Characterization and genome-wide sequence analysis of an ectomycorrhizal fungus Pisolithus albus, a potential source for reclamation of degraded lands

Chot, Eetika; Suravajhala, Prashanth; Medicherla, Krishna Mohan; Reddy, S. M.

doi:10.1007/s13205-023-03483-5

Cited by 3 publications

(1 citation statement)

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“…Within mutualistic ECM fungi, the majority of our knowledge concerning potential roles for terpenes in communicating with plant hosts comes from transcriptomic and comparative genomic studies (Chot et al 2023 ; Lebreton et al 2022 ; Nosenko et al 2023 ; Ruytinx et al 2021 ). For example, terpene synthesis in the ECM fungus Piloderma croceum was found to be significantly regulated (Liao et al 2014 ).…”

Section: Introductionmentioning

confidence: 99%

Sesquiterpenes of the ectomycorrhizal fungus Pisolithus microcarpus alter root growth and promote host colonization

Plett,

Wojtalewicz,

Plett

et al. 2024

Mycorrhiza

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Trees form symbioses with ectomycorrhizal (ECM) fungi, maintained in part through mutual benefit to both organisms. Our understanding of the signaling events leading to the successful interaction between the two partners requires further study. This is especially true for understanding the role of volatile signals produced by ECM fungi. Terpenoids are a predominant class of volatiles produced by ECM fungi. While several ECM genomes are enriched in the enzymes responsible for the production of these volatiles (i.e., terpene synthases (TPSs)) when compared to other fungi, we have limited understanding of the biochemical products associated with each enzyme and the physiological impact of specific terpenes on plant growth. Using a combination of phylogenetic analyses, RNA sequencing, and functional characterization of five TPSs from two distantly related ECM fungi (Laccaria bicolor and Pisolithus microcarpus), we investigated the role of these secondary metabolites during the establishment of symbiosis. We found that despite phylogenetic divergence, these TPSs produced very similar terpene profiles. We focused on the role of P. microcarpus terpenes and found that the fungus expressed a diverse array of mono-, di-, and sesquiterpenes prior to contact with the host. However, these metabolites were repressed following physical contact with the host Eucalyptus grandis. Exposure of E. grandis to heterologously produced terpenes (enriched primarily in $$\gamma$$ γ -cadinene) led to a reduction in the root growth rate and an increase in P. microcarpus–colonized root tips. These results support a very early putative role of fungal-produced terpenes in the establishment of symbiosis between mycorrhizal fungi and their hosts.

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