Characterization and expression of NADPH oxidase in LPS-, poly(I:C)- and zymosan-stimulated trout (Oncorhynchus mykiss W.) macrophages

Boltaña, Sebastián; Doñate, Carmen; Goetz, Frederick W.; Mackenzie, Simon; Balasch, Juan

doi:10.1016/j.fsi.2008.11.011

Cited by 22 publications

(16 citation statements)

References 63 publications

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“…Regarding LPS exposure, and in line with the present findings, Boltaña et al (2009) [39] obtained an increased expression of NADPH oxidase system mRNAs in rainbow trout HKM stimulated with 10 mg/ml LPS for 12 h. In our study the differences between the effects caused by copper or LPS alone and those obtained after exposure to the mixture, revealed an antagonistic interaction, possibly due to a cumulative effect of both stimulus and subsequent ROS overproduction, impairing cell metabolism.…”

Section: Discussionsupporting

confidence: 90%

Gene expression and TNF-alpha secretion profile in rainbow trout macrophages following exposures to copper and bacterial lipopolysaccharide

Mackenzie

Boltaña

Callol

et al. 2011

Fish & Shellfish Immunology

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Section: Discussionsupporting

confidence: 90%

Gene expression and TNF-alpha secretion profile in rainbow trout macrophages following exposures to copper and bacterial lipopolysaccharide

Mackenzie

Boltaña

Callol

et al. 2011

Fish & Shellfish Immunology

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“…The dietary supplementation of another Euglenoid species (Euglena viridis) in the diet for rohu (Labeo rohita) also enhanced the production of reactive oxygen species and likely was related to the paramylon content in this microalgae (Das, Pradhan, & Sahu, 2009). Corroborating the present results, zymosan also triggered an increased gene expression of NADPH oxidase in vitro when incubated with rainbow trout (Oncorhynchus mykiss) macrophages (Boltana, Doñate, Goetz, MacKenzie, & Balasch, 2009), and respiratory burst and inflammatory gene expression of carp (Cyprinus carpio) macrophages was enhanced (Pietretti, Vera-Jimenez, Hoole, & Wiegertjes, 2013). Corroborating the present results, zymosan also triggered an increased gene expression of NADPH oxidase in vitro when incubated with rainbow trout (Oncorhynchus mykiss) macrophages (Boltana, Doñate, Goetz, MacKenzie, & Balasch, 2009), and respiratory burst and inflammatory gene expression of carp (Cyprinus carpio) macrophages was enhanced (Pietretti, Vera-Jimenez, Hoole, & Wiegertjes, 2013).…”

Section: Ta B L Esupporting

confidence: 80%

Immunomodulatory effects of β‐glucans derived from Euglena gracilis or Saccharomyces cerevisiae for hybrid striped bass ( Morone chrysops × M. saxatilis )

Yamamoto

Castillo²,

Gatlin

2019

Aquac Res

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Beta-glucans (BGs) can activate the animal's innate immune system, enhancing the primary defence lines against pathogenic insults. The objective of this study was to assess the immune responses of hybrid striped bass (HSB) when exposed to paramylon, derived from Euglena gracilis, or zymosan, derived from Saccharomyces cerevisiae. Two separate trials were conducted to evaluate the efficacy of these immunostimulants as a feed additive or injected intraperitoneally. Five diets were formulated to contain either paramylon or zymosan at two different levels (50 or 100 mg/kg), and no supplementation serving as the control. Blood and head kidney were sampled, and an elevated production of anion superoxide from isolated phagocytes was observed for all supplemented groups when compared to the control. Dietary paramylon at 50 mg/ kg increased immunoglobulin levels in the plasma. The second trial was conducted by injecting a BG solution (10 mg of β-glucan kg -1 of body weight) and phosphate buffer solution serving as the control. Seven days after injection, blood samples were collected and immunological profiles from whole blood and plasma were significantly (p < .05) affected by the treatments. The results from this study indicate that both dietary and injected paramylon and zymosan modulated the immunological responses of HSB.

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“…Furthermore, virally infected fish (Lockhart et al, 2007;McBeath et al, 2007) and poly (I:C)-treated fish expressed gene transcripts for type 1 IFNs and Mx protein expression (Arnemo et al, 2014;Das et al, 2009;Lockhart et al, 2004a;McBeath et al, 2007). At the cellular level, repeated poly (I:C) stimulation enhanced ROS production of HK macrophages in O. mykiss (Boltaña et al, 2009). Furthermore, ifnγ induced nitric oxide (NO) production to facilitate intracellular pathogen destruction (Stafford et al, 1999), and ifnγ enhanced respiratory burst, phagocytosis and intracellular NO production in vitro (Grayfer & Belosevic, 2009) and in vivo (Zhou et al, 2014).…”

Section: Introductionmentioning

confidence: 98%

Metabolic and immune responses of Chinook salmon (Oncorhynchus tshawytscha) smolts to a short‐term poly (I:C) challenge

Lulijwa

Mérien

Burdass

et al. 2020

Journal of Fish Biology

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Polyinosinic:polycytidylic acid [poly (I:C)] was administered in vivo to Chinook salmon (Oncorhynchus tshawytscha) post‐smolts to determine the immune responses on haematological and cellular functional parameters, including spleen (SP), head kidney (HK) and red blood cell (RBC) cytokine expression, as well as serum metabolomics. Poly (I:C) in vivo (24 h exposure) did not affect fish haematological parameters, leucocyte phagocytic activity and phagocytic index, reactive oxygen species and nitric oxide production. Gas chromatography–mass spectrometry–based metabolomics revealed that poly (I:C) significantly altered the serum biochemistry profile of 25 metabolites. Metabolites involved in the branched‐chain amino acid/glutathione and transsulphuration pathways and phospholipid metabolism accumulated in poly (I:C)–treated fish, whereas those involved in the glycolytic and energy metabolism pathways were downregulated. At cytokine transcript level, poly (I:C) induced a significant upregulation of antiviral ifnγ in HK and Mx1 protein in HK, SP and RBCs. This study provides evidence for poly (I:C)–induced, immune‐related biomarkers at metabolic and molecular levels in farmed O. tshawytscha in vivo. These findings provide insights into short‐term effects of poly (I:C) at haematological, innate and adaptive immunity and metabolic levels, setting the stage for future studies.

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Characterization and expression of NADPH oxidase in LPS-, poly(I:C)- and zymosan-stimulated trout (Oncorhynchus mykiss W.) macrophages

Cited by 22 publications

References 63 publications

Gene expression and TNF-alpha secretion profile in rainbow trout macrophages following exposures to copper and bacterial lipopolysaccharide

Gene expression and TNF-alpha secretion profile in rainbow trout macrophages following exposures to copper and bacterial lipopolysaccharide

Immunomodulatory effects of β‐glucans derived from Euglena gracilis or Saccharomyces cerevisiae for hybrid striped bass ( Morone chrysops × M. saxatilis )

Metabolic and immune responses of Chinook salmon (Oncorhynchus tshawytscha) smolts to a short‐term poly (I:C) challenge

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