Characterization and functional analysis of a novel PCP gene BcMF5 from Chinese cabbage (Brassica campestris L. ssp. chinensis Makino)

Qiang, Zhang; Cao, Jiashu; Liu, Huizhi; Huang, Li; Xiao, Xiang; Yu, Xiaolin

doi:10.1016/j.jplph.2007.06.020

Cited by 10 publications

(3 citation statements)

References 25 publications

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“…The construct did, however, show small differences in the level of target gene expression among various transgenic plants. This result was consistent with that of previous studies showing normal CaMV 35S promoter activity in the pollen and anthers of Chinese cabbage (Cao et al , 2006; Li et al , 2007; Zhang et al , 2008). Therefore, it is feasible to use the CaMV 35S promoter as a substitute when the gene's own promoter is not available in investigations of Chinese cabbage pollen or anther genes.…”

Section: Discussionsupporting

confidence: 94%

The polygalacturonase gene BcMF2 from Brassica campestris is associated with intine development

Huang

Cao

Zhang

et al. 2008

Journal of Experimental Botany

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Brassica campestris Male Fertility 2 (BcMF2) is a putative polygalacturonase (PG) gene previously isolated from the flower bud of Chinese cabbage (Brassica campestris L. ssp. chinensis Makino, syn. B. rapa ssp. chinensis). This gene was found to be expressed specifically in tapetum and pollen after the tetrad stage of anther development. Antisense RNA technology was used to study the function of BcMF2 in Chinese cabbage. Scanning and transmission electron microscopy revealed that there were deformities in the transgenic mature pollen grains such as abnormal location of germinal furrows. In addition, the homogeneous pectic exintine layer facing the exterior seemed to be overdeveloped and predominantly occupied the intine, thus reversing the normal proportional distribution of the internal endintine layer and the external exintine layer. Since it is a continuation of the intine layer, the pollen tube wall could not grow normally. This resulted in the formation of a balloon-like swelling structure in the pollen tube tip in nearly 80% of the transgenic pollen grains. Premature degradation of tapetum was also found in these transgenic plants, which displayed decreased expression of the BcMF2 gene. BcMF2 might therefore encode a new PG with an important role in pollen wall development, possibly via regulation of pectin's dynamic metabolism.

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Section: Discussionsupporting

confidence: 94%

The polygalacturonase gene BcMF2 from Brassica campestris is associated with intine development

Huang

Cao

Zhang

et al. 2008

Journal of Experimental Botany

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“…The study of anther development is important for Chinese cabbage breeding. Though several genes related to anther development have been identified in Chinese cabbage, such as BAN103 3 , BcMS2 4 , BcMF2 5 , BcMF5 6 , BcMF6 7 , BrSKS13 8 , BcMF12 9 , BcMF13 10 , BcMF19 11 , and BcMF20 12 , the molecular mechanism of anther development remains unstudied.…”

Section: Introductionmentioning

confidence: 99%

Whole-transcriptome analysis and construction of an anther development-related ceRNA network in Chinese cabbage (Brassica campestris L. ssp. pekinensis)

Shi

Pang

Liu

et al. 2022

Sci Rep

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Anther development is precisely regulated by a complex gene network, which is of great significance to plant breeding. However, the molecular mechanism of anther development in Chinese cabbage is unclear. Here, we identified microRNAs (miRNAs), mRNAs, long non-coding RNAs (lncRNAs), and circular RNAs (circRNAs) related to anther development in Chinese cabbage (Brassica campestris L. ssp. pekinensis) to construct competitive endogenous RNA (ceRNA) regulatory networks and provide valuable knowledge on anther development. Using whole-transcriptome sequencing, 9055, 585, 1344, and 165 differentially expressed mRNAs (DEmRNAs), miRNAs (DEmiRNAs), lncRNAs (DElncRNAs), and circRNAs (DEcircRNAs) were identified, respectively, in the anthers of Chinese cabbage compared with those in samples of the vegetative mass of four true leaves. An anther-related ceRNA regulatory network was constructed using miRNA targeting relationships, and 450 pairs of ceRNA relationships, including 97 DEmiRNA–DEmRNA, 281 DEmiRNA–DElncRNA, and 23 DEmiRNA–DEcircRNA interactions, were obtained. We identified important genes and their interactions with lncRNAs, circRNAs, and miRNAs involved in microsporogenesis, tapetum and callose layer development, pollen wall formation, and anther dehiscence. We analyzed the promoter activity of six predominant anther expression genes, which were expressed specifically in the anthers of Arabidopsis thaliana, indicating that they may play an important role in anther development of Chinese cabbage. This study lays the foundation for further research on the molecular mechanisms of anther growth and development in Chinese cabbage.

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“…Taking male sterility as a starting point, several studies of pollen development genes in pak choi have been conducted since 2001 (Han, 2011). A series of genes related to pollen development have been isolated, including CYP86MF (Yu et al, 2004), BcMF1 (Wang, 2003), BcMF2 (Wang et al, 2005), BcMF3 and BcMF4 (Liu et al, 2007), BcMF5 and BcMF6 (Zhang et al, 2008), BcMF7 (Huang et al, 2007), BcMF8 (Huang et al, 2008a), BcMF9 (Huang et al, 2009), BcMF10 (Huang et al, 2008b), BcMF11 (Song et al, 2007), BcMF12, BcMF13 (Li et al, 2008), BcMF19 (Huang et al, 2011), BcMF22 (Li, 2011), BcMFJMJ30, BcBGAL11, andMIR158 (Jiang, 2014), BcMF20 (Han, 2011), BcAHA8 (Qiu, 2012) and BcSKS11 (Zhang, 2011), BcMYB, and BjMF6. The functions of some of these genes have been described, including the cytochrome P450 gene CYP86MF, the pectinesterase gene BcMF3, the polygalacturonase genes BcMF2, BcMF6, and BcMF9, the arabinogalactan protein genes BcMF8 and BcMF18, the beta-galactosidase gene BcBGAL11, and the pollen wall protein gene BcMF5.…”

Section: Introductionmentioning

confidence: 99%

Identification of genes related to floral organ development in pak choi by expression profiling

et al. 2017

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ABSTRACT. Pak choi is a highly nutritious vegetable that is widely grown in China, Southeast Asia, and other parts of the world. Because it reproduces by seed, it is very important to understand the mechanism of floral organ development. Therefore, using the Chinese cabbage genome as a reference, this study analyzed the expression profiles of shoot apex genes at flower bud differentiation stages 1 and 5, in order to identify genes related to floral organ development. The results showed that the proportion of mapped genes was high, with 84.25 and 83.80% of clean reads from the two sample saligned to the reference genome, respectively. A total of 525 differentially expressed genes (DEGs) were identified, 224 of which were upregulated and 301 were downregulated. The expression levels of genes homologous to Chinese cabbage flowering genes were also analyzed at stages 1 and 5; the expression 2 H.X. Song et al.Genetics and Molecular Research 16 (1): gmr16019233 levels of Bra012997 (ap1), Bra000393 (SOC1), and Bra004928 (SOC1) were significantly upregulated at stage 5, suggesting that these three genes positively regulate floral development in pak choi. DEGs involved in floral organ development were analyzed with homologous genes from Arabidopsis thaliana; the homologous genes Bra029281 (AGL42), Bra026577 (ARPN), Bra022954 (SPL3), Bra029293 (ARF2), Bra007978 (AtRLP12), Bra033221 (SPL8), Bra008037 (LOX4), Bra001598 (IAA19), Bra003892 (PATL1), Bra038778 (AT4G21323), Bra025315 (KLCR2), and Bra013906 (DTX35) are directly related to floral organ development in Arabidopsis, suggesting that these genes have corresponding functions during flower organ development in pak choi, and could be candidates for further genetic research. These results provide a foundation for research on the molecular mechanism of flower organ development in pak choi and other Brassica rapa vegetables.

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Characterization and functional analysis of a novel PCP gene BcMF5 from Chinese cabbage (Brassica campestris L. ssp. chinensis Makino)

Cited by 10 publications

References 25 publications

The polygalacturonase gene BcMF2 from Brassica campestris is associated with intine development

The polygalacturonase gene BcMF2 from Brassica campestris is associated with intine development

Whole-transcriptome analysis and construction of an anther development-related ceRNA network in Chinese cabbage (Brassica campestris L. ssp. pekinensis)

Identification of genes related to floral organ development in pak choi by expression profiling

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