Characterization and expression profiling of PIN auxin efflux transporters reveal their role in developmental and abiotic stress conditions in rice

Manna, Mrinalini; Balakrishnan, Rengasamy; Ambasht, Navin K.; Sinha, Alok

doi:10.3389/fpls.2022.1059559

Cited by 9 publications

(17 citation statements)

References 83 publications

(102 reference statements)

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“…Auxin is typically synthesized in axillary buds and leaf primordia and differentially distributed throughout the plant tissues by PIN family proteins that act as efflux carriers of auxin ( Sieberer and Leyser 2006 ; Swarup and Péret 2012 ). Depending on their polar localization, PINs ensure differential distribution of auxin that is vital for diverse developmental processes, including embryogenesis, tissue differentiation and organogenesis through upward, sideward, and downward transport ( Sieberer and Leyser 2006 ; Nguyen et al ., 2018 ; Manna et al ., 2022 ). In this study, the expression of PIN9 was found to be significantly up-regulated and the difference was 2.2-fold ( Fig.…”

Section: Discussionmentioning

confidence: 99%

Humic acid improves wheat growth by modulating auxin and cytokinin biosynthesis pathways

Rathor,

Upadhyay,

Ullah

et al. 2024

AoB PLANTS

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Humic acids have been widely used for centuries to enhance plant growth and productivity. The beneficial effects of humic acids have been attributed to different functional groups and phytohormone-like compounds enclosed in macrostructure. However, the mechanisms underlying the plant growth-promoting effects of humic acids are only partially understood. We hypothesize that the bio-stimulatory effect of humic acids is mainly due to the modulation of innate pathways of auxin and cytokinin biosynthesis in treated plants. A physiological investigation along with molecular characterization was carried out to understand the mechanism of bio-stimulatory effects of humic acid. A gene expression analysis was performed for the genes involved in auxin and cytokinin biosynthesis pathways in wheat seedlings. Furthermore, Arabidopsis thaliana transgenic lines generated by fusing the auxin-responsive DR5 and cytokinin-responsive ARR5 promoter to ß-glucuronidase (GUS) reporter were used to study the GUS expression analysis in humic acid treated seedlings. This study demonstrates that humic acid treatment improved the shoot and root growth of wheat seedlings. The expression of several genes involved in auxin (Tryptophan Aminotransferase of Arabidopsis and Gretchen Hagen 3.2) and cytokinin (Lonely Guy3) biosynthesis pathways was up-regulated in humic acid treated seedlings compared to the control. Furthermore, GUS expression analysis showed that bioactive compounds of humic acid stimulate endogenous auxin and cytokinin-like activities. This study is the first report in which using ARR5 :GUS lines we demonstrate the biostimulants activity of humic acid.

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Section: Discussionmentioning

confidence: 99%

Humic acid improves wheat growth by modulating auxin and cytokinin biosynthesis pathways

Rathor,

Upadhyay,

Ullah

et al. 2024

AoB PLANTS

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“…Previous studies demonstrated that OsPIN9 functions in polar auxin transport and is involved in regulating adventitious root number and tiller number [55]. Recently, Manna et al (2022) [63] reported that salt and drought treatment significantly induced OsPIN9 expression, indicating the potential role of OsPIN9 in abiotic stress adaptation. In line with this, our previous study showed that mutation of OsPIN9 by CRISPR/Cas9 technology confers enhanced chilling tolerance in rice [64].…”

Section: Introductionmentioning

confidence: 97%

Overexpression of OsPIN9 Impairs Chilling Tolerance via Disturbing ROS Homeostasis in Rice

Ouyang,

Zhang,

Yang

et al. 2023

Plants

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The auxin efflux transporter PIN-FORMED (PIN) family is one of the major protein families that facilitates polar auxin transport in plants. Here, we report that overexpression of OsPIN9 leads to altered plant architecture and chilling tolerance in rice. The expression profile analysis indicated that OsPIN9 was gradually suppressed by chilling stress. The shoot height and adventitious root number of OsPIN9-overexpressing (OE) plants were significantly reduced at the seedling stage. The roots of OE plants were more tolerant to N-1-naphthylphthalamic acid (NPA) treatment than WT plants, indicating the disturbance of auxin homeostasis in OE lines. The chilling tolerance assay showed that the survival rate of OE plants was markedly lower than that of wild-type (WT) plants. Consistently, more dead cells, increased electrolyte leakage, and increased malondialdehyde (MDA) content were observed in OE plants compared to those in WT plants under chilling conditions. Notably, OE plants accumulated more hydrogen peroxide (H2O2) and less superoxide anion radicals (O2−) than WT plants under chilling conditions. In contrast, catalase (CAT) and superoxide dismutase (SOD) activities in OE lines decreased significantly compared to those in WT plants at the early chilling stage, implying that the impaired chilling tolerance of transgenic plants is probably attributed to the sharp induction of H2O2 and the delayed induction of antioxidant enzyme activities at this stage. In addition, several OsRboh genes, which play a crucial role in ROS production under abiotic stress, showed an obvious increase after chilling stress in OE plants compared to that in WT plants, which probably at least in part contributes to the production of ROS under chilling stress in OE plants. Together, our results reveal that OsPIN9 plays a vital role in regulating plant architecture and, more importantly, is involved in regulating rice chilling tolerance by influencing auxin and ROS homeostasis.

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“…In addition to the PIN (PIN-FORMED) family, auxin transport is carried out by other types of proteins: AUX1/LAX (AUXIN-INSENSITIVE1/LIKE AUX1), ABCB (subfamily of ATP-binding cassette transporters), PILS (PIN-LIKES), NRT1.1 (nitrate transporter 1.1), and WAT1 (WALLS ARE THIN1) (Manna et al, 2022). It is believed that at a low pH of the apoplast, auxin becomes protonated and can penetrate into the cell by diffusion.…”

Section: Introductionmentioning

confidence: 99%

Expression of auxin transporter genes in flax (<i>Linum usitatissimum</i>) fibers during gravity response

Ibragimova,

Mokshina

2024

Vestn. VOGiS

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Gravitropism is an adaptive reaction of plants associated with the ability of various plant organs to be located and to grow in a certain direction relative to the gravity vector, while usually the asymmetric distribution of the phytohormone auxin is a necessary condition for the gravitropical bending of plant organs. Earlier, we described significant morphological changes in phloem fibers with a thickened cell wall located on different sides of the stem in the area of the gravitropic curvature. The present study is the first work devoted to the identification of genes encoding auxin transporters in cells at different stages of development and during gravity response. In this study, the flax genes encoding the AUX1/LAX, PIN-FORMED, PIN-LIKES, and ABCB auxin transporters were identified. A comparative analysis of the expression of these genes in flax phloem fibers at different stages of development revealed increased expression of some of these genes at the stage of intrusive growth (LusLAX2 (A, B), LuxPIN1-D, LusPILS7 (C, D)), at the early stage of tertiary cell wall formation (LusAUX1 (A, D), LusABCB1 (A, B), LusABCB15-A, LusPIN1 (A, B), LusPIN4-A, and LusPIN5-A), and at the late stage of tertiary cell wall development (LusLAX3 (A, B)). It was shown that in the course of gravitropism, the expression of many genes, including those responsible for the influx of auxin in cells (LusAUX1-D), in the studied families increased. Differential expression of auxin transporter genes was revealed during gravity response in fibers located on different sides of the stem (upper (PUL) and lower (OPP)). The difference was observed due to the expression of genes, the products of which are responsible for auxin intracellular transport (LusPILS3, LusPILS7-A) and its efflux (LusABCB15-B, LusABCB19-B). It was noted that the increased expression of PIN genes and ABCB genes was more typical of fibers on the opposite side. The results obtained allow us to make an assumption about the presence of differential auxin content in the fibers of different sides of gravistimulated flax plants, which may be determined by an uneven outflow of auxin. This study gives an idea of auxin carriers in flax and lays the foundation for further studies of their functions in the development of phloem fiber and in gravity response.

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Characterization and expression profiling of PIN auxin efflux transporters reveal their role in developmental and abiotic stress conditions in rice

Cited by 9 publications

References 83 publications

Humic acid improves wheat growth by modulating auxin and cytokinin biosynthesis pathways

Humic acid improves wheat growth by modulating auxin and cytokinin biosynthesis pathways

Overexpression of OsPIN9 Impairs Chilling Tolerance via Disturbing ROS Homeostasis in Rice

Expression of auxin transporter genes in flax (<i>Linum usitatissimum</i>) fibers during gravity response

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