Characterization and genetic variability of<i>Fusarium verticillioides</i>strains isolated from corn and sorghum in Brazil based on fumonisins production, microsatellites, mating type locus, and mating crosses

Silva, Valéria Nascimento da; Fernandes, Flora Maria de Campos; Cortéz, Adriana; Ribeiro, Deise Helena Baggio; Almeida, Adriana Palma de; Hassegawa, Regina H.; Corrêa, Benedito

doi:10.1139/w06-029

Cited by 22 publications

(20 citation statements)

References 19 publications

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“…Contrary results were obtained after the amplification of DNA from mating populations using MAT-allele specific primers (Silva et al 2006;Hawa et al 2013).…”

Section: Discussionmentioning

confidence: 91%

Isolation and characterization of Fusarium verticillioides from maize in eastern China

Qiu

Dong

et al. 2015

Eur J Plant Pathol

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In 2012, we isolated mycotoxigenic fungi from maize kernels sourced in Jiangsu and Anhui provinces, China. Fusarium spp. accounted for most isolates detected, followed by Aspergillus and Penicillium spp. Of the Fusarium species, Fusarium verticillioides was the most prevalent, as revealed by direct PCR of DNA from kernel samples, followed by F. proliferatum and F. graminearum. Most F. verticillioides strains contained the FUM1 gene, the product of which plays a key role in fumonisin biosynthesis. In terms of mating type idiomorphs, about 80 % of all strains carried the MAT-1 allele, and 20 % carried MAT-2. The genetic structures of 192 representative F. verticillioides isolates were determined via an analysis of eight simple sequence repeat markers. This analysis revealed highlevel gene diversity but low linkage disequilibrium in three populations grouped by the geographical areas in which they were collected. The three populations exhibited low-level genetic differentiation and high gene flow, indicating that geographical differences minimally influenced population differentiation; all three populations were potentially components of a larger, randomly mating population.

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“…Contrary results were obtained after the amplification of DNA from mating populations using MAT-allele specific primers (Silva et al 2006;Hawa et al 2013).…”

Section: Discussionmentioning

confidence: 91%

Isolation and characterization of Fusarium verticillioides from maize in eastern China

Qiu

Dong

et al. 2015

Eur J Plant Pathol

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“…Da Silva et al (2006) studied the genetic variability of 21 Fusarium verticilloides strains isolated from sorghum cultivated in the State of São Paulo, Brazil, using SPAR (Single Primer Amplification Reaction) markers while Divakara et al (2013) identified and characterized 27 Fusarium spp., isolated from 58 sorghum seed samples collected across India, sequencing the transcription elongation factor 1 alpha gene and analyzing the Inter Simple Sequence Repeat (ISSR) polymorphisms. Additionally, Priyanka et al (2014) studied the diverse distribution of 200 Aspergillus strains from a total of 320 samples of maize, paddy, sorghum and groundnut by species-specific PCR assays.…”

Section: Fungusmentioning

confidence: 99%

“…Therefore prevention of fungal growth effectively conduces to prevention of mycotoxin accumulation (Barros et al, 2008). A number of fungal species associated with sorghum, belonging mainly to the genera Fusarium (Fusarium verticillioides, Fusarium graminearum, Fusarium equiseti), Alternaria (Alternaria alternata), Aspergillus (Aspergillus flavus), and Penicillium (Penicillium funiculosum) have been reported to produce mycotoxins and contaminate sorghum (Alves dos Reis et al, 2010;Da Silva et al, 2006;Isakeit et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Toxigenic molds in Tunisian and Egyptian sorghum for human consumption

Lahouar

Crespo-Sempere

Marı́n

et al. 2015

Journal of Stored Products Research

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a b s t r a c tThe objective of this study was to characterize the mycoflora of sorghum grains commercialized in the Tunisian retail market and to identify aflatoxins (AFs), ochratoxin A (OTA) and zearalenone (ZEA) producing species. Sixty four samples of sorghum (37 samples of Tunisian sorghum and 27 samples of Egyptian sorghum) were analyzed. Dilution plating (CFU, colony forming units) was used for fungal enumeration. The isolation of mycobiota was carried out by plating of grains on PDA and malachite green medium. Aspergillus section Flavi and section Nigri and Fusarium isolates were sub-cultured in CYA to test their ability to produce AFs, OTA and ZEA, respectively. The selected Aspergillus section Flavi and section Nigri, Penicillium and Fusarium isolates were subjected to specific PCR assays using published speciesspecific primers. The results revealed the dominance of Fusarium (95.3%), followed by Aspergillus (87.2%) and Alternaria (81.2%) species. The fungal counts ranged from 100 to 1.3$10 4 CFU/g for Tunisian sorghum and from 100 to 5.7$10 3 CFU/g for Egyptian sorghum. Among Aspergillus section Flavi isolates identified by molecular biology, Aspergillus flavus was the most dominant (90.1%) while Aspergillus parasiticus represent 9.9% only. About Aspergillus section Nigri, results showed the dominance of Aspergillus niger aggregate species, including Aspergillus niger, Aspergillus tubingensis and other species. Among Fusarium species, Fusarium incarnatum was the most dominant in both Tunisian and Egyptian sorghum. Penicillium citrinum was the dominant Penicillium species in the studied samples. More than 890 isolates belonging to the genus Aspergillus and Fusarium were tested in order to test their capacity to produce AFs, OTA and ZEA. The percentage of mycotoxin producing isolates in Aspergillus section Flavi, A. section Nigri, and Fusarium was 30.0%, 4.6% and 11.1%, respectively.

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“…Moreover, some mycotoxins such as fumonisins produced by this pathogen have negative effects on consumers since they cause leukoencephalomalacia in horses (Leslie & Summerell 2006;Silva et al 2006).…”

Section: Fusarium Importancementioning

confidence: 99%

Fusarium-plant interaction: state of the art - a review

Dinolfo¹,

Castañares²,

Stenglein³

2017

Plant Prot. Sci.

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Dinolfo M.I., Castañares E., Stenglein S.A. (2017): Fusarium-plant interaction: state of the art -a review. Plant Protect. Sci., 53: 61-70.One of the most important genera able to develop diseases in cereals is Fusarium which not only produces losses by the fungal presence but also mycotoxin production harmful to human and animal consumers. In the environment, plants are continuously threatened by abiotic and biotic stresses. Among the latter, pathogens gained importance mainly due to their ability to affect the plant fitness. To protect against potential attacks, plants have developed strategies in which phytohormones have an essential role. In plant-pathogen interactions, salicylic acid, ethylene, and jasmonates are the most important, but there are also auxins, gibberellins, abscisic acid, cytokinins, brassinosteroids, and peptide hormones involved in plant defence. The interaction between Fusarium species and plants used as models has been developed to allow understanding the plant behaviour against this kind of pathogen with the aim to develop several strategies to decrease the Fusarium disease effects.

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Characterization and genetic variability ofFusarium verticillioidesstrains isolated from corn and sorghum in Brazil based on fumonisins production, microsatellites, mating type locus, and mating crosses

Cited by 22 publications

References 19 publications

Isolation and characterization of Fusarium verticillioides from maize in eastern China

Isolation and characterization of Fusarium verticillioides from maize in eastern China

Toxigenic molds in Tunisian and Egyptian sorghum for human consumption

Fusarium-plant interaction: state of the art - a review

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