Characterization and growth conditions of <scp>Vibrio parahaemolyticus</scp> strains with different virulence degrees that cause acute hepatopancreatic necrosis disease in <scp>Litopenaeus vannamei</scp>

Soto-Rodríguez, Sonia A.; Lozano‐Olvera, Rodolfo; Palacios‐Gonzalez, Daniel A.; Bolan‐Mejia, Carmen; Rendon‐Aguilar, Karla G.

doi:10.1111/jwas.12617

Cited by 19 publications

(8 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Soto‐Rodriguez et al . (2019) compared the growth kinetics of three Vp AHPND strains with distinct virulence and one nonpathogenic strain at different pH and NaCl concentration. However, no relation between the bacterial growth and degree of virulence was observed.…”

Section: Introductionmentioning

confidence: 99%

Coordination of primary metabolism and virulence factors expression mediates the virulence of Vibrio parahaemolyticus towards cultured shrimp ( Penaeus vannamei )

Yang

Zou

et al. 2020

J. Appl. Microbiol.

View full text Add to dashboard Cite

Aims Acute hepatopancreatic necrosis disease (AHPND) caused by Vibrio parahaemolyticus has emerged as a severe bacterial disease of cultured shrimp. To identify the key virulence factors, two AHPND‐causing V. parahaemolyticus (VpAHPND) strains (123 and 137) and two non‐VpAHPND strains (HZ56 and ATCC 17082) were selected. Methods and Results Challenge tests showed that the four strains exhibited different virulence towards shrimp with cumulative mortalities at 48 h postinfection (hpi) ranging from 10 to 92%. The expression of pirABVP in strain 123 and 137 was not significantly different. Genomic analysis revealed that the two VpAHPND strains contain a plasmid with the PirABVP toxins (pirABVP) flanked by the insertion sequence (ISVal1) that has been identified in various locations of chromosomes in VpAHPND strains. The two VpAHPND strains possessed almost identical virulence factors, while ISVal1 disrupted three genes related to flagellar motility in strain 137. Phenotype assay showed that strain 123 possessed the highest growth rate and swimming motility, followed by strain 137, suggesting that the disruption of essential genes mediated by ISVal1 significantly affected the virulence level. Transcriptome analysis of two VpAHPND strains (123 and 137) further suggested that virulence genes related to the capsule, flagella and primary metabolism were highly expressed in strain 123. Conclusions Here for the first time, it is demonstrated that the virulence of VpAHPND is not only determined by the expression of pirABVP, but also is mediated by ISVal1 which affects the genes involved in flagellar motility and primary metabolism. Significance and Impact of the Study The genomic and transcriptomic analysis of VpAHPND strains provides valuable information on the virulence factors affecting the pathogenicity of VpAHPND.

show abstract

Section: Introductionmentioning

confidence: 99%

Coordination of primary metabolism and virulence factors expression mediates the virulence of Vibrio parahaemolyticus towards cultured shrimp ( Penaeus vannamei )

Yang

Zou

et al. 2020

J. Appl. Microbiol.

View full text Add to dashboard Cite

show abstract

“…In this study, mortality was higher at lower saline concentration, finding a positive correlation with the expression of pirA Vp gene. Although more experiments are needed to determine the influence of salinity on the expression of pirAB Vp , these experiments corroborate that the toxin can be expressed at different salinities [82] and that the management of saline concentration in shrimp culture can be an important factor to control Vp infectivity.…”

Section: Environmental Factorsmentioning

confidence: 58%

PirAB from Vibrio parahaemolyticus Exhibits a Binary Toxin Action with Bactericidal Activity and Cytotoxicity in Penaeus vannamei

Soto-Rodríguez¹,

Lozano‐Olvera²,

Montfort³

et al. 2022

Preprint

Self Cite

View full text Add to dashboard Cite

PirAB is a binary protein complex secreted by specific strains of Vibrio parahaemolyticus (Vp) that harbor the pVA1 virulence plasmid and express PirAVp and PirBVp toxins. PirABVp causes acute hepatopancreatic necrosis disease (AHPND), a newly emergent penaeid shrimp disease that can cause 70–100% mortality and has resulted in great economic losses since its appearance. The cy-totoxic effect of PirABVp on the epithelial cell of the shrimp hepatopancreas has been extensively reported. Our studies found that the PirBVp subunit has lectin activity and recognizes mucin-like O-glycosidic structures in the shrimp hepatopancreas. The PirAVp subunit may have a stabiliza-tion function of the binary complex. However, we also found that Vp AHPND changes the water microbiota community structure and causes a significant reduction in several bacteria, especially Neptuniibacter spp. We propose that the PirABvp toxin could exhibit a dual role: damage the shrimp hepatopancreas and kill surrounding bacteria.

show abstract

“…The difference in the final survival lay in the fact that V. parahaemolyticus is a halophilic bacterium which reproduces more efficiently in environments with high salinities. Soto‐Rodriguez et al., (2019) found that Vp AHPND bacteria are able to reproduce in salinities between 5 ppt and 100 ppt, while Lekshmy et al., (2014) determined that there is a positive correlation between salinity and bacterial density in Vibrio parahaemolyticus isolates. It is possible that the increased bacterial density led to the production of a greater amount of pirAB toxin at the higher salinities.…”

Section: Discussionmentioning

confidence: 99%

Pathogenicity of Acute Hepatopancreatic Necrosis Disease (AHPND) on the freshwater prawn,Macrobrachium rosenbergii, and Pacific White Shrimp,Penaeus vannamei, at various salinities

Schofield

Noble

Aranguren

et al. 2020

Aquaculture Research

View full text Add to dashboard Cite

Acute hepatopancreatic necrosis disease (AHPND), caused by Vibrio parahaemolyticus (Vp AHPND), is a lethal disease to Penaeus vannamei shrimp. To determine the pathogenicity of Vp AHPND to giant river prawn (Macrobrachium rosenbergii), three immersion challenges were performed at a range of salinities. In the first trial, M. rosenbergii were challenged to observe the extent of AHPND infection. The second trial had P. vannamei exposed to Vp AHPND at low salinities to observe the effect. Results showed the Vp AHPND caused infection in P. vannamei at 5–20 ppt with survival rates ranging from 0% to 65%. The third trial simultaneously exposed M. rosenbergii and P. vannamei to Vp AHPND to determine the effect of AHPND in two species while in identical environments. In trials I and III, M. rosenbergii did not show any mortality and no pathology indicative of AHPND were noted. In challenges II and III, P. vannamei exhibited 0% to 65% survival and were AHPND positive by histology and PCR. These findings indicate that M. rosenbergii held in salinities of 20 ppt and lower are not susceptible to AHPND at a dose of 1x106 CFU/ml, which was lethal to P. vannamei at all salinities tested.

show abstract

Characterization and growth conditions of Vibrio parahaemolyticus strains with different virulence degrees that cause acute hepatopancreatic necrosis disease in Litopenaeus vannamei

Cited by 19 publications

References 43 publications

Coordination of primary metabolism and virulence factors expression mediates the virulence of Vibrio parahaemolyticus towards cultured shrimp ( Penaeus vannamei )

Coordination of primary metabolism and virulence factors expression mediates the virulence of Vibrio parahaemolyticus towards cultured shrimp ( Penaeus vannamei )

PirAB from <em>Vibrio parahaemolyticus</em> Exhibits a Binary Toxin Action with Bactericidal Activity and Cytotoxicity in <em>Penaeus vannamei</em>

Pathogenicity of Acute Hepatopancreatic Necrosis Disease (AHPND) on the freshwater prawn,Macrobrachium rosenbergii, and Pacific White Shrimp,Penaeus vannamei, at various salinities

Contact Info

Product

Resources

About