Characterization and functional analysis of <i>hsp18.3</i> gene in the red flour beetle, <i>Tribolium castaneum</i>

Xie, Jia; Hu, Xingxing; Zhai, Mengfan; Yu, Xiaojuan; Song, Xiaodong; Gao, Shanshan; Wu, Wei

doi:10.1111/1744-7917.12543

Cited by 30 publications

(27 citation statements)

References 53 publications

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“…As T. castaneum is basically living in the warm cereal storage place and without diapause, Tchsp21.8b was in response to heat but exhibited insensitiveness to cold stress. This phenotype was also existed in ER‐based Tchsp90 and Tchsp18.3 , both of which were only upregulated by heat stress (Xie et al., ; Zhang, Gu et al., ), indicating that the behaviour would make a similar induction of other hsps in T. castaneum .…”

Section: Discussionmentioning

confidence: 92%

“…Particularly, although some of the orthologous shsps could be upregulated or inhibited under the different stresses, their responses are negligible compared to the species‐specific shsps and the members of hsp70 and hsp90 family. Suffering the extreme temperature stimulation, many species‐specific shsps can be induced hundreds or even thousands folds, such as Tchsp18.3 , Pxhsp20.09 , Cshsp21.7b and so on (Chen & Zhang, ; Lu et al., ; Xie et al., ). Likewise, Tchsp18.3 , Tchsp68 and Tchsp83 mRNA levels significantly increased during the first 2 hr of UV exposure and the highest upregulation could reach to 8.90‐fold (Sang et al., ).…”

Section: Discussionmentioning

confidence: 99%

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Characterization and functional analysis of hsp21.8b: An orthologous small heat shock protein gene in Tribolium castaneum

Xie

Xiong

et al. 2018

J Applied Entomology

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Small heat shock proteins (sHSPs) act as molecular chaperones and are widely distributed in all kinds of organisms. Comparative analysis revealed that an orthologous shsp was present during insect evolution. Here, hsp21.8b, one insect orthologous shsp, had been identified in Tribolium castaneum. Quantitative real‐time PCR illustrated that Tchsp21.8b was expressed in all developmental stages, along with the lowest expression at early embryonic stage and relative high expression at other stages especially in late eggs and late pupae. In the adult period, Tchsp21.8b exhibited the highest expression level in central nervous system and followed in elytron, epidermis, ovary and fat body. Moreover, it was upregulated 3.39‐fold in response to enhanced heat stress (45°C) for 4 hr but not to cold stress (4°C) and was upregulated by 1.73‐ to 1.94‐fold under ultraviolet (UV) exposure during 4–6 hr. It was also downregulated by 20.8%–41.8% under starvation in 3 days and had a “down‐up‐down” trend under the pathogen stresses. Larval RNA interference of Tchsp21.8b caused 40.6% insects mortality and reduced the oviposition amount by 66.0% and only 21.0% of the ds‐Tchsp21.8b eggs could hatch into larvae. These results suggested that as an orthologous shsp, Tchsp21.8b not only plays important roles in the growth, development and fecundity of T. castaneum but with the competence to resist the environment stresses, although the response is relatively weak compared to other hsps. Results from this study also uncovered the functions of the orthologous shsp in the development and anti‐stresses ability of T. castanuem. It provided more scientific evidence for revealing the physiological mechanisms of shsps of the insects and enhanced the capabilities to control different pests.

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Section: Discussionmentioning

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Characterization and functional analysis of hsp21.8b: An orthologous small heat shock protein gene in Tribolium castaneum

Xie

Xiong

et al. 2018

J Applied Entomology

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“…Previous studies have demonstrated that heat shock protein genes affect insect reproduction and longevity. Xie et al (2019) studied the functional of hsp18.3 gene in the Tribolium castaneum and found that after injection of dshsp18.3, one pair of the beetles can hardly spawn. When Tchsp18.3 was knocked down, the survival rate of adults and fecundity were significantly lower than the control group.…”

Section: Discussionmentioning

confidence: 99%

Induced Thermotolerance and Expression of Three Key Hsp Genes (Hsp70, Hsp21, and sHsp21) and Their Roles in the High Temperature Tolerance of Agasicles hygrophila

et al. 2020

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Thermal adaptation plays a fundamental role in the expansion and distribution of insects, and heat shock proteins (Hsps) play important roles in the temperature adaptation of various organisms. To determine the roles of Hsp genes (Hsp70, Hsp21, and sHsp21) on the high temperature tolerance of Agasicles hygrophila, we obtained complete cDNA (complementary DNA) sequences for Hsp70, Hsp21, and sHsp21 by rapid amplification of cDNA ends (RACE), analyzed their expression profiles under different high temperature treatments by quantitative reverse transcription polymerase chain reaction (RT-qPCR), and performed functional verification by RNA interference (RNAi). The open reading frames of Hsp70, Hsp21, and sHsp21 were 1940, 543, and 567 bp, encoding 650, 180, and 188 amino acids, respectively. Their molecular weights (MWs) were 71.757, 20.879, and 21.510 kDa, and the isoelectric points were 5.63, 6.45, and 6.24, respectively. Phylogenetic tree analysis showed that the Hsp70, Hsp21, and sHsp21 genes of A. hygrophila were relatively conserved in evolution. The Hsp70 and Hsp21 genes in A. hygrophila were homologous to those in Leptinotarsa decemlineata (87 and 79% similarity, respectively), and the sHsp21 gene in A. hygrophila was homologous to that in Lissorhoptrus oryzophilus (74% similarity). The amino acid polypeptide chain had highly conserved sequences of DLGGGTFD, VLVGGSTR, and GPTIEEVD. The sequence of EEVD was the characteristic motif of cytoplasmic Hsp70, and the highly conserved sequences of MALFR and MSLLP were characteristic sequences of Hsp2 and sHsp21, respectively. Relative quantitative real time PCR showed that the three Hsps could be induced by 4-h treatment at high temperatures. Significant upregulation of these Hsps was observed when the temperature was further increased. The RNAi results showed that the injection of the three Hsps' dsRNA could suppress the expression at the gene level significantly. Compared with the control group,

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“…Then, a total of 200 ng of the dsRNA in 200 nL solution was injected into the body cavity of each larva (∼15 days old). Injection of an equal volume of physiological buffer only (IB) and non‐injection (wild‐type, WT) were served as negative controls . Three biological replications with independent injections were conducted.…”

Section: Methodsmentioning

confidence: 99%

“…Injection of an equal volume of physiological buffer only (IB) and non-injection (wild-type, WT) were served as negative controls. 46,54 Three biological replications with independent injections were conducted. Additionally, to confirm that the results we observed in the subsequent RNAi analysis were due to suppression of the expression levels of the specific target genes and not to the presence of dsRNAs, we also injected GFP dsRNA in an equal volume of IB into 15-day-old T. castaneum larvae.…”

Section: Double-stranded Rna Synthesis and Injectionmentioning

confidence: 99%

CYP4BN6 and CYP6BQ11 mediate insecticide susceptibility and their expression is regulated by Latrophilin in Tribolium castaneum

Xiong

Gao

Mao

et al. 2019

Pest Management Science

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BACKGROUND Many insect cytochrome P450 proteins (CYPs) are involved in the metabolic detoxification of exogenous compounds such as plant toxins and insecticides. Tribolium castaneum, the red flour beetle, is a major agricultural pest that damages stored grains and cereal products. With the completion of the sequencing of its genome, two T. castaneum species‐specific CYP genes, CYP4BN6, and CYP6BQ11, were identified. However, it is unknown whether the functions of most CYPs are shared by TcCYP4BN6 and TcCYP6BQ11, and the upstream regulatory mechanism of these two CYPs remains elusive. RESULTS QRT‐PCR analysis indicated that TcCYP4BN6 and TcCYP6BQ11 were both most highly expressed at the late pupal stage and were mainly observed in the head and gut, respectively, of adults. Moreover, the transcripts of these two CYPs were significantly induced by dichlorvos and carbofuran, and RNA interference (RNAi) targeting of each of them enhanced the susceptibility of beetles to these two insecticides. Intriguingly, knockdown of the latrophilin (lph) gene, which has been reported to be related to the insecticide susceptibility, reduced the expression of TcCYP4BN6 and TcCYP6BQ11 after insecticide treatment, suggesting that these two CYP genes are regulated by lph to participate in insecticide susceptibility in T. castaneum. CONCLUSION These results shed new light on the function and mechanism of CYP genes associated with insecticide susceptibility and could facilitate research on appropriate and sustainable pest control management. © 2019 Society of Chemical Industry

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Characterization and functional analysis of hsp18.3 gene in the red flour beetle, Tribolium castaneum

Cited by 30 publications

References 53 publications

Characterization and functional analysis of hsp21.8b: An orthologous small heat shock protein gene in Tribolium castaneum

Characterization and functional analysis of hsp21.8b: An orthologous small heat shock protein gene in Tribolium castaneum

Induced Thermotolerance and Expression of Three Key Hsp Genes (Hsp70, Hsp21, and sHsp21) and Their Roles in the High Temperature Tolerance of Agasicles hygrophila

CYP4BN6 and CYP6BQ11 mediate insecticide susceptibility and their expression is regulated by Latrophilin in Tribolium castaneum

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