Characterization and genetic distance analysis of isolates of Peronosclerospora sorghi using AFLP fingerprinting

Perumal, Ramasamy; Isakeit, Thomas; Menz, Monica A.; Katile, Seriba; No, Eun-Gyu; Magill, Clint

doi:10.1016/j.mycres.2005.12.007

Cited by 19 publications

(16 citation statements)

References 25 publications

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“…b Lane 1, DNA size marker; Lanes 2-5, P. sorghi isolates from sorghum (S4-S7); Lanes 6-8, P. sorghi isolates from maize (M4-M6). Arrow indicates the 1.0 kb fragment of P. sorghi to design SCAR markers Mathiyazhagan et al 2006;Perumal et al 2006). A rapid, accurate, and sensitive method for differentiation of maize and sorghum isolates is essential to disease management and is a prerequisite for studies on environmental and cultural factors related to disease occurrence.…”

Section: Resultsmentioning

confidence: 99%

“…Isakeit and Jaster (2005) reported the existence of a new pathotype of P. sorghi in Texas which showed metalaxyl resistance. Perumal et al (2006) analyzed the genetic variability among the 14 isolates of P. sorghi including metalaxyl resistant and susceptible as well as common pathotypes 1 and 3 and reported that approximately 25% of the bands were polymorphic across the isolates tested with the majority of differences specific to the pathotype P1. Mathiyazhagan et al (2006) reported the genetic variability between the isolates from sorghum and corn using restriction fragment length polymorphism (RFLP) analysis of the polymerase chain reaction (PCR)-amplified internal transcribed spacer (ITS) region of ribosomal DNA.…”

Section: Introductionmentioning

confidence: 99%

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Molecular identification of isolates of Peronosclerospora sorghi from maize using PCR-based SCAR marker

Ladhalakshmi

Vijayasamundeeswari

Paranidharan

et al. 2009

World J Microbiol Biotechnol

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The fungus Peronosclerospora sorghi [Weston and Uppal (Shaw)] infects both sorghum and maize and incites downy mildew disease. Pathogenic and molecular variability among isolates of P. sorghi from sorghum and maize has been reported. In the present study we developed a DNA sequence characterized amplified region (SCAR) marker for identification of isolates of P. sorghi from maize by using polymerase chain reaction (PCR). The random amplified polymorphic DNA (RAPD) primer OPB15 consistently amplified a 1,000 base pairs (bp) product in PCR only from DNA of P. sorghi isolates from maize and not from isolates of sorghum. The PCR-amplified 1,000-bp product was cloned and sequenced. The sequence of the SCAR marker was used for designing specific primers for identification of maize isolates of P. sorghi. The SCAR primers amplified a 800 bp fragment only from genomic DNA of maize isolates of P. sorghi. The SCAR primers developed in this study are highly specific and reproducible, and proved to be powerful tool for identification of P. sorghi isolates from maize.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Molecular identification of isolates of Peronosclerospora sorghi from maize using PCR-based SCAR marker

Ladhalakshmi

Vijayasamundeeswari

Paranidharan

et al. 2009

World J Microbiol Biotechnol

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show abstract

“…Isakeit and Jaster ( 2005 ) reported the existence of a new pathotype of P. sorghi in Texas which showed metalaxyl resistance. Perumal et al ( 2006 ) analyzed the genetic variability among the 14 isolates of P. sorghi including metalaxyl resistant and susceptible as well as common pathotypes 1 and 3 and reported that approximately 25 % of the bands were polymorphic across the isolates tested with the majority of differences specifi c to the pathotype P1. Mathiyazhagan et al ( 2006 ) reported the genetic variability between the isolates from sorghum and corn using restriction fragment length polymorphism (RFLP) analysis of the polymerase chain reaction (PCR)-amplifi ed internal transcribed spacer (ITS) region of ribosomal DNA (Fig.…”

Section: Downy Mildewmentioning

confidence: 99%

Sustainable Agriculture Reviews

2015

Sustainable Agriculture Reviews

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“…A análise das populações utilizando um AFLP modificado permitiu a caracterização e diferenciação de isolados resistentes ao produto. De acordo com os autores, a população deve representar um novo patotipo de P. sorghi (Perumal et al, 2006). Para garantir a vida util do metalaxil, deve-se combiná-lo com outros fungicidas e com outras estratégias de manejo da doença, inclusive o uso de práticas culturais e cultivares resistentes, que reduzam a população do patógeno (Jeger et al, 1998).…”

Section: Controle Químicounclassified

Peronosclerospora sorghi, o agente etiológico do míldio do sorgo

Barbosa

Pfenning

Casela³

2006

Fitopatol. bras.

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RESUMOO agente etiológico do míldio do sorgo, Peronosclerospora sorghi, infecta as culturas do sorgo (Sorghum spp.) e do milho (Zea mays). Esse patógeno encontra-se disseminado em muitas regiões tropicais e subtropicais do mundo e pode ocasionar danos significativos na produção de sorgo quando as condições climáticas são favoráveis à sua ocorrência e em cultivares de alta susceptibilidade. No Brasil, antes restrito aos estados da região Sul, o míldio foi registrado também nos estados da região Sudeste e Centro-Oeste, causando prejuízos principalmente em áreas de produção de sementes. O cultivo de genótipos resistentes é o método mais eficiente para o controle da doença. Entretanto, essa estratégia é dificultada pela alta variabilidade genética apresentada pelo patógeno. Essa revisão aborda aspectos da taxonomia, biologia e distribuição geográfica do míldio do sorgo e discute questões relacionadas com a sua epidemiologia e controle, enfatizando estratégias que utilizam resistência genética.Palavras-chave adicionais: Straminipila, Sclerosporales, oomiceto, fitodoença, epidemiologia, controle, resistência. ABSTRACT Peronosclerospora sorghi, the causal agent of sorghum downy mildewThe causal agent of sorghum downy mildew, Peronosclerospora sorghi, is a pathogen of sorghum species (Sorghum spp.) and corn (Zea mays). The pathogen is disseminated in many tropical and subtropical regions all around the world, causing considerable losses when conditions are favorable for its development or susceptible cultivars are used. Within Brazil it was initially restricted to the Southern region but has now also spread to the Southeast and Middle-West, causing significant losses specifically in areas of seed production. The use of resistant genotypes is the most efficient method to control the disease. Nevertheless, a high genetic variability of the pathogen makes it difficult to use resistant cultivars. This revision considers aspects of taxonomy and biology of sorghum downy mildew and discusses questions related to geographic distribution, epidemiology and control, focusing on strategies that use genetic resistance.

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Characterization and genetic distance analysis of isolates of Peronosclerospora sorghi using AFLP fingerprinting

Cited by 19 publications

References 25 publications

Molecular identification of isolates of Peronosclerospora sorghi from maize using PCR-based SCAR marker

Molecular identification of isolates of Peronosclerospora sorghi from maize using PCR-based SCAR marker

Sustainable Agriculture Reviews

Peronosclerospora sorghi, o agente etiológico do míldio do sorgo

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