Characterization and functional recovery of a novel antimicrobial peptide (CECdir–CECret) from inclusion bodies after expression in Escherichia coli

Novel Expression Vector for Secretion of Cecropin AD in Bacillus subtilis with Enhanced Antimicrobial Activity

“…5 and 6) confirmed the formation of an amphipathic ␣-helix by N-terminal amino acids 1 through 11 of cecropin A, which is thought to be critical for the activity of cecropins (8). From the results of the antibacterial assays, for some bacteria recombinant cecropin AD showed more effective bactericidal activity than those of cecropins A, B, and D (18) and some other cecropins that have been reported (21,34,48).…”

“…In order to facilitate the expression of cecropin AD, this system included the SUMO protease gene, which could be released under appropriate conditions, thus ensuring the cleavage of the target gene (4). In addition, the modified expression system required no inducer during the process of expression (21), and it produced no inclusion bodies (34,47). Therefore, the system had the advantage of a high efficiency of expression at a low cost and was easy to operate, making it applicable to industrial production.…”

“…However, the expression of foreign proteins in E. coli often leads to the formation of densely packed denatured peptide molecules in the form of insoluble particles called inclusion bodies. Inclusion body proteins are devoid of biological activity, and an elaborate process involving solubilization, refolding, and purification is needed to partially recover a functionally active product (34). While use of a yeast expression system such as P. pastoris may solve the problem of posttranslational modifications (21), it requires a significant investment during the later period of batch cultivation, which renders it uneconomical.…”

Synthetic hepcidin from fish: Uptake and protection against Vibrio anguillarum in sea bass (Dicentrarchus labrax)

“…Antibacterial activity was determined using the microplate assay as previously described [25][26][27]. Serial dilutions of synthetic peptides, beginning at 100 µM were mixed with 100 µL of an exponential phase bacterial culture of V. anguillarum (OD 0.3-0.4).…”

An immunological method for quantifying antibacterial activity in Salmo salar (Linnaeus, 1758) skin mucus

“…Normally this is evaluated using a bacterial growth inhibition test [26,27]. The aim of this work was to establish a correlation between antibacterial activity and immunological quantification of SAMP H1 from Salmo salar skin mucus.…”