Characterization and fine mapping of the rice blast resistance gene Pia

Zeng, Xiaoshan; Yang, Xianfeng; Zhao, Zixiang; Lin, Fei; Wang, Ling; Pan, Qinghua

doi:10.1007/s11427-011-4154-1

Cited by 17 publications

(5 citation statements)

References 30 publications

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“…Pi2 shows robust resistance against 523 isolates of M. oryzae isolated in China (Zhu et al, 2012). Pia shows high resistance to rice blast populations in Jiangsu Province, China (Zeng et al, 2011), and serves as a key gene for breeding rice varieties with other rice blast resistance genes (Wu et al, 2015). Ptr is another broad-spectrum blast resistance gene (Zhao et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Genomic and transcriptomic analyses of the elite rice variety Huizhan provide insight into disease resistance and heat tolerance

Yang,

Yang

et al. 2024

Preprint

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Rice is an important crop and serves as a model for crop genomics and breeding studies. Here, we used Oxford Nanopore ultra-long sequencing and next-generation sequencing technologies to generate a chromosome-scale genome assembly of Huizhan, a disease-resistant and heat-tolerant indica rice variety. The final genome assembly was 395.20 Mb with a scaffold N50 of 31.87 Mb. We identified expanded gene families in Huizhan that are potentially associated with both organ growth and development, as well as stress responses. We observed that three functional rice blast resistance genes, including Pi2, Pia and Ptr, and bacterial blight resistance gene Xa27, likely contribute to disease resistance of Huizhan. In addition, integrated genomics and transcriptomics analyses show that OsHIRP1, OsbZIP60, the SOD gene family, and various transcription factors are likely involved in heat tolerance of Huizhan. Results presented in this study will serve as a valuable resource for rice functional genomics studies and breeding.

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Section: Discussionmentioning

confidence: 99%

Genomic and transcriptomic analyses of the elite rice variety Huizhan provide insight into disease resistance and heat tolerance

Yang,

Yang

et al. 2024

Preprint

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“…93–11. Linkage marker search and prime designation were performed in the way essentially same way as previously described (Liu et al 2005 ; Zeng et al 2011 ; He et al 2012 ). Genomic map of the target locus was constructed on the basis of both reference sequences.…”

Section: Methodsmentioning

confidence: 99%

A New Recessive Gene Conferring Resistance Against Rice Blast

Liang

Wang

Pan

2016

Rice

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BackgroundRice blast (causative pathogen Magnaporthe oryzae) represents a major biotic constraint over rice production. While numerous genes for resistance have been found in both japonica and indica germplasm, as yet the diversity harbored by aus germplasm has not been widely exploited.ResultsThe blast resistance present in the aus type cultivar AS20-1 was shown, via an analysis of segregation in the F2 generation bred from a cross with the highly blast susceptible cultivar Aichi Asahi, to be due to the action of a single recessive gene, denoted pi66(t). The presence of pi66(t) gave an intermediate level control to plants infected with the blast pathogen isolate EHL0635. A bulked segregant analysis indicated that four microsatellite loci (SSRs) mapping to chromosome 3 were probably linked to pi66(t). Localized mapping using chromosome 3-based SSRs and Indels defined a genetic window for pi66(t), flanked by the markers F04-j2 and M19-i12, which physically equals to 27.7 and 49.0 kb, respectively, in the reference genomes of cultivars Nipponbare and 93–11. This physical interval does not harbor any major gene currently associated with disease resistance.Conclusionpi66(t) is one of just three recessive genes controlling rice blast, and is the first major gene for resistance to be mapped to chromosome 3.Electronic supplementary materialThe online version of this article (doi:10.1186/s12284-016-0120-7) contains supplementary material, which is available to authorized users.

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“…25 , Zeng et al . 26 , and Wu et al . 27 independently cloned the blast resistance gene Pia from the loci of NBS R gene TD arrays by genetic mapping of resistant cultivars of O .…”

Section: Introductionmentioning

confidence: 97%

Comparative Genomics Analysis in Grass Species Reveals Two Distinct Evolutionary Strategies Adopted by R Genes

Zhang

Guo

Shen

et al. 2019

Sci Rep

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Resistance genes play an important role in the defense of plants against the invasion of pathogens. In Setaria italica and closely related grass species, R genes have been identified through genetic mapping and genome-wide homologous/domain searching. However, there has been to date no systematic analysis of the evolutionary features of R genes across all sequenced grass genomes. Here, we determined and comprehensively compared R genes in all 12 assembled grass genomes and an outgroup species ( Arabidopsis thaliana ) through synteny and selection analyses of multiple genomes. We found that the two groups of nucleotide binding site (NBS) domains containing R genes—R tandem duplications (TD) and R singletons—adopted different strategies and showed different features in their evolution. Based on K a / K s analysis between syntenic R loci pairs of TDs or singletons, we conclude that R singletons are under stronger purifying selection to be conserved among different grass species than R TDs, while R genes located at TD arrays have evolved much faster through diversifying selection. Furthermore, using the variome datasets of S . italica populations, we scanned for selection signals on genes and observed that a part of R singleton genes have been under purifying selection in populations of S . italica , which is consistent with the pattern observed in syntenic R singletons among different grass species. Additionally, we checked the synteny relationships of reported R genes in grass species and found that the functionally mapped R genes for novel resistance traits are prone to appear in TDs and are heavily divergent from their syntenic orthologs in other grass species, such the black streak R gene Rxo1 in Z . mays and the blast R gene Pi37 in O . sativa . These findings indicate that the R genes from TDs adopted tandem duplications to evolve faster and accumulate more mutations to facilitate functional innovation to cope with variable threats from a fluctuating environment, while R singletons provide a way for R genes to maintain sequence stability and retain conservation of function.

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Characterization and fine mapping of the rice blast resistance gene Pia

Cited by 17 publications

References 30 publications

Genomic and transcriptomic analyses of the elite rice variety Huizhan provide insight into disease resistance and heat tolerance

Genomic and transcriptomic analyses of the elite rice variety Huizhan provide insight into disease resistance and heat tolerance

A New Recessive Gene Conferring Resistance Against Rice Blast

Comparative Genomics Analysis in Grass Species Reveals Two Distinct Evolutionary Strategies Adopted by R Genes

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