Characterization and functional analysis of a MADS-box transcription factor gene (GbMADS9) from Ginkgo biloba

Yang, Fen; Xu, Feng; Wang, Xiaohui; Liao, Yongling; Chen, Qiangwen; Meng, Xiangxiang

doi:10.1016/j.scienta.2016.09.042

Cited by 33 publications

(26 citation statements)

References 79 publications

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“…GbMADS9 is a Ginkgo biloba MADS-box gene involved in post-germination root growth in response to abiotic stress tolerance (Yang et al, 2016); it is an ortholog of the B sister-class genes of Arabidopsis. GbMADS9 is upregulated in response to salt, drought and cold stresses (Figure 3); and Arabidopsis OE lines of this gene exhibited longer roots than those of wild type plants after 15 days under high osmotic stress (400 mM of mannitol).…”

Section: Mads-domain Transcription Factors' Role In Developmental mentioning

confidence: 99%

“…Proline is an amino acid that may enhance stress tolerance because it works as an osmolyte, a metal chelator, an antioxidative defense molecule, and a signaling molecule (Hayat et al, 2012). Finally, enhanced tolerance to osmotic conditions was associated with improved superoxide dismutase and catalase antioxidant enzymatic activities in these OE lines (Yang et al, 2016). These data suggest that the increase tolerance of the OE lines of GbMADS9 , could be dependent on the proline content and the enhance of antioxidant activities.…”

Section: Mads-domain Transcription Factors' Role In Developmental mentioning

confidence: 99%

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MADS-Box Genes Are Key Components of Genetic Regulatory Networks Involved in Abiotic Stress and Plastic Developmental Responses in Plants

et al. 2019

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Plants, as sessile organisms, adapt to different stressful conditions, such as drought, salinity, extreme temperatures, and nutrient deficiency, via plastic developmental and growth responses. Depending on the intensity and the developmental phase in which it is imposed, a stress condition may lead to a broad range of responses at the morphological, physiological, biochemical, and molecular levels. Transcription factors are key components of regulatory networks that integrate environmental cues and concert responses at the cellular level, including those that imply a stressful condition. Despite the fact that several studies have started to identify various members of the MADS-box gene family as important molecular components involved in different types of stress responses, we still lack an integrated view of their role in these processes. In this review, we analyze the function and regulation of MADS-box gene family members in response to drought, salt, cold, heat, and oxidative stress conditions in different developmental processes of several plants. In addition, we suggest that MADS-box genes are key components of gene regulatory networks involved in plant responses to stress and plant developmental plasticity in response to seasonal changes in environmental conditions.

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Section: Mads-domain Transcription Factors' Role In Developmental mentioning

confidence: 99%

Section: Mads-domain Transcription Factors' Role In Developmental mentioning

confidence: 99%

MADS-Box Genes Are Key Components of Genetic Regulatory Networks Involved in Abiotic Stress and Plastic Developmental Responses in Plants

et al. 2019

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“…The annotated GIGANTEA activates TFs encoded by the zinc finger gene CONSTANS , which may mediate the circadian clock and control of flowering 78 . Moreover, the encoded B-sister MADS-box TFs may also have a positive function in ovule development 79 . The female cone utilizes a variety of mechanisms to ensure the normal progress of pollination during development, including the induction of various biochemical and physiological reactions to perfect itself.…”

Section: Discussionmentioning

confidence: 99%

De novo assembly of the Platycladus orientalis (L.) Franco transcriptome provides insight into the development and pollination mechanism of female cone based on RNA-Seq data

Zhou

Chen

Wang

et al. 2019

Sci Rep

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For seed-bearing plants, the basis of seed and fruit formation is pollination. The normal progression of pollination is through advances in continuous signal exchange and material transfer, which occur mainly in female reproductive organs; thus, the molecular mechanism of development in female reproductive organs is vital for understanding the principle of pollination. However, molecular biology studies on the development of female cones related to pollination are rare and unclear in gymnosperms, especially in Cupressaceae. In this study, Platycladus orientalis , a monotypic genus within Cupressaceae, was chosen to examine female cone transcriptomes at pre-pollination and pollination stages by Illumina paired-end sequencing technology to de novo sequence six libraries with 3 biological replicates. These libraries were used to construct a P . orientalis transcriptome database containing 71,669 unigenes (4,963 upregulated unigenes and 11,747 downregulated unigenes at the pollination stage) for subsequent analysis. Based on the annotations and expression levels, the functions of differentially expressed unigenes and enriched pathways between the developmental processes of female cones were analysed to detail the preliminary development and pollination mechanism of the female cone. Targeted investigations were specifically performed to determine the elementary mechanism of secretion and functioning of the pollination drop, a vital ovule secretion at the pollination stage. Ultimately, the expression of 15 unigenes selected between two stages were further assessed and confirmed using qRT-PCR, which demonstrated reliable data and significant differences in the expression profiles of key genes. As one of the largest available transcriptomic resources of this species, the database is constructed to prospectively adapt to the physiological and genomic data of woody plants. This work provided the first transcriptome profile of P . orientalis female cones at different developmental stages, and will promote the illumination of the pollination mechanism of P . orientalis , and will serve as the basis for in-depth genomic study in the Cupressaceae family. This initiative will arouse the interest and attention of scholars and pave the way for future studies.

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“…Few studies provided information about genetic mechanism of flowering in G. biloba . Although our previous work cloned and function reported some genes involved in flower development including three MADS-box transcription factor genes [ 26 – 28 ] and two circadian-regulated genes ( GbCO and GbFT ) in photoperiodic pathway [ 4 , 29 ]. However, little literature reported genetic regulation of sexual differentiation and sexual determination of G. biloba , especially for miRNA identification of strobili sexual differentiation and its regulatory mechanism.…”

Section: Introductionmentioning

confidence: 99%

Screening and identification of miRNAs related to sexual differentiation of strobili in Ginkgo biloba by integration analysis of small RNA, RNA, and degradome sequencing

Liu

Cheng

et al. 2020

BMC Plant Biol

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Background: Ginkgo biloba, a typical dioecious plant, is a traditional medicinal plant widely planted. However, it has a long juvenile period, which severely affected the breeding and cultivation of superior ginkgo varieties. Results: In order to clarify the complex mechanism of sexual differentiation in G. biloba strobili. Here, a total of 3293 miRNAs were identified in buds and strobili of G. biloba, including 1085 known miRNAs and 2208 novel miRNAs using the three sequencing approaches of transcriptome, small RNA, and degradome. Comparative transcriptome analysis screened 4346 and 7087 differentially expressed genes (DEGs) in male buds (MB) _vs_ female buds (FB) and microstrobilus (MS) _vs_ ovulate strobilus (OS), respectively. A total of 6032 target genes were predicted for differentially expressed miRNA. The combined analysis of both small RNA and transcriptome datasets identified 51 miRNA-mRNA interaction pairs that may be involved in the process of G. biloba strobili sexual differentiation, of which 15 pairs were verified in the analysis of degradome sequencing. Conclusions: The comprehensive analysis of the small RNA, RNA and degradome sequencing data in this study provided candidate genes and clarified the regulatory mechanism of sexual differentiation of G. biloba strobili from multiple perspectives.

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Characterization and functional analysis of a MADS-box transcription factor gene (GbMADS9) from Ginkgo biloba

Cited by 33 publications

References 79 publications

MADS-Box Genes Are Key Components of Genetic Regulatory Networks Involved in Abiotic Stress and Plastic Developmental Responses in Plants

MADS-Box Genes Are Key Components of Genetic Regulatory Networks Involved in Abiotic Stress and Plastic Developmental Responses in Plants

De novo assembly of the Platycladus orientalis (L.) Franco transcriptome provides insight into the development and pollination mechanism of female cone based on RNA-Seq data

Screening and identification of miRNAs related to sexual differentiation of strobili in Ginkgo biloba by integration analysis of small RNA, RNA, and degradome sequencing

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