1999
DOI: 10.1074/jbc.274.40.28121
|View full text |Cite
|
Sign up to set email alerts
|

Characterization and Function in Vivo of Two Novel Phospholipases B/Lysophospholipases fromSaccharomyces cerevisiae

Abstract: The yeast genome contains two genes, designated as PLB2 and PLB3, that are 67% and 62% identical, respectively, to PLB1, which codes for a phospholipase B/lysophospholipase in yeast (Lee, S. K., Patton, J. L., Fido, M., Hines, L. K., Kohlwein, S. D., Paltauf, F., Henry, S. A., and Levin, D. E. (1994) J. Biol. Chem. 269, 19725-19730). Deletion and overexpression studies and in vivo and in vitro activity measurements suggest that both genes indeed code for phospholipases B/lysophospholipases. In cell free extrac… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1
1
1

Citation Types

9
118
0
1

Year Published

2001
2001
2019
2019

Publication Types

Select...
6
1

Relationship

0
7

Authors

Journals

citations
Cited by 102 publications
(128 citation statements)
references
References 35 publications
9
118
0
1
Order By: Relevance
“…Therefore, the rapid hydrolysis, which involves the action of different lysophospholipases secreted from the aleurone layers Baisted, 1984, 1986;Fujikura and Baisted, 1985), would release quantitative amounts of GroPCho in the endosperm. In addition to germination, we should mention four hypothetical situations that could trigger glycerophosphodiester accumulation in the extracellular compartment: (a) Although there is no evidence of excretion of glycerophosphodiesters in plants, this mechanism is widespread in other eukaryotes like yeast (Angus and Lester, 1975;Dowd et al, 2001) and animal cells (Barburina and Jackowski, 1999); (b) the phospholipid turnover of the plasma membrane may also lead to the production of extracellular glycerophosphodiesters because in yeast, it implies the action of extracellular acyltransferase and phospholipase B (Merkel et al, 1999); van der Rest et al (c) transport of GroPCho through the xylem may occur because Martin and Tolbert (1983) measured high concentrations of P-Cho in the xylem sap; and (d) in physiological situations that affect the plant integrity (wounding, pathogen attack, and senescence), intracellular pools of organic-phosphate are released in the extracellular medium whereas actions of different lipases release phospholipids catabolites. The different phosphatases and phosphodiesterase may allow reuse of various catabolites in the neighborhood of the damaged tissues.…”
Section: Physiological Significance Of Plant Gpc-pde Activitymentioning
confidence: 99%
“…Therefore, the rapid hydrolysis, which involves the action of different lysophospholipases secreted from the aleurone layers Baisted, 1984, 1986;Fujikura and Baisted, 1985), would release quantitative amounts of GroPCho in the endosperm. In addition to germination, we should mention four hypothetical situations that could trigger glycerophosphodiester accumulation in the extracellular compartment: (a) Although there is no evidence of excretion of glycerophosphodiesters in plants, this mechanism is widespread in other eukaryotes like yeast (Angus and Lester, 1975;Dowd et al, 2001) and animal cells (Barburina and Jackowski, 1999); (b) the phospholipid turnover of the plasma membrane may also lead to the production of extracellular glycerophosphodiesters because in yeast, it implies the action of extracellular acyltransferase and phospholipase B (Merkel et al, 1999); van der Rest et al (c) transport of GroPCho through the xylem may occur because Martin and Tolbert (1983) measured high concentrations of P-Cho in the xylem sap; and (d) in physiological situations that affect the plant integrity (wounding, pathogen attack, and senescence), intracellular pools of organic-phosphate are released in the extracellular medium whereas actions of different lipases release phospholipids catabolites. The different phosphatases and phosphodiesterase may allow reuse of various catabolites in the neighborhood of the damaged tissues.…”
Section: Physiological Significance Of Plant Gpc-pde Activitymentioning
confidence: 99%
“…Turbidity was monitored by measuring the optical density at 600 nm (A 600 ) on a BioMate 3 Thermo Spectronic spectrophotometer. Empty vectors, YEp351 and pRS426, and vectors overexpressing each of the PLB genes, YEp351-PLB1, YEp351-PLB2, YEp351-PLB3, and pRS426-NTE1, were gifts from Susan Henry (YEp351 series) (21) and Chris McMaster (pRS426-NTE1) (31). Media used for this study included rich yeast extract peptone dextrose (YPD) medium purchased from Fisher or synthetic complete yeast nitrogen base (YNB) medium containing amino acids, 2% glucose, and 75 M inositol, as described previously (32).…”
Section: Methodsmentioning
confidence: 99%
“…The Secreted and Cell-associated Forms of Plb1 Increase in ypk1⌬ Strain-Plb1 activity has been reported in plasma membrane fractions, in the periplasm, and in the culture supernatant (19,21). However, localization data were absent in large scale analyses using fluorescent tags fused to the C termini of yeast ORFs expressed under control of their endogenous promoters (56 -60) and in a study utilizing C-terminal fusions to an epitope tag with the target genes expressed under control of the GAL promoter (61).…”
Section: The Rate Of Short Term Incorporation Of [ 14 C]choline Into mentioning
confidence: 99%
See 2 more Smart Citations