte Panicum antidotale Retz

Effects of salinity stress on proline content and expression ofΔ1-pyrroline-5-carboxylate synthaseandvacuolar-type H+subunit Egenes in wheat

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Effects of salinity stress on proline content and expression ofΔ¹-pyrroline-5-carboxylate synthaseandvacuolar-type H⁺subunit Egenes in wheat